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RESEARCH PRODUCT

Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism

Janis LiepinsAgnese KokinaArmands VigantsMark G. PoolmanPeteris ZikmanisRita ScherbakaJekaterina MartynovaI MozgaEgils StalidzansHassan B. HartmanDavid A. FellAgris Pentjuss

subject

GlycerolModels Molecular0301 basic medicinePhenylalanineSuccinic AcidEthyl acetateGlutamic AcidLactoseBioengineeringAcetatesXyloseApplied Microbiology and BiotechnologyMetabolic engineeringIndustrial MicrobiologyKluyveromyces03 medical and health scienceschemistry.chemical_compoundOxygen ConsumptionKluyveromyces marxianusGlycerolBiomassFood scienceXyloseEthanolbiologyInulinReproducibility of ResultsSubstrate (chemistry)Phenylethyl Alcoholbiology.organism_classificationYeastCulture MediaGlucose030104 developmental biologyMetabolic EngineeringchemistryBiochemistryYield (chemistry)CalibrationLactatesBiotechnology

description

Abstract The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism’s potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.

yearjournalcountryeditionlanguage
2016-10-20Journal of Industrial Microbiology and Biotechnology
https://doi.org/10.1007/s10295-017-1946-8