6533b86dfe1ef96bd12ca947
RESEARCH PRODUCT
Transmembrane electrochemistry of erythrocytes: Direct electrochemical test for detecting hemolysis in whole blood
Clara Doménech-casasúsAntonio Doménech-carbóDavid RamosEva VillamónGerardo Cebrián-torrejónIrene Lunasubject
Analytical chemistry02 engineering and technologyGlassy carbon010402 general chemistryElectrochemistry01 natural sciencesScanning electrochemical microscopyBlood plasmaMaterials ChemistrymedicineElectrochemistryElectrical and Electronic EngineeringInstrumentationWhole bloodHemolysis indexDetection limitChromatographyChemistryMetals and AlloysVenous blood021001 nanoscience & nanotechnologyCondensed Matter Physicsmedicine.diseaseHemolysis0104 chemical sciencesSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsVenous bloodFinger blood0210 nano-technologydescription
A rapid method for detecting hemolysis in whole blood based on a direct electrochemical assay either in venous blood and finger blood, respectively at glassy carbon and screen-printed graphite electrodes is described. The presence of hemolysis is detected from characteristic voltammetric signatures associated to Fe-heme units in healthy and hemolyzed erythrocytes. The voltammetric response of blood was also investigated using scanning electrochemical microscopy (SECM) and scanning tunneling microscopy (STM) and involved transmembrane electrochemistry of erythrocytes superimposed to molecular electrochemistry of heme-containing proteins and heme fragments in the plasma. Voltammetric testing of hemolysis was in agreement with conventional screening in blood plasma in 100% of studied cases leading to a detection limit for the hemolysis index (HI) of 0.02 g L-1 of free hemoglobin. (C) 2015 Elsevier B.V. All rights reserved.
year | journal | country | edition | language |
---|---|---|---|---|
2016-04-01 |