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RESEARCH PRODUCT

Molecular Characterization of a Chromosomal Rearrangement Involved in the Adaptive Evolution of Yeast Strains

Amparo QuerolJosé E. Pérez-ortínEladio BarrioSergi Puig

subject

Genetic MarkersSaccharomyces cerevisiae ProteinsLetterChromosomal rearrangementsAnion Transport ProteinsGenes FungalMolecular Sequence DataSaccharomyces cerevisiaeSaccharomyces cerevisiaeChromosomal rearrangementSaccharomycesGenètica molecularTranslocation GeneticEvolution MolecularSaccharomycesGene FrequencySpecies SpecificityGeneticsVinificationDNA FungalGeneGenetics (clinical)Wine yeastsGene RearrangementRecombination GeneticGeneticsBase SequencebiologyGene rearrangementbiology.organism_classificationYeastYeast in winemakingChromosomes FungalGenome FungalPloidy

description

Wine yeast strains show a high level of chromosome length polymorphism. This polymorphism is mainly generated by illegitimate recombination mediated by Ty transposons or subtelomeric repeated sequences. We have found, however, that the SSU1-R allele, which confers sulfite resistance to yeast cells, is the product of a reciprocal translocation between chromosomes VIII and XVI due to unequal crossing-over mediated by microhomology between very short sequences on the 5' upstream regions of the SSU1 and ECM34 genes. We also show that this translocation is only present in wine yeast strains, suggesting that the use for millennia of sulfite as a preservative in wine production could have favored its selection. This is the first time that a gross chromosomal rearrangement is shown to be involved in the adaptive evolution of Saccharomyces cerevisiae.

yearjournalcountryeditionlanguage
2002-10-01Genome Research
https://doi.org/10.1101/gr.436602