6533b86efe1ef96bd12cbf0e
RESEARCH PRODUCT
Copper carbonate as a solid-bed reactor for spectrophotometric determination of doxycycline and oxytetracycline in an unsegmented continuous flow assembly
J. Martínez CalatayudJ.l. Lopez Pazsubject
PolymersCalibration curveClinical BiochemistryCarbonatesPharmaceutical Sciencechemistry.chemical_elementOxytetracyclineOxytetracyclineDoxycycline HyclateAnalytical ChemistrySpectrophotometryDrug DiscoverymedicineSpectroscopyAntibacterial agentchemistry.chemical_classificationFlow injection analysisChromatographymedicine.diagnostic_testPolymerHydrogen-Ion ConcentrationCopperchemistryDoxycyclineCalibrationFlow Injection AnalysisSpectrophotometry UltravioletCoppermedicine.drugdescription
The FIA-spectrophotometric determination of doxycycline was carried out by reaction of the drug with cupric ions entrapped in a polymeric material in a packed-bed reactor: the complex formed was then injected into a manifold with an alkaline solution as carrier. The developed colour was monitored at 395.0 nm. The method was applied to the determination of doxycycline in different pharmaceutical formulations. The calibration graph for doxycycline hyclate was linear over the range 10.0-80.0 mg ml-1 (n = 8) with a relative standard deviation of 1.4% (at 25 mg ml-1) and a sample throughput of 128 h-1. The proposed procedure was also applied to the determination of oxytetracycline in pharmaceutical formulations.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-11-01 | Journal of Pharmaceutical and Biomedical Analysis |