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RESEARCH PRODUCT
The nuclear protein Sge1 of Fusarium oxysporum is required for parasitic growth
Claude AlabouvetteMartijn RepErik M. M. MandersRingo Van WijkLinda ReijnenCaroline B. MichielseChantal OlivainSonja Boassubject
FusariumQH301-705.5[SDV]Life Sciences [q-bio]ImmunologyGenes FungalMolecular Sequence Datachampignon phytopathogèneMicrobiologyPlant RootsMicrobiologyHost-Parasite InteractionsFungal ProteinsFusariumSolanum lycopersicumVirologyGene Expression Regulation FungalFusarium oxysporumGeneticsAmino Acid SequenceBiology (General)Cloning MolecularMolecular BiologyVascular tissuePhylogenyWilt diseaseRegulation of gene expressionCell NucleusFungal proteinbiologyOrganisms Genetically ModifiedSequence Homology Amino AcidEffectorfungifood and beveragesNuclear ProteinsPathogenic fungusRC581-607Microbiology/Plant-Biotic Interactionsbiology.organism_classificationPathology/Molecular Pathology[SDE]Environmental SciencesParasitologyImmunologic diseases. AllergyResearch ArticleTranscription Factorsdescription
Dimorphism or morphogenic conversion is exploited by several pathogenic fungi and is required for tissue invasion and/or survival in the host. We have identified a homolog of a master regulator of this morphological switch in the plant pathogenic fungus Fusarium oxysporum f. sp. lycopersici. This non-dimorphic fungus causes vascular wilt disease in tomato by penetrating the plant roots and colonizing the vascular tissue. Gene knock-out and complementation studies established that the gene for this putative regulator, SGE1 (SIX Gene Expression 1), is essential for pathogenicity. In addition, microscopic analysis using fluorescent proteins revealed that Sge1 is localized in the nucleus, is not required for root colonization and penetration, but is required for parasitic growth. Furthermore, Sge1 is required for expression of genes encoding effectors that are secreted during infection. We propose that Sge1 is required in F. oxysporum and other non-dimorphic (plant) pathogenic fungi for parasitic growth.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-10-01 | PLoS Pathogens |