Search results for " DOM"

showing 10 items of 2750 documents

A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase

1999

Protein tyrosine phosphatase PTP-SL retains mitogen-activated protein (MAP) kinases in the cytoplasm in an inactive form by association through a kinase interaction motif (KIM) and tyrosine dephosphorylation. The related tyrosine phosphatases PTP-SL and STEP were phosphorylated by the cAMP-dependent protein kinase A (PKA). The PKA phosphorylation site on PTP-SL was identified as the Ser231 residue, located within the KIM. Upon phosphorylation of Ser231, PTP-SL binding and tyrosine dephosphorylation of the MAP kinases extracellular signal–regulated kinase (ERK)1/2 and p38α were impaired. Furthermore, treatment of COS-7 cells with PKA activators, or overexpression of the Cα catalytic subunit …

Cytoplasmanimal structuresRecombinant Fusion ProteinsCèl·lulesAmino Acid MotifsNerve Tissue ProteinsProtein tyrosine phosphataseSH2 domainTransfectionenvironment and public healthModels Biologicalp38 Mitogen-Activated Protein KinasesReceptor tyrosine kinaseSH3 domainCell LinePhosphoserinetyrosine phosphatasesAnimalsHumansProtein phosphorylationPKAReceptor-Like Protein Tyrosine Phosphatases Class 7PhosphorylationPTP-SLCell NucleusMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3biologyBrief ReportIntracellular Signaling Peptides and ProteinsBiological TransportCell BiologyProtein Tyrosine Phosphatases Non-ReceptorCyclic AMP-Dependent Protein KinasesEnzyme Activationenzymes and coenzymes (carbohydrates)MAP kinasesBiochemistryMitogen-activated protein kinaseCOS CellsMutationbiology.proteinPhosphorylationMitogen-Activated Protein KinasesProtein Tyrosine PhosphatasesEnzimssignal transductionProto-oncogene tyrosine-protein kinase Src
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Immunosurveillance of lung melanoma metastasis in EBI-3-deficient mice mediated by CD8+ T cells.

2008

Abstract EBV-induced gene 3 (EBI-3) codes for a soluble type I receptor homologous to the p40 subunit of IL-12 that is expressed by APCs following activation. In this study, we assessed the role of EBI-3 in a model of lung melanoma metastasis. Intravenous injection of the B16-F10 cell line resulted in a significant reduction of lung tumor metastasis in EBI-3−/− recipient mice compared with wild-type mice. The immunological finding accompanying this effect was the expansion of a newly described cell subset called IFN-γ producing killer dendritic cells associated with CD8+ T cell responses in the lung of EBI-3−/− mice including IFN-γ release and TNF-α-induced programmed tumor cell death. Depl…

Cytotoxicity ImmunologicAdoptive cell transferLung NeoplasmsT cellImmunologyMelanoma ExperimentalBiologyCD8-Positive T-LymphocytesArticleMetastasisMinor Histocompatibility AntigensGene Knockout TechniquesMiceCell Line TumormedicineImmunology and AllergyCytotoxic T cellAnimalsLung MelanomaReceptors CytokineImmunologic SurveillanceCell Line TransformedMice KnockoutMelanomamedicine.diseaseImmunosurveillanceMice Inbred C57BLmedicine.anatomical_structureCell Transformation NeoplasticImmunologyInjections IntravenousAnimals; CD8-Positive T-Lymphocytes; Cell Line Transformed; Cell Line Tumor; Cell Transformation Neoplastic; Cytotoxicity Immunologic; Gene Knockout Techniques; Immunologic Surveillance; Injections Intravenous; Lung Neoplasms; Melanoma Experimental; Mice; Mice Inbred C57BL; Mice Knockout; Neoplasm Transplantation; Receptors Cytokine; T-Box Domain ProteinsCancer researchT-Box Domain ProteinsCD8Neoplasm Transplantation
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Targeting positive regulatory domain I-binding factor 1 and X box-binding protein 1 transcription factors by multiple myeloma-reactive CTL.

2005

Abstract Growing evidence indicates that multiple myeloma (MM) and other malignancies are susceptible to CTL-based immune interventions. We studied whether transcription factors inherently involved in the terminal differentiation of mature B lymphocytes into malignant and nonmalignant plasma cells provide MM-associated CTL epitopes. HLA-A*0201 (A2.1) transgenic mice were used to identify A2.1-presented peptide Ag derived from the plasma cell-associated transcriptional regulators, positive regulatory domain I-binding factor 1 (PRDI-BF1) and X box-binding protein 1 (XBP-1). A2.1-restricted CTL specific for PRDI-BF1 and XBP-1 epitopes efficiently killed a variety of MM targets. PRDI-BF1- and X…

Cytotoxicity ImmunologicX-Box Binding Protein 1Cellular differentiationImmunologyEpitopes T-LymphocyteMice TransgenicRegulatory Factor X Transcription FactorsBiologyEpitopeMiceImmune systemCell Line TumorHLA-A2 AntigenImmunology and AllergyAnimalsHumansTranscription factorAntigen PresentationB-LymphocytesCell DeathT-cell receptorCell DifferentiationCytotoxicity Tests ImmunologicX-Box Binding Protein 1Molecular biologyPeptide FragmentsCell biologyDNA-Binding ProteinsMice Inbred C57BLRepressor ProteinsCTL*Self ToleranceNIH 3T3 CellsPositive Regulatory Domain I-Binding Factor 1Multiple MyelomaCD8T-Lymphocytes CytotoxicTranscription FactorsJournal of immunology (Baltimore, Md. : 1950)
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Gestācijas dominantes psiholoģiskās komponentes hipogestognostiskā varianta izplatība un saistība ar atioforiognoziju dzemdību palīdzības iestādes pa…

2015

Ievads. Gestācijas dominantes psiholoģiskā komponente ir psihisko pašregulācijas mehānismu kopums grūtniecības saglabāšanai. Hipogestognostiskais variants raksturīgs sievietēm, kuras apzināti vai biežāk neapzināti nepieņem savu grūtniecību. Sakarā ar to, ka gestācijas dominantes psiholoģiskās komponentes hipogestognostiskais variants ir cieši saistīts ar grūtniecības noliegšanu, kas apdraud gan mātes, gan bērna dzīvību, nepieciešams pēc iespējas agrīni novērtēt grūtnieces psiholoģisko stāvokli. Pētījuma mērķis. Veicot prospektīvo pētījumu novērtēt grūtnieču gestācijas dominantes psiholoģiskās komponentes hipogestognostiskā varianta biežumu, kā arī noskaidrot grūtniecības nelabvēlīga iznākum…

DCMGestācijas dominantes psiholoģiskās komponentes hipogestognostiskais variantsSSKAtiogoriognozijaGrūtnieču attieksmju tests / Dobrjakova anketaMedicīna
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L' équipement automobile et les habitudes de déplacement domicile-travail dans l'espace français (hors outre-mer) selon le Recensement entr…

2020

Colloque : Peut-on se passer de la voiture hors des centres urbains ?, Guyancourt, FRANCE, 27-/02/2020 - 27/02/2020; Selon les recensements récents de la population (RP), la très grande majorité des actifs déclarent aller travailler en voiture. Cette source permet de discuter, pour la période 2006-2016, l'affirmation selon laquelle la voiture serait toujours plus indispensable hors des grands centres urbains où, au contraire, son utilisation et sa possession stagneraient. Le recensement a ses limites, mais il permet de cartographier finement les pratiques dans tout le pays et de les analyser selon des typologies géographiques adaptées aux problématiques du transport. Il permet aussi de…

DENSITE[SHS.SOCIO]Humanities and Social Sciences/Sociology[SHS.SOCIO] Humanities and Social Sciences/SociologyTRAJET DOMICILE TRAVAILRECENSEMENTAUTOMOBILE
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Sex-specific windows for high mRNA expression of DNA methyltransferases 1 and 3A and methyl-CpG-binding domain proteins 2 and 4 in human fetal gonads

2006

DNA methyltransferases (DNMTs) and 5-methyl-CpG-binding domain proteins (MBDs) are involved in the acquisition of parent-specific epigenetic modifications in human male and female germ cells. Reverse Northern blot analyses demonstrated sex-specific differences in mRNA expression for the maintenance DNMT1 and the de novo DNMT3A in developing testis and ovary. In fetal testis DNMT1 and DNMT3A expression peaked in mitotically arrested spermatogonia around 21 weeks gestation. In fetal ovary transcriptional upregulation of DNMT1 and DNMT3A occurred during a very brief period at 16 weeks gestation, when the oocytes proceeded through meiotic prophase. Fetal gonads showed several fold higher DNMT3A…

DNA (Cytosine-5-)-Methyltransferase 1MaleMethyltransferaseEmbryonic DevelopmentGestational AgeOvaryBiologyGene Expression Regulation EnzymologicChromatin remodelingDNA Methyltransferase 3AFetal DevelopmentPregnancyTestisGeneticsmedicineHumansDNA (Cytosine-5-)-MethyltransferasesRNA MessengerEpigeneticsRegulation of gene expressionFetusReverse Transcriptase Polymerase Chain Reactionurogenital systemOvaryGene Expression Regulation DevelopmentalCell BiologyReverse northern blotMolecular biologyMethyl-CpG-binding domainCell biologyDNA-Binding Proteinsmedicine.anatomical_structureembryonic structuresFemaleTranscription FactorsDevelopmental BiologyMolecular Reproduction and Development
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Establishment and functional validation of a structural homology model for human DNA methyltransferase 1

2003

Changes in DNA methylation patterns play an important role in tumorigenesis. The DNA methyltransferase 1 (DNMT1) protein represents a major DNA methyltransferase activity in human cells and is therefore a prominent target for experimental cancer therapies. However, there are only few available inhibitors and their high toxicity and low specificity have so far precluded their broad use in chemotherapy. Based on the strong conservation of catalytic DNA methyltransferase domains we have used a homology modeling approach to determine the three-dimensional structure of the DNMT1 catalytic domain. Our results suggest an overall structural conservation with other DNA methyltransferases but also in…

DNA (Cytosine-5-)-Methyltransferase 1Models MolecularMethyltransferaseMolecular Sequence DataBiophysicsDNA Methyltransferase InhibitorComputational biologyBiologymedicine.disease_causeModels BiologicalBiochemistryDNA methyltransferasechemistry.chemical_compoundCatalytic DomainTumor Cells CulturedmedicineHumansAmino Acid SequenceDNA (Cytosine-5-)-MethyltransferasesHomology modelingEnzyme InhibitorsMolecular BiologyGeneticsSequence Homology Amino AcidCell BiologyDNA MethylationModels ChemicalchemistryDNA methylationAzacitidineDNMT1Nucleic Acid ConformationCarcinogenesisDNABiochemical and Biophysical Research Communications
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Deinococcus radiodurans' SRA-HNH domain containing protein Shp (Dr1533) is involved in faithful genome inheritance maintenance following DNA damage

2018

WOS:000452343100012; International audience; Background: Deinococcus radiodurans R1 (DR) survives conditions of extreme desiccation, irradiation and exposure to genotoxic chemicals, due to efficient DNA breaks repair, also through Mn2+ protection of DNA repair enzymes. Methods: Possible annotated domains of the DR1533 locus protein (Shp) were searched by bioinformatic analysis. The gene was cloned and expressed as fusion protein. Band-shift assays of Shp or the SRA and HNH domains were performed on oligonucleotides, genomic DNA from E. coif and DR. slip knock-out mutant was generated by homologous recombination with a kanamycin resistance cassette. Results: DR1533 contains an N-terminal SRA…

DNA RepairDNA cytosine-methylation; DNA damage; DR1533 locus; Genotoxic agents; Mn2+; SRA domain; Biophysics; Biochemistry; Molecular BiologyGenotoxic agents[SDV]Life Sciences [q-bio]DNA cytosine-methylationperspectiveSettore BIO/19 - Microbiologia GeneraleBiochemistrychemistry.chemical_compound0302 clinical medicineKanamycinCloning Molecularcytosine0303 health sciencesDR1533 locusbiologyChemistryGenotoxic agentuhrf1Mn(2+)Mn2+SRA domainDeinococcusrecognitionmanganese(ii)DNA BacterialDNA damageDNA repairoxidationUbiquitin-Protein LigasesBiophysicsSettore BIO/11 - Biologia Molecolareresistance03 medical and health sciencesBacterial ProteinsProtein DomainsDR1533 locuDrug Resistance BacterialEscherichia coliHumansfeaturesAmino Acid SequenceGeneMolecular Biology030304 developmental biologyOligonucleotideComputational BiologyDeinococcus radioduransDNA Methylationbiology.organism_classificationMolecular biologygenomic DNArepairMutationCCAAT-Enhancer-Binding ProteinsDNA damageHomologous recombination030217 neurology & neurosurgeryDNAGenome BacterialMutagens
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A neutralizing antibody against human DNA polymerase epsilon inhibits cellular but not SV40 DNA replication.

1999

The contribution of human DNA polymerase epsilon to nuclear DNA replication was studied. Antibody K18 that specifically inhibits DNA polymerase activity of human DNA polymerase epsilon in vitro significantly inhibits DNA synthesis both when microinjected into nuclei of exponentially growing human fibroblasts and in isolated HeLa cell nuclei. The capability of this neutralizing antibody to inhibit DNA synthesis in cells is comparable to that of monoclonal antibody SJK-132-20 against DNA polymerase alpha. Contrary to the antibody against DNA polymerase alpha, antibody K18 against DNA polymerase epsilon did not inhibit SV40 DNA replication in vitro. These results indicate that DNA polymerase e…

DNA ReplicationDNA polymeraseDNA polymerase IIDNA polymerase epsilonSimian virus 40Virus ReplicationDNA polymerase deltaAntibodiesCell LineNeutralization TestsCatalytic DomainGeneticsAnimalsHumansPolymeraseDNA clampbiologyDNA replicationDNA Polymerase IIFibroblastsMolecular biologyProliferating cell nuclear antigenBromodeoxyuridineDNA Viralbiology.proteinCattleRabbitsHeLa CellsResearch ArticleNucleic acids research
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Origin of the integrin-mediated signal transduction. Functional studies with cell cultures from the sponge Suberites domuncula

1999

Sponges (phylum Porifera) represent the phylogenetically oldest metazoan animals. Recently, from the marine sponge Geodia cydonium a first cDNA encoding a putative integrin receptor molecule was isolated. In the present study basic functional experiments have been conducted to test the hypothesis that in sponges integrin polypeptides also function as adhesion molecules and as outside-in signaling molecules. The sponge Suberites domuncula has been used for the experiments because from this sponge only has a cell culture been established. Here we report that aggregation factor (AF)-mediated cell-cell adhesion is blocked by the RGDS peptide which is known to interact with beta integrin. Both R…

DNA ReplicationIntegrinsMolecular Sequence DataIntegrinBiologyBiochemistryCD49cEvolution MolecularCalmodulinCell AdhesionAnimalsAmino Acid SequenceRNA MessengerCloning MolecularCell adhesionCells CulturedCell AggregationCell adhesion moleculeSequence Analysis DNAbiology.organism_classificationCell aggregationPoriferaCell biologysuberites domuncula; integrin; calcium; ras; calmodulin; signal transduction; evolution; rgd(s)Suberites domunculaGene Expression RegulationIntegrin alpha Mras Proteinsbiology.proteinCalciumIntegrin beta 6Cell Adhesion MoleculesOligopeptidesSignal TransductionEuropean Journal of Biochemistry
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