6533b872fe1ef96bd12d2d7c

RESEARCH PRODUCT

Molekülgrößenbestimmungen an makromolekularen Stoffen (Kritik und Vergleich der Methoden)

G. V. Schulz

subject

PharmacologyChemistryDiffusionExtrapolationThermodynamicsCell BiologyIdeal solutionLight scatteringCellular and Molecular NeuroscienceViscosityBiophysicsMolecular MedicineOsmotic pressureMolecular Biology

description

A survey is given of the most important methods for determination of molecular weight (osmotic pressure, ultracentrifuge and diffusion, light scattering, viscosity). All the methods require an extrapolation to zero concentration in order to use equations valid for ideal solutions. Furthermore the polymolecularity of the examined substance must be considered, since each method yields a characteristic average of molecular weight differing from the others. The present limits of the methods are given and their results compared.

https://doi.org/10.1007/bf02173733