6533b86ffe1ef96bd12cddfe
RESEARCH PRODUCT
Heterogeneous lack of expression of the tumour suppressor PTEN protein in human neoplastic tissues.
Josema TorresJavier García-condeRafael PulidoAntonio Llombart-boschSamuel NavarroAna LluchFrancisca RipollIsabel RogláJavier Cerverasubject
Cancer ResearchTumor suppressor genemedicine.drug_classMonoclonal antibodyEpitopelaw.inventionMicelawNeoplasmsGene expressionmedicineTumor Cells CulturedPTENAnimalsHumansMice Inbred BALB CbiologyTumor Suppressor ProteinsPTEN PhosphohydrolaseCancerAntibodies Monoclonalmedicine.diseaseMolecular biologyImmunohistochemistryPhosphoric Monoester HydrolasesNeoplasm ProteinsOncologybiology.proteinCancer researchSuppressorImmunohistochemistrydescription
PTEN, a tumour suppressor gene located at chromosome 10q23 and commonly mutated or deleted in a variety of tumours, encodes a dual-specific/phosphatidylinositol-3,4,5-triphosphate (PIP3) phosphatase. We report the generation of an anti-PTEN monoclonal antibody (MAb) that recognises an epitope at the C-terminus of PTEN, and describe the heterogeneous lack of expression of the PTEN protein in human tumour tissues, as demonstrated by immunohistochemical methods. Our anti-PTEN MAb provides a useful tool for the study of PTEN protein expression in tumour samples, in the search for tumour prognostic molecular markers.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-02-13 | European journal of cancer (Oxford, England : 1990) |