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RESEARCH PRODUCT

Novel DNA Methylation Sites Influence GPR15 Expression in Relation to Smoking

Philipp S. WildJulia KrauseCaroline RöthemeierSonja KunzeThomas MünzelMatthias MichalJustus StenzigChristian P. MüllerBlankenberg StefanTanja ZellerTina HaaseMahir KarakasMelanie WaldenbergerFederico MariniKarl J. LacknerNorbert Pfeiffer

subject

0301 basic medicineMalemedicine.medical_specialtyGpr15 ; Smoking ; Biomarker ; Dna MethylationReceptors Peptidemedicine.medical_treatmentPopulationlcsh:QR1-502BiologyBiochemistrylcsh:MicrobiologyArticleReceptors G-Protein-Coupled03 medical and health sciences0302 clinical medicineInternal medicineGene expressionmedicineHumansRNA MessengerReceptoreducationMolecular BiologyAgedRegulation of gene expressioneducation.field_of_studyDNA methylationSmokingMethylationMiddle Aged030104 developmental biologyEndocrinologyCpG siteGene Expression RegulationGenetic LociDNA methylationSmoking cessationGPR15biomarkerFemale030217 neurology & neurosurgery

description

Smoking is a major risk factor for cardiovascular diseases and has been implicated in the regulation of the G protein-coupled receptor 15 (GPR15) by affecting CpG methylation. The G protein-coupled receptor 15 is involved in angiogenesis and inflammation. An effect on GPR15 gene regulation has been shown for the CpG site CpG3.98251294. We aimed to analyze the effect of smoking on GPR15 expression and methylation sites spanning the GPR15 locus. DNA methylation of nine GPR15 CpG sites was measured in leukocytes from 1291 population-based individuals using the EpiTYPER. Monocytic GPR15 expression was measured by qPCR at baseline and five-years follow up. GPR15 gene expression was upregulated in smokers (beta (&szlig

yearjournalcountryeditionlanguage
2018-08-01Biomolecules
10.3390/biom8030074http://europepmc.org/articles/PMC6163736