Search results for " EXTENSION"
showing 10 items of 272 documents
Genotyping of 9 STR systems in combination with 11 diallelic polymorphisms on the Y-chromosome by fragment analysis and minisequencing
2003
1. IntroductionThe study of Y chromosomal haplotypes and their relationship to human evolution andvariation is increasing rapidly in the fields of anthropology and forensic genetics.Although autosomal STRs are commonly used and very informative for paternity testingand forensic identification, the use of the nonrecombining portion of the Y chromosome isimportant and provides additional data in cases when the offspring is a male or for mixedmale/female crime stains. For this purpose, in the past 2 years, more and more attentionhas been paid to the examination of diallelic polymorphisms (SNPs) on the Y chromosome[1].Here, we describe an approach for the typing of 10 Y-chromosomal diallelic po…
Analysis of 29 Y-chromosome SNPs in a single multiplex useful to predict the geographic origin of male lineages
2006
Abstract The European Consortium “High throughput analysis of single nucleotide polymorphisms for the forensic identification of persons—SNPforID” has performed a selection of candidate Y-chromosome SNPs (single nucleotide polymorphisms) for making inferences on the geographic origin of an unknown sample. A “Major Y chromosome haplogroup typing kit” has been developed, which allows the multiplex amplification of 29 SNPs in a single reaction followed by a single base extension (SBE) reaction (minisequencing) and separation of the resulting extension products by capillary electrophoresis.
MLOG: a strongly typed confluent functional language with logical variables
1994
Poirriez, V., MLOG: a strongly typed confluent functional language with logical variables, Theoretical Computer Science 122 (1994) 201-223. A new programming language called MLOG is introduced. MLOG is a conservative extension of ML with logical variables. To validate our concepts, a compiler named CAML Light FLU0 was implemented. Numerous examples are presented to illustrate the possibilities of MLOG. The pattern matching of ML is kept for X-calculus bindings and an unification primitive is introduced for the logical variables bindings. A suspension mechanism allows cohabitation of pattern-matching and logical variables, Although the evaluation strategy for the application is fixed, the or…
Rapid construction of algebraic axioms from samples
1991
Abstract An axiom is called reliable if it is confirmed in several places in a given sample of algebra. A very effective algorithm for enumerating such axioms is described.
Un nouvel invariant des algèbres de Lie et des super-algèbres de Lie quadratiques
2011
In this thesis, we defind a new invariant of quadratic Lie algebras and quadratic Lie superalgebras and give a complete study and classification of singular quadratic Lie algebras and singular quadratic Lie superalgebras, i.e. those for which the invariant does not vanish. The classification is related to adjoint orbits of Lie algebras o(m) and sp(2n). Also, we give an isomorphic characterization of 2-step nilpotent quadratic Lie algebras and quasi-singular quadratic Lie superalgebras for the purpose of completeness. We study pseudo-Euclidean Jordan algebras obtained as double extensions of a quadratic vector space by a one-dimensional algebra and 2-step nilpotent pseudo-Euclidean Jordan al…
Further characterization of the histidine gene cluster of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis of hisD.
1992
We have further characterized the genomic region of Streptomyces coelicolor A3(2) that contains genes involved in the biosynthesis of histidine. A 2,357-base pair fragment contained in plasmid pSCH3328 that complemented hisD mutations has been sequenced. Computer analysis revealed an open reading frame that encodes a protein with significant homology to the Escherichia coli, Salmonella typhimurium and Mycobacterium smegmatis hisD product, Saccharomyces cerevisiae HIS4C, and Neurospora crassa his3 gene products. Two other contiguous open reading frames oriented divergently with respect to hisD did not show significant similarity with any of the his genes or to other sequences included in the…
Forensic validation of the SNPforID 52-plex assay.
2007
The advantages of single nucleotide polymorphism (SNP) typing in forensic genetics are well known and include a wider choice of high-throughput typing platforms, lower mutation rates, and improved analysis of degraded samples. However, if SNPs are to become a realistic supplement to current short tandem repeat (STR) typing methods, they must be shown to successfully and reliably analyse the challenging samples commonly encountered in casework situations. The European SNPforID consortium, supported by the EU GROWTH programme, has developed a multiplex of 52 SNPs for forensic analysis, with the amplification of all 52 loci in a single reaction followed by two single base extension (SBE) react…
Forensic typing of autosomal SNPs with a 29 SNP-multiplex--results of a collaborative EDNAP exercise.
2008
We report the results of an inter-laboratory exercise on typing of autosomal single nucleotide polymorphisms (SNP) for forensic genetic investigations in crime cases. The European DNA Profiling Group (EDNAP), a working group under the International Society for Forensic Genetics (ISFG), organised the exercise. A total of 11 European and one US forensic genetic laboratories tested a subset of a 52 SNP-multiplex PCR kit developed by the SNPforID consortium. The 52 SNP-multiplex kit amplifies 52 DNA fragments with 52 autosomal SNP loci in one multiplex PCR. The 52 SNPs are detected in two separate single base extension (SBE) multiplex reactions with 29 and 23 SNPs, respectively, using SNaPshot …
Planar Cell Polarity Signaling in Collective Cell Movements During Morphogenesis and Disease
2012
Collective and directed cell movements are crucial for diverse developmental processes in the animal kingdom, but they are also involved in wound repair and disease. During these processes groups of cells are oriented within the tissue plane, which is referred to as planar cell polarity (PCP). This requires a tight regulation that is in part conducted by the PCP pathway. Although this pathway was initially characterized in flies, subsequent studies in vertebrates revealed a set of conserved core factors but also effector molecules and signal modulators, which build the fundamental PCP machinery. The PCP pathway in Drosophila regulates several developmental processes involving collective cel…
A new method for the mapping of 5' ends of RNAs.
2008
In this article, we describe a new procedure to map 5' ends of RNAs. The procedure consists in the use of specific RNase H digestion of a hybrid formed by the RNA and a complementary DNA oligonucleotide. Northern blot hybridization of the resulting RNA fragment allows an accurate measurement of its length. Although we generally use this procedure as a control of previously performed primer extension analyses, the absence of nonspecific bands, which often occur in primer extensions on RNA templates with extended secondary structures, suggests that our method may be preferable when these difficult templates are analyzed.