Search results for " Competitive"
showing 10 items of 237 documents
Psychotropic drug competition for [3H]imipramine binding further indicates the presence of only one high-affinity drug binding site on human α1-acid …
1983
Fatty acids bind to the fungal elicitor cryptogein and compete with sterols
2001
Abstract Cryptogein is a proteinaceous elicitor of plant defense reactions which also exhibits sterol carrier properties. In this study, we report that this protein binds fatty acids. The stoichiometry of the fatty acid–cryptogein complex is 1:1. Linoleic acid and dehydroergosterol compete for the same site, but elicitin affinity is 27 times lower for fatty acid than for sterol. We show that C7 to C12 saturated and C16 to C22 unsaturated fatty acids are the best ligands. The presence of double bonds markedly increases the affinity of cryptogein for fatty acids. A comparison between elicitins and known lipid transfer proteins is discussed.
l-Tyrosine β-naphthylamide is a potent competitive inhibitor of tyramine N-(hydroxycinnamoyl)transferase in vitro
2001
L-Tyrosine beta-naphthylamide, a synthetic substrate designed to measure tyrosine aminopeptidase activity, is a potent inhibitor of hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase (THT) purified from elicited tobacco cell-suspension cultures. The inhibition is competitive, with the inhibitor binding reversibly to the tyramine binding site of the enzyme. Similar results were obtained with THT extracted from elicited potato cell-suspension cultures. Ki values were found to be 0.66 microM for the enzyme from tobacco and 0.3 microM for the enzyme from potato. L-Tyrosine 7-amido-4-methylcoumarin, a fluorogenic substrate for tyrosine aminopeptidases, the structure of which is close …
Faktori, kas ietekmē Latvijas piena nozares konkurētspēju Baltijas valstīs
2019
This study presents competitiveness analysis and factors influencing on the competitiveness in Latvian dairy sector among the Baltic states. Gross domestic product, the number of population, demographics, trade balance, labour cost indicators of each country and numbers of manufacturers, the revenue of the sector and its growth rate, volume sold of goods and its growth rate, average volume sold per capita of products, an average price of milk and cream, an average price of yoghurt and curdled milk, an average price of cream cheese and cheese in the dairy sector of every Baltic state are compared between among them and has been commented by three experts: Inga Orlova ( representative of Mini…
Measuring International Competitiveness of Romania by Using Porter's Diamond and Revealed Comparative Advantage
2013
Abstract International competitiveness generate a number of challenges because creates profitability for companies, welfare for citizens, sustainable prosperity for the economy as long as it is perceived as a constructive concept. The aim of present paper is to identify the international competitiveness of Romania by analyze the competitive advantage and disadvantage according to Porter‘s Diamond, on one hand, and by calculate revealed comparative advantage according to Balasa, on the other hand. The results show that Romanian has more competitive disadvantage than competitive advantage, even if by comparison with European Union, Romania has some revealed comparative advantage.
Interaction of 68–kDa TAR RNA-binding protein and other cellular proteins with rpion protein-RNA stem-loop
1995
The RNA stem-loop structure of the trans-activating region TAR sequence of human immunodeficiency virus-1 mRNA is the binding site for a number of host cell proteins. A virtually identical set of proteins from HeLa nuclear extracts was found to bind to the predicted RNA hairpin element of prion protein (PrP) mRNA, as demonstrated in UV cross-linking/RNase protection and Northwestern assays. We show that the cellular TAR loop-binding protein, p68, is among those proteins which associate with PrP RNA. Competition experiments with various TAR RNA mutants revealed that binding of partially purified p68 to PrP RNA stem-loop occurs sequence-specifically. The 100-kDa 2-5A synthetase which is invol…
Functional characterization of two melanocortin (MC) receptors in lamprey showing orthology to the MC1 and MC4 receptor subtypes
2007
Abstract Background The melanocortin (MC) receptors have a key role in regulating body weight and pigmentation. They belong to the rhodopsin family of G protein-coupled receptors (GPCRs). The purpose of this study was to identify ancestral MC receptors in agnathan, river lamprey. Results We report cloning of two MC receptors from river lamprey. The lamprey receptors, designated MCa and MCb, showed orthology to the MC1 and MC4 receptor subtypes, respectively. The molecular clock analysis suggested that lamprey MC receptor genes were not duplicated recently and diverged from each other more than 400 MYR ago. Expression and pharmacological characterization showed that the lamprey MCa receptor …
Designing dynamic performance management systems to foster SME competitiveness according to a sustainable development perspective: empirical evidence…
2015
The role of performance management (PM) systems has become crucial for steering small-medium enterprises (SMEs) to successfully compete during the ongoing critical economic transition. To improve decision-maker strategic learning processes, traditional PM frameworks need to be combined with system dynamics (SD) modelling. This paper shows how to design and use a dynamic performance management (DPM) approach to assess and support SMEs competitiveness according to a sustainable development perspective. The emerging framework is applied to a real case of an Italian small business to analyse the empirical effectiveness of the approach hereby suggested.
Role of toxin activation on binding and pore formation activity of the Bacillus thuringiensis Cry3 toxins in membranes of Leptinotarsa decemlineata (…
2004
AbstractBinding and pore formation constitute key steps in the mode of action of Bacillus thuringiensis δ-endotoxins.In this work, we present a comparative analysis of toxin-binding capacities of proteolytically processed Cry3A, Cry3B and Cry3C toxins to brush border membranes (BBMV) of the Colorado potato beetle Leptinotarsa decemlineata (CPB), a major potato coleopteran-insect pest. Competition experiments showed that the three Cry3 proteolytically activated toxins share a common binding site. Also heterologous competition experiments showed that Cry3Aa and Cry3Ca toxins have an extra binding site that is not shared with Cry3Ba toxin. The pore formation activity of the three different Cry…
α1-Adrenoceptors in the rat cerebral cortex: New insights into the characterization of α1L- and α1D-adrenoceptors
2010
36 p., figuras y tablas, bibliografía