6533b870fe1ef96bd12cf3c8
RESEARCH PRODUCT
Determination of barbiturates in urine by micellar liquid chromatography and direct injection of sample.
M.j. Medina-hernándezRosa María Villanueva-camañasYolanda Martín-bioscaSalvador Sagradosubject
AmobarbitalClinical BiochemistryPharmaceutical Science1-PropanolBarbitalAnalytical Chemistrychemistry.chemical_compoundColumn chromatographyDrug DiscoverymedicineHumansSample preparationPentobarbitalSpectroscopyDetection limitBarbituric acidChromatographyButabarbitalHydrogen-Ion ConcentrationchemistryAlkanesulfonic AcidsMicellar liquid chromatographyBarbitalPhenobarbitalBarbituratesCalibrationAmobarbitalmedicine.drugChromatography Liquiddescription
Abstract A liquid chromatographic procedure for the determination of six barbiturates (barbital, diallyl barbituric acid, phenobarbital, butabarbital, amobarbital and pentobarbital) in urine samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C 18 analytical column and a guard column of similar characteristics. The UV detector was set at 240 nm. A study to select adequate composition of the micellar mobile phase for the separation of these compounds in urine samples is performed. Maximum resolution was achieved with a 0.07 M sodium dodecylsulphate-0.3% propanol at pH 7.4 eluent. Limits of detection at 240 nm were ranged between 0.13 μg ml −1 for diallyl barbituric acid and 2.7 μg ml −1 for amobarbital. The procedure allows for the determination of these compounds in 20 minutes, it does not require prior a sample preparation step and it can be very useful to the investigation of intoxication.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-03-07 | Journal of pharmaceutical and biomedical analysis |