6533b86cfe1ef96bd12c8070

RESEARCH PRODUCT

Determination of albendazole and ivermectin residues in cattle and poultry-derived samples from India by micellar liquid chromatography

subject

description

We have developed a method, based on micellar liquid chromatography, to determine albendazole and ivermectin in dairy products and biological waste from bovine, as well as edible tissues from poultry. Anthelmitics were resolved in less than 10 min using a C18 column and a mobile phase of 0.15 mol/L sodium dodecyl sulfate – 6% 1-pentanol at buffered at pH 7 with a 0.01 M phosphate salt, running under isocratic mode at 1 mL/min. Detection was by absorbance at 292 nm. Method was successfully validated following official validation guidelines, in terms of: selectivity, sensitivity, calibration range (0.0125−0.5 mg/kg to 25−50 mg/kg), linearity (r2 > 9990), trueness (86.3–105.6%), precision (<12.2 %), carry-over effect, robustness and stability. Procedure was easy-to-conduct, safe, ecofriendly, economical and with a high sample throughput. It can be used as screening method prior to a confirmatory one, like liquid chromatography-mass spectrometry. Finally, it was applied to incurred samples. In nearly half of the food samples, the analytes were found at concentrations (albendazole 0.20–4.1 mg/kg and ivermectin, 0.31–8.73 mg/kg) over the maximum residue limits stated by international regulations. This exposes consumer to the risk of suffering from severe diseases and points out an inappropriate administration of anthelmitic-based treatments.