6533b872fe1ef96bd12d38ac
RESEARCH PRODUCT
Sponge homologue to human and yeast gene encoding the longevity assurance polypeptide: differential expression in telomerase-positive and telomerase-negative cells of Suberites domuncula.
Werner E. G. M�llerRenate StefferRenato BatelHeinz C. SchröderMichael KruseIsabel M. Müllersubject
AgingTelomeraseDNA ComplementaryCell divisionCellGenes FungalMolecular Sequence DataGene ExpressionApoptosisBiologyYeastsGene expressionmedicineAnimalsHumansAmino Acid SequenceCloning MolecularGeneTelomeraseBase SequenceSequence Homology Amino AcidCell growthGene Expression ProfilingProteinsbiology.organism_classificationMolecular biologyPoriferaSuberites domunculaGene expression profilingmedicine.anatomical_structureBromodeoxyuridinePeptidesDevelopmental Biologydescription
Porifera show a characteristic Bauplan in spite of the fact that (almost) all cells are telomerase-positive and presumably provided with an unlimited potency for cell proliferation. Studies revealed that telomerase-positive cells can be triggered to telomerase-negative cells by dissociating them into single cells. Single cells from the demosponge Suberites domuncula, in contrast to cells present in primmorphs (a special form of cell aggregates), lack the property to proliferate and they undergo apoptosis. One gene, SDLAGL, was identified in primmorphs that showed high sequence similarity to the longevity assurance genes from other Metazoa. In single cells no transcripts of SDLAGL could be identified, while high expression was seen after re-aggregation of single cells and in proliferating cells of primmorphs. We concluded that SDLAGL is involved in the shift of telomerase-positive, proliferating cells to telomerase-negative, non-proliferating cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-09-28 | Mechanisms of ageing and development |