6533b86cfe1ef96bd12c8ca4

RESEARCH PRODUCT

Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method

Angelina AlongiAndrea MacalusoRosa PitonzoNicola CiceroVita Di StefanoVincenzo FerrantelliGabriella CancemiVita Giaccone

subject

UHPLC –ESI–MS/MSβ2-agonist0301 basic medicineElectrospray030109 nutrition & dieteticsChromatographycattle hair; Commission Decision 2002/657/EC; UHPLC–ESI–MS/MS; β2-agonists; Plant Science; Biochemistry; Analytical Chemistry; Organic ChemistryChemistryβ2 agonistsElectrospray ionization010401 analytical chemistryOrganic ChemistryPlant ScienceContaminationTandem mass spectrometry01 natural sciencesBiochemistry0104 chemical sciencesAnalytical Chemistry03 medical and health sciencesCommission Decision 2002/657/ECcattle hairAdrenergic beta-AgonistsUltra high performanceDecision limit

description

A simple and efficient method was developed for simultaneous analysis of five illegal residual β2-agonists in cattle hair. β2-Agonists were quantified by ultra high performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry operating in positive multiple-reaction monitoring mode. The method was validated as quantitative confirmatory method according to the EU Decision 2002/657/EC: instrumental linearity, specificity, precision, recovery, decision limit (CCα) and detection capability (CCβ) were evaluated. The recovery were greater than 90% and the method appeared suitable for the control of these β2-agonists in cattle hair samples with LOQ values between 4.9 and 5.5 μg/kg. This method could represent a simple and cheap approach to confirm β2-agonists contamination of cattle for feeding in a not invasive way and before slaughter operations.

yearjournalcountryeditionlanguage
2016-01-01Natural Product Research
https://doi.org/10.1080/14786419.2016.1190718