6533b871fe1ef96bd12d1c82

RESEARCH PRODUCT

Use of Flow Cytometry for Producing Tetraploids in Red Clover

Dace GraudaAija RebāneAldis JansonsIsaak RashalSarmīte Rancāne

subject

MultidisciplinaryGeneral interestmedicine.diagnostic_testSciencefungiQfood and beveragesFlow cytometryRed Clovermixoploidytrifolium pratensebreedingBotanymedicine

description

Abstract Sprouts of diploid red clover ‘Stendes Agrais’ were treated with 0.2% colchicine solution. The ploidy level of plants was detected by flow cytometry. Most of the treated plants were mixoploids and sterile. From progenies of fertile plants, mixoploids were selected that had a proportion of tetraploid cells higher than 60%. In the next generation, stable tetraploid plants were selected, and 49 lines were included in the breeding process.

10.2478/prolas-2020-0052https://doaj.org/article/ef688fae6c3a4e9fbe4f61fcf05b5fed