Search results for " Confocal Microscopy"
showing 7 items of 17 documents
In-vivo-Konfokalmikroskopie in der Diagnose des okulären Schleimhautpemphigoids
2015
Hintergrund: Fur die Prognose des okularen Schleimhautpemphigoids (mucous membrane pemphigoid, MMP) ist eine fruhe Diagnosesicherung essenziell. Allerdings wird die Sensitivitat des sog. diagnostischen Goldstandards, der direkten (DIF) bzw. indirekten Immunfluoreszenz (IIF) mit einer Spanne von 30–80 % sehr variabel angegeben. Zusatzlich birgt die dafur notwendige Bindehautbiopsie die Gefahr der Exazerbation. Ziel dieser Studie war es, zu ermitteln, ob die nicht invasive In-vivo-Konfokalmikroskopie zur Erkennung eines okularen Schleimhautpemphigoids beitragen kann. Patienten und Methoden: Die Konjunktiva von 10 Pemphigoidpatienten und 10 Kontrollprobanden wurden mithilfe des o. g. Verfahren…
Uptake of silica covered Quantum Dots into living cells: Long term vitality and morphology study on hyaluronic acid biomaterials
2015
Quantum Dots (QDs) are promising very bright and stable fluorescent probes for optical studies in the biological field but water solubility and possible metal bio-contamination need to be addressed. In this work, a simple silica-QD hybrid system is prepared and the uptake in bovine chondrocytes living cells without any functionalization of the external protective silica shield is demonstrated. Moreover, long term treated cells vitality (up to 14 days) and the transfer of silica-QDs to the next cell generations are here reported. Confocal fluorescence microscopy was also used to determine the morphology of the so labelled cells and the relative silica-QDs distribution. Finally, we employ sil…
Natural autofluorescence in archeological bone
2017
The human bone present a natural autofluorescence which is tipically induced by natural antibiotiscs absorbed by collagen and in part is due to phenomena not weel know and referred to an enigmatic endogenous fluophore. Is iintristing for us to see the autofluorescence in the various age of the observed samples.
Biocompatibility and staining properties of CdSe\CdS\SiO2 nanoparticles for in-vitro biomaterials characterization
2015
Semiconductor nanocrystals, known as quantum dots (QDs) display well-tuned emission spectra from ultraviolet to the infrared region. Their photostability is greatly enhanced compared to fluorophores making them suitable for optical bio-imaging. In this work we have studied the cytocompatibility of CdSe\CdS\SiO2 QDs towards bovine articular chondrocytes in order to establish if these nanoparticles could be used as staining system forcells in tissue engineering purposes. Studies of QDs uptake and cell viability were conducted through morphology evaluation and MTS test. The adhesion behavior of chondrocytes deposited onto scaffolds of a derivative of hyaluronic acid functionalized with octadec…
Reflectance mode confocal microscopy and digital image analysis in naevus of Hori and pathogenetic evaluation
2012
Extraction et évaluation de caractéristiques adaptées pour la classification du Lentigo à partir d’images de Microscopie Confocale
2019
International audience; La détection de cancer de la peau est l’un des défis de ces dernières décennies. Par ailleurs, diverses techniques d’imagerie ont pour objectif d’aider à la reconnaissance de ces pathologies malignes en contexte clinique. La Microscopie Confocale par Réflectance est un exemple de technique d’imagerie adaptée à la détection de maladie de la peau sur laquelle nous nous basons pour la détection de Lentigo. Les travaux présentés dans cet article portent sur la classification de ces images en trois catégories : sain, bénin et malin. Dans ce but, nous proposons et évaluons deux méthodes d’extraction de caractéristiques basées sur les descripteurs d’Haralick pour l’une et s…