Search results for " HOMO"
showing 10 items of 1143 documents
Effects of cold acclimation and dsRNA injections on Gs1l gene splicing in Drosophila montana
2017
Abstract Alternative splicing, in which one gene produce multiple transcripts, may influence how adaptive genes respond to specific environments. A newly produced transcriptome of Drosophila montana shows the Gs1-like (Gs1l) gene to express multiple splice variants and to be down regulated in cold acclimated flies with increased cold tolerance. Gs1l’s effect on cold tolerance was further tested by injecting cold acclimated and non-acclimated flies from two distantly located northern and southern fly populations with double stranded RNA (dsRNA) targeting Gs1l. While both populations had similar cold acclimation responses, dsRNA injections only effected the northern population. The nature of …
The FE-Meshless multiscale approach applied to masonry structures
2015
Heterogeneous structures have an overall response that is strongly dependent on the inelastic events developing at the local level. In these structures, the most relevant kinematical and mechanical phenomena take place at a scale which is small if compared to the dimensions of the entire structure. In literature, a mesoscopic and a macroscopic scales of interest are distinguished, directly linked to as many theoretical approaches. The mesoscopic approach [1] considers materials and their interfaces individually, but many difficulties arise in the mesh creation and a fine discretization of the structure is needed, which leads to prohibitive computational costs. The macroscopic approach consi…
Phosphorylation of mismatch repair proteins MSH2 and MSH6 affecting MutSα mismatch-binding activity
2002
Mismatch repair (MMR) is involved in the removal of mispaired bases from DNA and thus plays an important role in the maintenance of genomic stability and the prevention of mutations and cancer. Moreover, MMR triggers genotoxicity and apoptosis upon processing of DNA lesions such as O6-methylguanine. Whereas the enzymology of MMR has been elucidated in great detail, only limited data are available concerning its regulation. Here we show that the major mismatch-binding proteins MSH2 and MSH6, forming the MutSalpha complex, are phosphorylated in vitro by protein kinase C and casein kinase II, but not by protein kinase A. Phosphorylation of MSH2 and MSH6 was also found within the cell, with MSH…
Expression of hMLH1 and hMSH2 proteins in ameloblastomas and tooth germs
2017
Background Mismatch repair proteins (MMRPs) are a group of nuclear enzymes that participate in the repair of base mismatches that occur during DNA replication in all proliferating cells. The most studied MMRPs are hMSH2 and hMLH1, which are known to be highly expressed in normal tissues. A loss of MMRPs leads to the accumulation of DNA replication errors in proliferating cells. Ki-67 is a biomarker regarded to be the gold-standard tool for determining cell proliferation by immunohistochemical methods. The aim of this study was to investigate the immunohistochemical expression of hMLH1, hMSH2 and Ki-67 proteins in ameloblastomas and tooth germs, to contribute to the understanding of the deve…
Methionine in a protein hydrophobic core drives tight interactions required for assembly of spider silk
2019
Web spiders connect silk proteins, so-called spidroins, into fibers of extraordinary toughness. The spidroin N-terminal domain (NTD) plays a pivotal role in this process: it polymerizes spidroins through a complex mechanism of dimerization. Here we analyze sequences of spidroin NTDs and find an unusually high content of the amino acid methionine. We simultaneously mutate all methionines present in the hydrophobic core of a spidroin NTD from a nursery web spider’s dragline silk to leucine. The mutated NTD is strongly stabilized and folds at the theoretical speed limit. The structure of the mutant is preserved, yet its ability to dimerize is substantially impaired. We find that side chains of…
Gestión del cuerpo y planificación urbana: Desplazamiento de significado en la reivención de cuepos y ciudades
2020
Contemporary cities have administrations increasingly marked by urban planning guidelines that are guided by business and marketing and, to attract investments and tourists, implement strategies that aim to homogenize, spectacularize and control urban space. However, other experiences in the city potentially build new meaning to it, which would escape those strategically planned, creating alternative possibilities of uses, diverging from the way thar are predicted and proposed by the governmental power in their urban marketing strategies. The article discusses how the occupation of urban spaces by transvestites for prostitution may imply reflections on the role of norms of gender in the (re…
The dynamics of personal norms and the determinants of cultural homogeneity
2017
This article studies the emergence of cultural homogeneity in personal norms when the behavior of heterogeneous individuals is driven both by economic incentives and by personal norms. Agents participate in a team production game by choosing their level of costly effort. Norms evolve along the life cycle of the individuals according to two psychological forces: cognitive dissonance or consistency and informational conformity. The model sheds light on how primitive economic and behavioral parameters such as the distribution of skills, the income distribution, and the levels of materialism, conformism, and consistency in the group determine the long-run culture and its degree of cultural homo…
Real-time observation of the charge transfer to solvent dynamics
2013
Intermolecular electron-transfer reactions have a crucial role in biology, solution chemistry and electrochemistry. The first step of such reactions is the expulsion of the electron to the solvent, whose mechanism is determined by the structure and dynamical response of the latter. Here we visualize the electron transfer to water using ultrafast fluorescence spectroscopy with polychromatic detection from the ultraviolet to the visible region, upon photo-excitation of the so-called charge transfer to solvent states of aqueous iodide. The initial emission is short lived (similar to 60 fs) and it relaxes to a broad distribution of lower-energy charge transfer to solvent states upon rearrangeme…
The endopolygalacturonase 1 from botrytis cinerea activates grapevine defense reactions unrelated to its enzyumatic activity
2003
A purified glycoprotein from Botrytis cinerea(strain T4), identified as endopolygalacturonase 1 (T4BcPG1) by mass spectrometry analysis, has been shown to activate defense reactions in grapevine (Vitis vinifera cv. Gamay). These reactions include calcium influx, production of active oxygen species, activation of two mitogen-activated protein kinases, defense gene transcript accumulation, and phytoalexin production. Most of these defense reactions were also activated in grapevine in response to purified oligogalacturonides (OGA) with a degree of polymerization of 9 to 20. In vivo, these active OGA might be a part of the released products resulting from endopolygalacturonase activity on plan…
The Ellipsoid in Orthogonal Axonometric: Homology Application
2012
You've long heard about ellipsoid, both from a mathematical analysis that under the geometric representative profile. However, so far no one has ever affronted the problem from the point of the application of descriptive geometry with homology. The use of homology, in fact, can make it extremely simplified and actual use of geometric tools giving the user a graphical mastery of the outcome that would otherwise be dismissed even with the use of innovative technologies of representation. Through the analysis of the proposed methodology, you can use to identify the strengths, the corresponding approvals now required between reality and projection. An ellipsoid is a closed type of quadric surfa…