6533b86cfe1ef96bd12c8208

RESEARCH PRODUCT

Transient Multivalent Nanobody Targeting to CD206-Expressing Cells via PH-Degradable Nanogels

Bruno G. De GeestHansjörg SchildJudith StickdornAna Rita Pombo AntunesStephan GrabbeSana M. ArnoukLutz NuhnEvangelia BolliJo A. Van GinderachterAlexandra Van DriesscheMaximilian Scherger

subject

0301 basic medicineEndosomeNanogels02 engineering and technologyConjugated systemArticleM2 macrophage03 medical and health sciencesHumansReversible addition−fragmentation chain-transfer polymerizationlcsh:QH301-705.5targetingchemistry.chemical_classificationRAFT polymerizationChinese hamster ovary cellGeneral MedicinePolymerHydrogen-Ion Concentrationmultivalency021001 nanoscience & nanotechnologynanobody030104 developmental biologyTAMchemistryCD206lcsh:Biology (General)nanogelclick chemistryClick chemistryBiophysicsNanocarriers0210 nano-technologyNanogel

description

To target nanomedicines to specific cells, especially of the immune system, nanobodies can be considered as an attractive tool, as they lack the Fc part as compared to traditional antibodies and, thus, prevent unfavorable Fc-receptor mediated mistargeting. For that purpose, we have site-specifically conjugated CD206/MMR-targeting nanobodies to three types of dye-labeled nanogel derivatives: non-degradable nanogels, acid-degradable nanogels (with ketal crosslinks), and single polymer chains (also obtained after nanogel degradation). All of them can be obtained from the same reactive ester precursor block copolymer. After incubation with na&iuml

yearjournalcountryeditionlanguage
2020-10-01Cells
10.3390/cells9102222https://www.mdpi.com/2073-4409/9/10/2222