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RESEARCH PRODUCT
Acid trehalase is involved in intracellular trehalose mobilization during postdiauxic growth and severe saline stress in Saccharomyces cerevisiae.
Emilia MatallanaEmilia MatallanaJosé V. Gimeno-alcañizElena GarreElena GarreRoberto Pérez-torradosubject
SalinitySaccharomyces cerevisiae ProteinsTranscription GeneticSaccharomyces cerevisiaeMutantTrehalase activitySaccharomyces cerevisiaeBiologyApplied Microbiology and BiotechnologyMicrobiologychemistry.chemical_compoundOsmotic PressureGene Expression Regulation FungalTrehalaseTrehalaseProtein kinase AGene Expression ProfilingTrehaloseGeneral Medicinebiology.organism_classificationTrehaloseYeastBiochemistrychemistryMitogen-Activated Protein KinasesIntracellularGene Deletiondescription
The role of the acid trehalase encoded by the ATH1 gene in the yeast Saccharomyces cerevisiae is still unclear. In this work, we investigated the regulation of ATH1 transcription and found a clear involvement of the protein kinase Hog1p in the induction of this gene under severe stress conditions, such as high salt. We also detected changes in the acid trehalase activity and trehalose levels, indicating a role of the acid trehalase in intracellular trehalose mobilization. Finally, the growth analysis for different mutants in neutral and acid trehalases after high salt stress implicates acid trehalase activity in saline stress resistance.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-11-20 | FEMS yeast research |