6533b86cfe1ef96bd12c8bee
RESEARCH PRODUCT
Detection of lactobacillic acid in low erucic rapeseed oil--A note of caution when quantifying cyclic fatty acid monomers in vegetable oils.
William W. ChristieOlivier BerdeauxPierre LambeletCindie FournierJean-louis SébédioStéphane Grégoiresubject
RapeseedFraction (chemistry)Mass spectrometry01 natural sciencesBiochemistryHigh-performance liquid chromatographyGas Chromatography-Mass SpectrometryTHERMAL DEGRADATIONFatty Acids Monounsaturated03 medical and health scienceschemistry.chemical_compoundLOW ERUCIC ACID RAPESEED OIL[SDV.IDA]Life Sciences [q-bio]/Food engineering1112-METHYLENE-OCTADECANOIC ACIDOrganic chemistryPlant Oils[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringMolecular Biology030304 developmental biologychemistry.chemical_classification0303 health sciencesChromatographyChemistryCYCLIC FATTY ACID MONOMERS010401 analytical chemistryOrganic ChemistryFatty AcidsFatty acidMASS-SPECTROMETRYCell BiologySunflower0104 chemical sciencesErucic acidRapeseed OilQuantitative analysis (chemistry)description
International audience; The purpose of this work was to identify an unknown component which has been detected during the analysis of cyclic fatty acid monomers (CFAMs) in low erucic acid rapeseed oils (LEAR). A sample of crude LEAR was transformed into fatty acid methyl esters (FAMEs) and hydrogenated using PtO2. The hydrogenated sample was fractionated by reversed-phase high-performance liquid chromatography (RP-HPLC) and the fraction containing the CFAMs transformed into picolinyl esters. Analysing these picolinyl derivatives by gas-liquid chromatography coupled to mass spectrometry (GC-MS) showed that the unknown product observed in LEAR is the 11,12-methylene-octadecanoic acid. This cyclic fatty acid was also found in crude LEAR and in the corresponding seeds but was not detected in crude soya and sunflower oils. As this acid is present in the same fraction as CFAMs, known to be formed during heat treatment, great care must therefore be taken for not including it when quantifying CFAMs. It is thus necessary to verify by mass spectrometry the structures of the CFAMs in the isolated cyclic fatty acid fraction prior to quantification.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-01-01 | Chemistry and physics of lipids |