Search results for " EXPERIMENTAL"

Inactivation of the KSRP gene modifies collagen antibody induced arthritis.

2017

Abstract The KH type splicing regulatory protein (KSRP) is a nucleic acid binding protein, which negatively regulates the stability and/or translatability of many mRNA species encoding immune-relevant proteins. As KSRP is expressed in immune cells including T and B cells, neutrophils, macrophages and dendritic cells, we wanted to analyze its importance for the development of autoimmune diseases. We chose collagen antibody-induced arthritis (CAIA) as an appropriate autoimmune disease mouse model in which neutrophils and macrophages constitute the main effector cell populations. We compared arthritis induction in wild type (WT) and KSRP−/− mice and paws were taken for histological sections an…

Identification of metastasis-related genes by genomic and transcriptomic studies in murine melanoma.

2021

Abstract Aims We systematically characterized metastatic murine B16-F10 melanoma, a sub-line derived from murine melanoma B16-F1 cells. Materials and methods RNA-sequencing and network analyses (Ingenuity Pathway Analysis) were performed to identify novel potential metastasis mechanisms. Chromosomal aberrations were identified by multicolor fluorescence in situ hybridization (mFISH) using all 21 murine whole chromosome painting probes. Key findings Numerous genes were overexpressed in B16-F10 cells, some of which have been already described as being metastasis-linked. Nr5a1/sf1, a known prognostic marker for adrenal tumors, was 177-fold upregulated in B16-F10 cells compared to B16-F1 cells.…

Influence of platelet-derived growth factor on osseous remodeling properties of a variable-thread tapered dental implant in vivo

2016

Objectives To evaluate the effect of platelet-derived growth factor (rhPDGF-BB) on the promotion of osteogenesis around variable-thread tapered implants in an animal model. Material and methods Twenty-four variable-thread tapered implants were inserted in the tibia of 12 rabbits. Twelve sites received additional rhPDGF-BB released from a presoaked xenogenic bone block that was fixed supracrestally. Primary outcomes were bone-to-implant contact (BIC; in % ± SD) and percentage of medullary bone fill around the implants (PMF; in % ± SD) after 3 weeks (PDGF n = 6, no PDGF n = 6) and 6 weeks (PDGF n = 6, no PDGF n = 6). Results Considerable crestal and medullary bone remodeling could be found ar…

Tissue microenvironment dictates the fate and tumor-suppressive function of type 3 ILCs

2017

Nussbaum et al. found that tumor suppression through innate lymphoid cells (ILCs) cannot be predicted solely based on the ILC phenotype and lineage but that their immune properties are shaped both by their ontogeny and by the tissue microenvironment they reside in.

Ca2+ signalling plays a role in celastrol‐mediated suppression of synovial fibroblasts of rheumatoid arthritis patients and experimental arthritis in…

2019

Background and purpose Celastrol exhibits anti-arthritic effects in rheumatoid arthritis (RA), but the role of celastrol-mediated Ca2+ mobilization in treatment of RA remains undefined. Here, we describe a regulatory role for celastrol-induced Ca2+ signalling in synovial fibroblasts of RA patients and adjuvant-induced arthritis (AIA) in rats. Experimental approach We used computational docking, Ca2+ dynamics and functional assays to study the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase pump (SERCA). In rheumatoid arthritis synovial fibroblasts (RASFs)/rheumatoid arthritis fibroblast-like synoviocytes (RAFLS), mechanisms of Ca2+ -mediated autophagy were analysed by histological, immunohis…

2018

ABSTRACTAlthough music is the fundamental element of music therapy, music analysis methods are a particularly under-researched area. This study investigates how and when psychodynamically orientated music therapists employ the analysis of musical material in both clinical work and research. Constructivist grounded theory was employed in the collection and analysis of the data. Eight participants, all highly experienced in psychodynamic music therapy, were recruited using referral sampling. In-depth interviews focused on therapists’ experiences of working with different client groups, and the applicability of different assessment methods. Strauss and Corbin’s coding paradigm was used to dete…

Effects of maternal singing during kangaroo care on maternal anxiety, wellbeing, and mother-infant relationship after preterm birth: a mixed methods …

2020

Introduction: Preterm birth may disturb the typical development of the mother– infant relationship, when physical separation and emotional distress in the neonatal intensive care unit may increase maternal anxiety and create challenges for early interaction. This cluster-randomized controlled trial examined the effects of maternal singing during kangaroo care on mothers’ anxiety, wellbeing, and the early mother– infant relationship after preterm birth. Method: In the singing intervention group, a certified music therapist guided the mothers (n = 24) to sing or hum during daily kangaroo care during 33–40 gestational weeks (GW). In the control group, the mothers (n = 12) conducted daily kanga…

Development and validation of the Healthy-Unhealthy Music Scale.

2015

Background Music is an integral part of life in youth, and although it has been acknowledged that musical behavior reflects broader psychosocial aspects of adolescent behavior, no measurement instruments have been specifically designed for assessing musical engagement as an indicator of adolescent wellbeing and/or symptomatology. This study was conducted in order to develop and validate a scale for assessing musical engagement as an indicator of proneness for depression in youth. Method Items were developed based on the literature and a prior grounded theory analysis and three surveys (N = 54, N = 187, N = 211) were conducted to select, refine, test, and validate the items. Scale structure …

L’évaluation psychologique en situation transculturelle : une révision de la littérature

2022

Resume Les divers mouvements de deplacement humains nous poussent un peu plus chaque jour a nous rencontrer et a relever les defis que nous proposent les echanges avec l’Autre culturellement different. Dans le champ de la psychologie, l’evaluation psychologique fait partie de l’une des interrogations princeps puisque centrale de nos prises en charges quotidiennes. Considerant que la plupart des tests et manuels diagnostiques ont ete developpes dans le contexte occidental, l’application de ces outils a d’autres populations reste controversee. Cette revision de la litterature a comme but de decrire l’etat des connaissances actuelles sur l’examen psychologique en situation transculturelle. Plu…

Les effets de techniques d’engagement et de dissonance cognitive sur l’attitude, la force de l’attitude et les raisonnements

2020

Resume Cette etude s’interesse a l’impact des techniques d’engagement et de dissonance sur l’attitude, la force de l’attitude et les raisonnements associes au changement d’attitude. Les participants etaient places dans une situation experimentale basee sur l’engagement ou la dissonance ou dans une condition controle, le changement portant sur le rapport a la nourriture a base d’insectes. L’attitude (instrumentale, affective, globale) et la force de l’attitude (ambivalence, importance, extremite, certitude, intensite) ont ete mesurees. Les discours recueillis a partir des consignes experimentales etaient analyses avec TROPES et la methode des schemes cognitifs de base. Les resultats revelent…