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RESEARCH PRODUCT
4-dimethylamino-3′,4′-dimethoxychalcone downregulates iNOS expression and exerts anti-inflammatory effects
María José AlcarazMaría Luisa FerrándizAmalia UbedaFelipe HerenciaGricela LoboJosé N. DomínguezJaime CharrisIsabel Guillénsubject
Anti-Inflammatory AgentsNitric Oxide Synthase Type IIPharmacologyCarrageenanNitric OxideBiochemistryGene Expression Regulation EnzymologicNitric oxideMicechemistry.chemical_compoundChalconeChalconesSuperoxidesIn vivoPhysiology (medical)AnimalsEdemaEnzyme InhibitorsRespiratory BurstInflammationTironbiologySuperoxideZymosanZymosanFree Radical ScavengersNitric oxide synthaseOxidative StresschemistryBiochemistryEicosanoidLuminescent Measurements12-Dihydroxybenzene-35-Disulfonic Acid Disodium SaltMacrophages Peritonealbiology.proteinFemaleTumor necrosis factor alphaNitric Oxide Synthasedescription
Abstract Reactive oxygen and nitrogen species contribute to the pathophysiology of inflammatory conditions. We have studied the effects of a novel superoxide scavenger, 4-dimethylamino-3′,4′-dimethoxychalcone (CH11) in macrophages and in vivo. CH11 has been shown to inhibit the chemiluminescence induced by zymosan in mouse peritoneal macrophages and the cytotoxic effects of superoxide. In the same cells, the modulation by superoxide of nitric oxide (NO) production in response to zymosan was investigated. CH11 was more effective than the membrane-permeable scavenger Tiron for inhibition of inducible nitric oxide synthase (iNOS) protein expression and nitrite production. We have shown that CH11 inhibited chemiluminescence in vivo, as well as cell migration, and eicosanoid and tumor necrosis factor-α (TNF-α) levels in the mouse air pouch injected with zymosan. This chalcone derivative also exerted anti-inflammatory effects in the carrageenan paw oedema.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-01-03 | Free Radical Biology and Medicine |