6533b873fe1ef96bd12d4aaa
RESEARCH PRODUCT
Flow-cytometric enumeration of reticulocytes with the new fluorochrome 1′,3′-diethyl-4,2′-quinolylthiacyanine
Rosa M. GuaschF. CarreteroG. JuanJosé-enrique O'connorsubject
Chromatographymedicine.diagnostic_testRoutine laboratoryHematologyGeneral MedicineBiologyThiazole orangeStainMolecular biologyFlow cytometrychemistry.chemical_compoundmedicine.anatomical_structurechemistryReticulocytemedicineEnumerationFluorescent stainBrilliant cresyl bluedescription
Several flow-cytometric methods for reticulocyte enumeration in whole blood have been developed, with different degrees of practical use. Recently, a new fluorochrome, 1′,3′-diethyl-4,2′-quinolylthyacianine (DEQTC) was proposed in a brief report, as an alternative to thiazole orange for reticulocyte counting. We have evaluated the usefulness of this fluorescent stain by assessing the optimal conditions for the flow-cytometric analysis, and by comparing in double-blind assays the quantitative results of this technique with those obtained by manual counting with brilliant cresyl blue. Our results show that flow cytometry with DEQTC is highly correlated to the manual method (r=0.95–0.99), supporting the interest of this particular stain and of flow cytometry for routine laboratory work in hematology.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-10-01 | Annals of Hematology |