6533b86efe1ef96bd12cb671
RESEARCH PRODUCT
Generalization of seizures parallels the formation of "dark" neurons in the hippocampus and pontine reticular formation after focal-cortical application of 4-aminopyridine (4-AP) in the rat.
Zsuzsanna SzepesiGergely OrbanPéter BaracskayAndrás CzurkóGábor Juhászsubject
Male* Dark neuronMicroinjections* Epilepsy; * Dark neuron; * Hippocampus; * Pontine reticular formation; * Cell injury; * Animal model; * Neurogliaform cell; * Astrocyte; * Status epilepticusHippocampus* Status epilepticusStatus epilepticusReticular formationHippocampusSettore BIO/09 - FisiologiaRats Sprague-DawleyEpilepsySeizuresPonsConvulsionmedicinePotassium Channel BlockersAnimals4-AminopyridineMolecular Biology* Animal modelNeurons* Pontine reticular formationBehavior AnimalChemistryGeneral NeuroscienceReticular Formation* Neurogliaform cellElectroencephalographyParamedian pontine reticular formation* Hippocampumedicine.disease* Cell injuryRats* Astrocyte* Epilepsymedicine.anatomical_structureMossy Fibers HippocampalNeurology (clinical)Epileptic seizureNeuronmedicine.symptomNeuroscienceDevelopmental Biologydescription
Abstract Distribution and time course of the occurrence of “dark” neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K + channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats for 40 min. Thereafter, the anesthesia was discontinued and the behavioral signs of the epileptic seizure activity were observed. The presence of “dark” neurons was demonstrated by the sensitive silver method of Gallyas in rats sacrificed at 0, 3 and 6 h after the end of the 4-AP crystal application. The EEG activity was recorded in the rats with longer survival times. The EEG analysis revealed the generalization of the epileptic seizures. We found that the formation of “dark” neurons in the hippocampus and the pontine reticular formation paralleled the generalization of the seizures.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-02-07 |