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showing 10 items of 7219 documents

TRPM8 Channel Activation Reduces the Spontaneous Contractions in Human Distal Colon

2020

The transient receptor potential-melastatin 8 (TRPM8) is a non-selective Ca2+-permeable channel, activated by cold, membrane depolarization, and different cooling compounds. TRPM8 expression has been found in gut mucosal, submucosal, and muscular nerve endings. Although TRPM8 plays a role in pathological conditions, being involved in visceral pain and inflammation, the physiological functions in the digestive system remain unclear as yet. The aims of the present study were: (i) to verify the TRPM8 expression in human distal colon

0301 basic medicineMaleGene ExpressionPharmacologySettore BIO/09 - Fisiologialcsh:ChemistryTissue Culture Techniqueschemistry.chemical_compound0302 clinical medicineIntestinal MucosaReceptorlcsh:QH301-705.5Spectroscopyhuman colon contractilityAged 80 and overTetraethylammoniumDepolarizationGeneral MedicineIberiotoxinMiddle AgedComputer Science Applications030220 oncology & carcinogenesisTetrodotoxinFemaleMuscle ContractionAgonistSerotoninmedicine.drug_classColonTRPM Cation ChannelsTetrodotoxinApaminCatalysisArticleInorganic Chemistry03 medical and health sciencesIBSmedicineTRPM8HumansPhysical and Theoretical ChemistryMolecular BiologyAgedOrganic ChemistryMuscle SmoothTetraethylammonium chloridePhosphinic Acids1-[Diisopropyl-phosphinoyl]-alkane (DIPA)030104 developmental biologychemistrylcsh:Biology (General)lcsh:QD1-999ApaminTRPM-8PeptidesInternational Journal of Molecular Sciences
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Vitamin D Status and the Relationship with Bone Fragility Fractures in HIV-Infected Patients: A Case Control Study

2018

HIV-infected patients show high risk of fracture. The aims of our study were to determine the prevalence of vertebral fractures (VFs) and their associations with vitamin D in HIV patients. 100 patients with HIV infection and 100 healthy age-and sex-matched controls were studied. Bone mineral density was measured by quantitative ultrasound at the non-dominant heel. Serum osteocalcin and C-terminal telopeptide of collagen type 1 served as bone turnover markers. Bone ultrasound measurements were significantly lower in patients compared with controls (Stiffness Index (SI): 80.58 ± 19.95% vs. 93.80 ± 7.10%, respectively, p < 0.001). VFs were found in 16 patients and in 2 controls. HIV patie…

0301 basic medicineMaleHeelBone ultrasound; HIV; Osteoporosis; Vertebral fractures; Vitamin D; Catalysis; Molecular Biology; Spectroscopy; Computer Science Applications1707 Computer Vision and Pattern Recognition; Physical and Theoretical Chemistry; Organic Chemistry; Inorganic ChemistryOsteoporosisvitamin DHIV InfectionsGastroenterologyCatalysiBone remodelingFractures Bone0302 clinical medicineRisk Factors030212 general & internal medicineSpectroscopyBone mineralUltrasoundComputer Science Applications1707 Computer Vision and Pattern RecognitionPhosphorusGeneral MedicineMiddle AgedComputer Science Applicationsmedicine.anatomical_structureFemaleAdultmedicine.medical_specialtybone ultrasoundCatalysisArticleInorganic Chemistrybone ultrasound; HIV; osteoporosis; vertebral fractures; vitamin D03 medical and health sciencesN-terminal telopeptideInternal medicinemedicineVitamin D and neurologyHumansvertebral fracturesPhysical and Theoretical ChemistryMolecular Biologybusiness.industryOrganic ChemistryOsteoporosiCase-control studyHIVmedicine.diseaseosteoporosis030104 developmental biologyCase-Control StudiesCalciumVertebral fracturebusinessInternational Journal of Molecular Sciences
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Preventive Effect of Cow's Milk Fermented with Lactobacillus paracasei CBA L74 on Common Infectious Diseases in Children: A Multicenter Randomized Co…

2017

Background: Fermented foods have been proposed to prevent common infectious diseases (CIDs) in children attending day care or preschool. Objectives: To investigate the efficacy of dietary supplementation with cow’s skim milk fermented with the probiotic Lactobacillus paracasei CBA L74 in reducing CIDs in children attending day care or preschool. Methods: Multicenter, randomized, double-blind, placebo-controlled trial on healthy children (aged 12–48 months) consuming daily 7 grams of cow’s skim milk fermented with L. paracasei CBA L74 (group A), or placebo (maltodextrins group B) attending day care or preschool during the winter season. The main outcome was the proportion of children who exp…

0301 basic medicineMalePediatricsCultured Milk ProductsGroup Blaw.inventionDefensinsFeces0302 clinical medicineRandomized controlled triallawOtitisacute gastroenteritisinnate immunityRhinitisNutrition and DieteticsbiologyAbsolute risk reductionfood and beveragesPharyngitisLacticaseibacillus paracaseiGastroenteritisMilkChild Preschool030211 gastroenterology & hepatologyFemaleTracheitisprobioticacute gastroenteritimedicine.medical_specialtyLactobacillus paracaseiPlaceboCommunicable DiseasesArticle03 medical and health sciencesDouble-Blind MethodCathelicidinsInternal medicinemedicineAnimalsHumansimmunonutritionFecesIntention-to-treat analysisgut microbiotabusiness.industryProbioticsInfantacute gastroenteritis; upper respiratory tract infections; probiotics; innate immunity; acquired immunity; gut microbiota; immunonutritionupper respiratory tract infectionsbiology.organism_classificationmedicine.diseaseImmunoglobulin Aacquired immunity030104 developmental biologyUpper respiratory tract infectionupper respiratory tract infectionSample SizeFermentationCattlebusinessFood ScienceAntimicrobial Cationic Peptides
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Matching the Directions of Electric Fields from Triboelectric and Ferroelectric Charges in Nanogenerator Devices for Boosted Performance

2020

Summary Embedding additional ferroelectric dipoles in contacting polymer layers is known to enhance the performance of triboelectricnanogenerator (TENG) devices. However, the influence of dipoles formed between the triboelectric surface charges on two contacting ferroelectric films has been ignored in all relevant studies. We demonstrate that proper attention to the alignment of the distinct dipoles present between two contacting surfaces and in composite polymer/BaTiO3 ferroelectric films can lead to up to four times higher energy and power density output compared with cases when dipole arrangement is mismatched. For example, TENG device based on PVAc/BaTiO3 shows energy density increase f…

0301 basic medicineMaterials sciencePolymers02 engineering and technologyArticle03 medical and health sciencesElectric field:NATURAL SCIENCES:Physics [Research Subject Categories]DevicesNanotechnologySurface chargelcsh:ScienceTriboelectric effectPower densityMultidisciplinarybusiness.industryNanogeneratorElectrostatic induction021001 nanoscience & nanotechnologyFerroelectricityDipole030104 developmental biologyElectromagnetic Field13. Climate actionOptoelectronicsNanoparticleslcsh:Q0210 nano-technologybusinessiScience
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First phenotypic and genotypic description of Fasciola hepatica infecting highland cattle in the state of Mexico, Mexico

2018

Abstract Fascioliasis is a plantborne and zoonotic parasitic disease caused by fasciolid liver flukes. Fasciola hepatica is the only fasciolid species described in the Americas. Human fascioliasis endemic areas are mainly located in high altitude areas of the Americas. Given the necessity to characterize F. hepatica populations involved, the phenotypic and genotypic features of fasciolid adults infecting cattle in the highland area of Toluca, State of Mexico, Mexico, were analyzed and compared to fasciolid materials from the Northern Bolivian Altiplano, representing the altiplanic transmission pattern in a hyperendemic scenario. A computer image analysis system (CIAS) was applied on the bas…

0301 basic medicineMicrobiology (medical)FascioliasisGenotypeRange (biology)030231 tropical medicinebiology.animal_breedPopulationCattle DiseasesZoologyDNA RibosomalMicrobiology03 medical and health sciences0302 clinical medicineIntergenic regionHepaticaparasitic diseasesGeneticsAnimalsFasciola hepaticaGeography MedicaleducationMexicoMolecular BiologyRibosomal DNAEcology Evolution Behavior and SystematicsBase Compositioneducation.field_of_studybiologyHighland CattleFasciola hepatica030108 mycology & parasitologyLiver flukebiology.organism_classificationPhenotypeInfectious DiseasesHaplotypesCattleSequence AnalysisInfection, Genetics and Evolution
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Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases

2020

13 páginas, 6 figuras, 3 tablas

0301 basic medicineModels MolecularBioquímicaHistidine KinaseProtein ConformationScience030106 microbiologyPhosphataseGeneral Physics and AstronomyMicrobiologiaCrystallography X-RayModels BiologicalBiochemistryMicrobiologyGeneral Biochemistry Genetics and Molecular BiologyCatalysisArticleEnzyme catalysis03 medical and health sciencesResidue (chemistry)Protein structureBacterial ProteinsMultienzyme ComplexesHistidineThermotoga maritimaPhosphorylationlcsh:ScienceAuthor CorrectionHistidineX-ray crystallographyMultidisciplinaryEffectorChemistryEscherichia coli ProteinsQGeneral ChemistryHydrogen-Ion ConcentrationResponse regulator030104 developmental biologyBiochemistryMutationTrans-ActivatorsPhosphorylationlcsh:QBacterial Outer Membrane Proteins
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Prefolded Synthetic G-Quartets Display Enhanced Bioinspired Properties

2016

International audience; A water-soluble template-assembled synthetic G-quartet (TASQ) based on the use of a macrocyclodecapeptide scaffold was designed to display stable intramolecular folds alone in solution. The preformation of the guanine quartet, demonstrated by NMR and CD investigations, results in enhanced peroxidase-type biocatalytic activities and improved quadruplex-interacting properties. Comparison of its DNAzyme-boosting properties with the ones of previously published TASQ revealed that, nowadays, it is the best DNAzyme-boosting agent.

0301 basic medicineModels MolecularGuanineStereochemistryDNAzymewaterSupramolecular chemistryDeoxyribozymednainsights010402 general chemistryG-QuartetsG-quadruplexchemistry[ CHIM ] Chemical Sciences01 natural sciencesCatalysissupramolecular chemistryg-quadruplex structures03 medical and health scienceschemistry.chemical_compoundG-quartets[CHIM]Chemical SciencesrnaComputingMilieux_MISCELLANEOUSligandsbiologyOrganic Chemistry[CHIM.CATA]Chemical Sciences/CatalysisGeneral ChemistryDNA CatalyticSmall moleculeG-quadruplexes0104 chemical sciencesSolutionssmall molecules030104 developmental biologychemistryBiocatalysisIntramolecular forceBiocatalysisNucleic Acid Conformationcyclodecapeptideacid
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Usefulness of current candidate genetic markers to identify childhood cancer patients at risk for platinum-induced ototoxicity: Results of the Europe…

2020

Background Irreversible sensorineural hearing loss is a common side effect of platinum treatment with the potential to significantly impair the neurocognitive, social and educational development of childhood cancer survivors. Genetic association studies suggest a genetic predisposition for cisplatin-induced ototoxicity. Among other candidate genes, thiopurine methyltransferase (TPMT) is considered a critical gene for susceptibility to cisplatin-induced hearing loss in the FDA drug label and a pharmacogenetic guideline. The aim of this cross-sectional cohort study was to confirm the genetic associations in a large pan-European population and to evaluate the diagnostic accuracy of the genetic…

0301 basic medicineOncologyMaleCancer ResearchCandidate genePharmacogenomic VariantsCancer survivorsCHILDRENAnti-neoplastic drugsVARIANTSOCT2Carboplatin0302 clinical medicineHearingRisk FactorsNeoplasmsTPMTHearing / drug effectsProspective StudiesAge of OnsetChild610 Medicine & healthPREDICTORSmedia_commonHearing Loss Sensorineural / physiopathologyeducation.field_of_studyddc:618Thiopurine methyltransferasebiologycarboplatin [Cisplatin]Neoplasms / drug therapyOrganic Cation Transporter 2EuropeOncologyCisplatin: carboplatinCisplatin / adverse effects030220 oncology & carcinogenesisChild PreschoolOrganic Cation Transporter 2 / geneticsFemaleSENSITIVITYChildhood cancer360 Social problems & social servicesCohort studyDrug-induced ototoxicitymedicine.medical_specialtyINDUCED HEARING-LOSSAdolescentMulticenter cohort studyHearing Loss SensorineuralPopulationAdverse drug reactionAntineoplastic AgentsPolymorphism Single NucleotideRisk AssessmentHearing Loss Sensorineural / chemically inducedCarboplatin / adverse effects03 medical and health sciencesACYP2OtotoxicitySDG 3 - Good Health and Well-beingInternal medicinemedicineGenetic predispositionmedia_common.cataloged_instanceHumansGenetic Predisposition to DiseaseCISPLATIN-INDUCED OTOTOXICITYEuropean unioneducationGenetic Association StudiesGenetic associationRetrospective Studiesbusiness.industryAntineoplastic Agents / adverse effectsInfant NewbornInfantOdds ratioGuidelinemedicine.diseaseOtotoxicityCOMTPharmacogenomic Testing030104 developmental biologyCross-Sectional StudiesPharmacogeneticsbiology.proteinGenetic markersHearing Loss Sensorineural / geneticsCisplatinbusinessPharmacogenetics
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Associations of Pathogenic Variants in MLH1, MSH2, and MSH6 With Risk of Colorectal Adenomas and Tumors and With Somatic Mutations in Patients With L…

2020

Contains fulltext : 220040.pdf (Publisher’s version ) (Closed access) BACKGROUND & AIMS: Lynch syndrome is caused by variants in DNA mismatch repair (MMR) genes and associated with an increased risk of colorectal cancer (CRC). In patients with Lynch syndrome, CRCs can develop via different pathways. We studied associations between Lynch syndrome-associated variants in MMR genes and risks of adenoma and CRC and somatic mutations in APC and CTNNB1 in tumors in an international cohort of patients. METHODS: We combined clinical and molecular data from 3 studies. We obtained clinical data from 2747 patients with Lynch syndrome associated with variants in MLH1, MSH2, or MSH6 from Germany, the Net…

0301 basic medicineOncologyMaleColorectal cancerDNA Mutational Analysisgenetic analysisHEREDITARYcancer riskGUIDELINESDNA Mismatch Repair0302 clinical medicineGermanyTumours of the digestive tract Radboud Institute for Molecular Life Sciences [Radboudumc 14]Prospective Studiesprognostic factorFinlandbeta CateninNetherlandsOutcomePrognostic FactorGastroenterologyGenetic AnalysisColonoscopyMiddle AgedCANCERLynch syndromeCancer Risk3. Good healthDNA-Binding ProteinsDEFICIENCYMutS Homolog 2 Proteinsyöpägeenitoutcome030211 gastroenterology & hepatologyDNA mismatch repairFemaleMutL Protein Homolog 1geenitutkimusAdenomaAdultmedicine.medical_specialtycongenital hereditary and neonatal diseases and abnormalitiesAdenoma3122 CancersAdenomatous Polyposis Coli ProteinINSTABILITYSOCIETYMLH103 medical and health sciencesInternal medicinemedicineMANAGEMENTHumansLynchin oireyhtymäneoplasmspaksusuolisyöpäHepatologybusiness.industryCancernutritional and metabolic diseasesennusteetmedicine.diseaseColorectal Neoplasms Hereditary Nonpolyposisdigestive system diseasesMSH6030104 developmental biologyMSH2Mutationbusiness
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Tetraspanin CD63 controls basolateral sorting of organic cation transporter 2 in renal proximal tubules.

2016

CD63 is a ubiquitously expressed member of the tetraspanin superfamily. Using a mating-based split-ubiquitin-yeast 2-hybrid system, pull-down experiments, total internal reflection fluorescence microscopy, Forster resonance energy transfer, and biotinylation assays, we found that CD63 interacts with human organic cation transporter 2 (hOCT2), which transports endogenous and exogenous substrates, such as neurotransmitters and drugs in several epithelial cells. CD63 overexpression affects cellular localization of hOCT2 expressed in human embryonic kidney (HEK)293 cells. Studies with CD63-knockout mice indicate that in renal proximal tubules, CD63 determines the insertion of the mouse ortholog…

0301 basic medicineOrganic Cation Transport ProteinsEndosomeEndosomesBiochemistryMadin Darby Canine Kidney CellsKidney Tubules Proximal03 medical and health sciencesMiceDogsTetraspaninGeneticsAnimalsHumansMolecular BiologyCellular localizationEpithelial polarityChemistryTetraspanin 30rab4 GTP-Binding ProteinsHEK 293 cellsCell MembraneOrganic Cation Transporter 2TransporterEpithelial CellsTransfectionCell biologyMice Inbred C57BLProtein Transport030104 developmental biologyHEK293 CellsMembrane proteinBiotechnologyProtein BindingFASEB journal : official publication of the Federation of American Societies for Experimental Biology
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