6533b870fe1ef96bd12cf30f
RESEARCH PRODUCT
Regulation of vitellogenesis in Nereis virens (Annelida: Polychaeta): effect of estradiol-17beta on eleocytes.
U HoegerN RebscherJavier García-alonsosubject
Malemedicine.medical_specialtyfood.ingredientPhysiologyBiochemistryOogenesisVitellogeninVitellogeninsfoodYolkInternal medicinemedicineEndocrine systemAnimalsNereididaeMolecular BiologySex CharacteristicsbiologyEstradiolReproductionVitellogenesisPolychaetabiology.organism_classificationEndocrinologyGerm Cellsbiology.proteinCoelomFemaleVitellogenesisHormonedescription
Abstract In the marine polychaete Nereis virens , the yolk protein precursor vitellogenin (Vg) is synthesized in specialized coelomic cells (eleocytes) during oogenesis. This process was visualized by immunohistochemistry using antibodies raised against the yolk protein. Transversal sections from male and female worms confirmed that eleocytes from females but not from males produce Vg. In order to investigate the hormonal regulation of Vg synthesis, eleocytes were incubated in vitro with estradiol-17β (E 2 ) at a concentration of 1 μg/l for up to three days. A strong increase in Vg secretion was detected by ELISA in culture media of treated eleocytes from vitellogenic females. In contrast, no response to the hormonal treatment was detectable in immature worms. Our results showed that Vg synthesis is under a complex regulation, which involves endocrine factors like estrogens. The role of E 2 in vitellogenesis of N. virens rather resembles the situation found in vertebrate than the one in insects.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-05-27 | Comparative biochemistry and physiology. Part A, Molecularintegrative physiology |