6533b871fe1ef96bd12d1c50

RESEARCH PRODUCT

Simultaneous determination in situ of DNA fragmentation and 8-oxoguanine in human sperm.

Jaime GosálvezMarcos MeseguerNicolás GarridoMaría TamayoRebeca SantisoJosé Luis Fernández

subject

Maleendocrine systemGuanineDNA damageDNA FragmentationBiologychemistry.chemical_compoundPredictive Value of TestsHumansFragmentation (cell biology)reproductive and urinary physiologyurogenital systemHybridization probeObstetrics and GynecologyReproducibility of ResultsSpermMolecular biologySpermatozoa8-OxoguanineChromatinChromatinUp-RegulationOxidative StressReproductive MedicinechemistryMicroscopy FluorescenceDNA fragmentationReagent Kits DiagnosticDNABiomarkers

description

Deoxyribonucleic acid fragmentation and oxidative DNA damage were simultaneously determined in the same sperm cell, incubating with an 8-oxoguanine DNA probe on human spermatozoa processed by the sperm chromatin dispersion test. The assay was validated by incubation with agents that induce DNA fragmentation with or without oxidative base damage. In all samples examined, increased levels of 8-oxoguanine were present only in those spermatozoa with fragmented DNA, suggesting a link between both DNA damage types.

yearjournalcountryeditionlanguage
2009-04-29Fertility and sterility
10.1016/j.fertnstert.2009.07.969https://pubmed.ncbi.nlm.nih.gov/19748084