6533b861fe1ef96bd12c566a

RESEARCH PRODUCT

Confocal scanning microscope using a CCD camera as a pinhole-detector system

Ana DoblasEmilio Sánchez-ortigaManuel Martínez-corralA. CalatayudGenaro Saavedra

subject

MicroscopeMaterials sciencePixelOptical sectioningbusiness.industryConfocalDetectorComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISIONlaw.inventionOpticslawConfocal microscopyMicroscopyPinhole (optics)business

description

We report a scheme for a detector system of confocal microscopes. In our scheme the pinhole and the large area detector are subtituted by a CCD camera. The numerical integration of the intensities acquired by the active pixels emulates the signal acquired by the detector. To demonstrate the utility of the system we efficiently performed an experiment of substractive-image confocal microscopy

https://doi.org/10.1109/wio.2011.5981473