0000000000942870

AUTHOR

María Tamayo

showing 2 related works from this author

Swim-up procedure selects spermatozoa with longer telomere length.

2010

Telomere length and sperm DNA fragmentation were determined in sperm samples from 27 patients, using a quantitative PCR (Q-PCR) assay and the Sperm Chromatin Dispersion (SCD) test, respectively. Comparisons of the samples before and after swim-up processing demonstrated that this procedure selects a sperm population with longer average telomere size and lower frequency of sperm cells with fragmented DNA.

Maleendocrine systemeducation.field_of_studyurogenital systemHealth Toxicology and MutagenesisPopulationDNA FragmentationBiologyTelomereSpermMolecular biologySpermatozoaChromatinTelomerechemistry.chemical_compoundReal-time polymerase chain reactionchemistryGeneticsSperm MotilityDNA fragmentationHumanseducationMolecular Biologyreproductive and urinary physiologyDNAMutation research
researchProduct

Simultaneous determination in situ of DNA fragmentation and 8-oxoguanine in human sperm.

2009

Deoxyribonucleic acid fragmentation and oxidative DNA damage were simultaneously determined in the same sperm cell, incubating with an 8-oxoguanine DNA probe on human spermatozoa processed by the sperm chromatin dispersion test. The assay was validated by incubation with agents that induce DNA fragmentation with or without oxidative base damage. In all samples examined, increased levels of 8-oxoguanine were present only in those spermatozoa with fragmented DNA, suggesting a link between both DNA damage types.

Maleendocrine systemGuanineDNA damageDNA FragmentationBiologychemistry.chemical_compoundPredictive Value of TestsHumansFragmentation (cell biology)reproductive and urinary physiologyurogenital systemHybridization probeObstetrics and GynecologyReproducibility of ResultsSpermMolecular biologySpermatozoa8-OxoguanineChromatinChromatinUp-RegulationOxidative StressReproductive MedicinechemistryMicroscopy FluorescenceDNA fragmentationReagent Kits DiagnosticDNABiomarkersFertility and sterility
researchProduct