6533b86efe1ef96bd12cc949

RESEARCH PRODUCT

Dissociation-constants of metat-ion-complexes with alkaline phosphatase from pig kidney.

B. P. AckermannJ. Ahlers

subject

inorganic chemicalsPharmacologyManganeseChemistryCations DivalentSwinePig kidneyIronInorganic chemistryCell BiologyAlkaline PhosphataseKidneyIonDissociation constantCellular and Molecular NeuroscienceKineticsZincMolecular MedicineAlkaline phosphataseAnimalsCalciumMagnesiumMolecular Biology

description

Using metal-ion buffers it was possible to remove Zn2+, Mg2+ and Mn2+ ions of pig kidney alkaline phosphatase reversibly. The dissociation constants obtained are KEMg: 4 X 10(-7) M, KEMn: 4 X 10(-8) M and KEZn: 8 X 10(-13) M (22 degrees C, pH: 9.6, mu: 0.07).

yearjournalcountryeditionlanguage
1976-03-01Experientia
10.1007/bf01940795https://pubmed.ncbi.nlm.nih.gov/1253893