Search results for " ImmunoFluorescence"

showing 8 items of 18 documents

Value of insoluble PABPN1 accumulation in the diagnosis of oculopharyngeal muscular dystrophy.

2019

Background and purpose The aim was to assess the value of insoluble PABPN1 muscle fibre nuclei accumulation in the diagnosis of atypical cases of oculopharyngeal muscular dystrophy (OPMD). Methods Muscle biopsies from a selected cohort of 423 adult patients from several Italian neuromuscular centres were analysed by immunofluorescence: 30 muscle biopsies of genetically proven OPMD, 30 biopsies from patients not affected by neuromuscular disorders, 220 from genetically undiagnosed patients presenting ptosis or swallowing disturbances, progressive lower proximal weakness and/or isolated rimmed vacuoles at muscle biopsy and 143 muscle biopsies of patients affected by other neuromuscular diseas…

Pathologymedicine.medical_specialtyFluorescent Antibody TechniquePoly(A)-Binding Protein IOculopharyngeal muscular dystrophy03 medical and health sciences0302 clinical medicinePtosisMuscular Dystrophy OculopharyngealPABPN1 accumulations; PABPN1 immunofluorescence; oculopharyngeal musclular dystrophy; rimmed vacuoles; tubulofilamentous intranuclear inclusionsmedicineHumans030212 general & internal medicineMuscle fibreMuscle SkeletalCell NucleusMuscle biopsymedicine.diagnostic_testbusiness.industryExternal ophthalmoplegiaRimmed vacuolesmedicine.diseaseNeurologyCohortNeurology (clinical)Inclusion body myositismedicine.symptombusiness030217 neurology & neurosurgeryEuropean journal of neurologyReferences
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In vivo optical coherence tomography imaging in a case of mucous membrane pemphigoid and a negative Nikolsky's sign.

2017

There is currently a growing interest in new diagnostic tools of the oral cavity and mucosa which are non-invasive, repeatable and reliable. A diagnosis of a suspected, autoimmune pathology was made regarding a 57-year-old patient with desquamative gingivitis. However, a negative Nikolsky's sign did not seem to indicate a diagnosis of mucous membrane pemphigoid neither was there any indication as to the optimum location for an incisional biopsy. As an imaging method, the use of optical coherence tomography (OCT) has enabled the obtaining of tomographic (cross-sectional) scans of tissue. Such images are acquired prior to and after verifying Nikolsky's sign, thereby enabling the clinician to …

Pathologymedicine.medical_specialtyIncisional biopsyDystoninAdministration TopicalPemphigoid Benign Mucous MembraneDermatologyDiagnostic toolsEpithelium030207 dermatology & venereal diseases03 medical and health sciences0302 clinical medicineOptical coherence tomographyIn vivomedicineHumansFluorescent Antibody Technique IndirectGlucocorticoidsAutoantibodiesClobetasolIndirect immunofluorescencemedicine.diagnostic_testbusiness.industryMouth Mucosa030206 dentistryGeneral MedicineMiddle Agedmedicine.diseaseGingivitisDesquamative gingivitisoptical coherence tomography mucous membrane pemphigoid Nikolsky's signNikolsky's signMucous membrane pemphigoidFemalemedicine.symptombusinessTomography Optical CoherenceThe Journal of dermatology
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Computer-Assisted Classification Patterns in Autoimmune Diagnostics: The AIDA Project.

2016

International audience; Antinuclear antibodies (ANAs) are significant biomarkers in the diagnosis of autoimmune diseases in humans, done by mean of Indirect ImmunoFluorescence (IIF) method, and performed by analyzing patterns and fluorescence intensity. This paper introduces the AIDA Project (autoimmunity: diagnosis assisted by computer) developed in the framework of an Italy-Tunisia cross-border cooperation and its preliminary results. A database of interpreted IIF images is being collected through the exchange of images and double reporting and a Gold Standard database, containing around 1000 double reported images, has been settled. The Gold Standard database is used for optimization of …

Pathologymedicine.medical_specialtyTunisiaArticle SubjectAnti-nuclear antibody[SDV]Life Sciences [q-bio]lcsh:MedicineCAD02 engineering and technologyGeneral Biochemistry Genetics and Molecular Biology030218 nuclear medicine & medical imagingAutoimmune Diseases03 medical and health sciences0302 clinical medicine0202 electrical engineering electronic engineering information engineeringImage Processing Computer-AssistedMedicineHumansFluorescent Antibody Technique IndirectIndirect immunofluorescenceGeneral Immunology and Microbiologybusiness.industrylcsh:RIIfPattern recognitionGeneral MedicineGold standard (test)Computer aided detectionSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)3. Good healthFluorescence intensityItalyComputer-aided diagnosisAntibodies Antinuclear020201 artificial intelligence & image processingArtificial intelligencebusinessComputer Aided Diagnosis Immunofluorescence Pattern Classification IIF images Autoimmune diseases SVM ANN HEp-2Research ArticleBioMed research international
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Metilazione del DNA in artrite reumatoide

2005

Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…

Settore BIO/18 - Geneticainstead chromosomes of controls were almost uniformly decorated by brilliant grains. Studies on methylation of PTHrP gene promoter 2 performed on five CpG island internal sites using the Methylation-Sensitive Restriction Endonuclease Multiplex (MSREM)-PCR showed that one of the sites nearest the trascription starting point is heavy methylated in a significantly high number of RA patients. Thus RA seems to be characterized by genomewide hypomethylation associated with local hypermethylation like the most part of tumors. This result raises the possibility that susceptibility to lymphomas is related to abnormal DNA methylation levels and suggests the opportunity to evaluate the DNA methylation status in RA patientin fact the demethylating therapies together with diet and life style can act towards an increase of tumor risk. Future studies using a larger number of subjects could confirm these findings.Rheumatoid Arthritis (RA) is a chronic multisystem inflammatory disease characterized by high recurrence of lymphomas as well as hypercalcemia due to PTHrP overexpression. Because of DNA methylation plays a critical role in development of neoplasias we determined in RA patients the global DNA methylation status and local methylation pattern of the CpG island of one of the three promoters of PTHrP gene utilizing molecular and cytogenetic techniques. Investigations performed on DNA from peripheral blood of patients and donors amplified by Methylation-Sensitive Arbitrarily Primed (MeS-AP)-PCR indicated that RA is strongly associated with global DNA hypomethylation. Similarly chromosomal DNA methylation pattern analysis by indirect immunofluorescence technique with anti 5-methylcitosine antibody showed all peripheral lymphocyte metaphases from RA patients with chromosomes weakly fluorescent without discrete grain
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Comparative Study of Human and Automated Screening for Antinuclear Antibodies by Immunofluorescence on HEp-2 Cells

2015

Background : Several automated systems had been developed in order to reduce inter-observer variability in indirect immunofluorescence (IIF) interpretation. We aimed to evaluate the performance of a processing system in antinuclear antibodies (ANA) screening on HEp-2 cells. Patients and Methods : This study included 64 ANA-positive sera and 107 ANA-negative sera that underwent IIF on two commercial kits of HEp-2 cells (BioSystems® and Euroimmun®). IIF results were compared with a novel automated interpretation system, the “ Cyclopus CADImmuno®” (CAD). Results : All ANA-positive sera images were recognized as positive by CAD (sensitivity = 100%), while 17 (15.9%) of the ANA-negative sera ima…

Statistics and ProbabilityPathologymedicine.medical_specialtyIndirect immunofluorescenceAnti-nuclear antibodymedicine.diagnostic_testbusiness.industryConcordanceHealth InformaticsIIfImmunofluorescenceHealth Professions (miscellaneous)Settore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Continuous integrationPattern identificationAutoantibodies HEp-2 Immunofluorescence autoimmune diseases automated screening standardization.Health Information ManagementMedicineFine speckledbusinessInternational Journal of Statistics in Medical Research
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Apoptotic-like Leishmania exploit the host´s autophagy machinery to reduce T-cell-mediated parasite elimination

2015

Apoptosis is a well-defined cellular process in which a cell dies, characterized by cell shrinkage and DNA fragmentation. In parasites like Leishmania, the process of apoptosis-like cell death has been described. Moreover upon infection, the apoptotic-like population is essential for disease development, in part by silencing host phagocytes. Nevertheless, the exact mechanism of how apoptosis in unicellular organisms may support infectivity remains unclear. Therefore we investigated the fate of apoptotic-like Leishmania parasites in human host macrophages. Our data showed--in contrast to viable parasites--that apoptotic-like parasites enter an LC3(+), autophagy-like compartment. The compartm…

log.ph logarithmic phaseT-LymphocytesApoptosisMACS magnetic-associated cell sortingMacrophageMFI mean fluorescence intensityLeishmaniasisMOI multiplicity of infectionanti-inflammatoryLeishmaniaeducation.field_of_studyPhagocytesCFSE carboxyfluorescein succinimidyl esterTGFB transforming growth factorAcquired immune systemapoptotic-like LeishmaniaPS phosphatidylserinehuman primary macrophagesCell biologyβ; TT tetanus toxoidCorrigendumProgrammed cell deathautophagyPopulationAntigen presentationANXA5 annexin VBasic Science Research PapersBiologyPhagocytosisCM complete mediumMAP1LC3/LC3 microtubule-associated protein 1 light chain 3AnimalsHumansMHC major histocompatibility complexIF immunofluorescenceeducationMolecular Biologyimmune evasionPBMCs peripheral blood mononuclear cellsT-cell proliferationIntracellular parasiteMacrophagesstat.ph stationary phaseAutophagyLm LeishmaniaCell BiologyLeishmaniabiology.organism_classificationIL interleukinLAP LC3-associated phagocytosisLAPhMDM human monocyte derived macrophageAutophagy
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Automated approach for indirect immunofluorescence images classification based on unsupervised clustering method

2018

Autoimmune diseases (ADs) are a collection of many complex disorders of unknown aetiology resulting in immune responses to self-antigens and are thought to result from interactions between genetic and environmental factors. ADs collectively are amongst the most prevalent diseases in the U.S., affecting at least 7% of the population. The diagnosis of ADs is very complex, the standard screening methods provides seeking and recognizing of Antinuclear Antibodies (ANA) by Indirect ImmunoFluorescence (IIF) based on HEp-2 cells. In this paper an automatic system able to identify and classify the Centromere pattern is presented. The method is based on the grouping of centromeres present on the cell…

medical disorderComputer sciencePopulationFeature extraction02 engineering and technologybiomedical optical imagingmedical image processing030218 nuclear medicine & medical imaging03 medical and health sciences0302 clinical medicineImage textureblood0202 electrical engineering electronic engineering information engineeringSegmentationimage texturecellular biophysicsCluster analysiseducationimage segmentationdiseaseeducation.field_of_studyIndirect immunofluorescenceContextual image classificationbusiness.industryfeature extractionPattern recognitionImage segmentationSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)020201 artificial intelligence & image processingfluorescenceComputer Vision and Pattern RecognitionArtificial intelligencebusinessSoftwareimage classificationIET Computer Vision
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Analysis of the impact of sex and age on the variation in the prevalence of antinuclear autoantibodies in Polish population: a nationwide observation…

2022

AbstractThe detection of antinuclear autoantibody (ANA) is dependent on many factors and varies between the populations. The aim of the study was first to assess the prevalence of ANA in the Polish adult population depending on age, sex and the cutoff threshold used for the results obtained. Second, we estimated the occurrence of individual types of ANA-staining patterns. We tested 1731 patient samples using commercially available IIFA using two cutoff thresholds of 1:100 and 1:160. We found ANA in 260 participants (15.0%), but the percentage of positive results strongly depended on the cutoff level. For a cutoff threshold 1:100, the positive population was 19.5% and for the 1:160 cutoff th…

musculoskeletal diseasesAdultMaleRMAnti-nuclear antibodyCross-sectional studyIndirect immunofluorescence assayImmunologyPopulationAdult populationPolish populationObservational ResearchAutoimmune DiseasesAgeSex FactorsRheumatologyCutof thresholdPrevalenceImmunology and AllergyCutoffMedicineHumanseducationskin and connective tissue diseasesQH426education.field_of_studybusiness.industryAutoantibodyAge FactorsMiddle AgedQRCutoff thresholdstomatognathic diseasesAntinuclear antibodiesCross-Sectional StudiesIndirect immunofuorescence assayAntibodies AntinuclearQR180Observational studySexFemalePolandbusinessDemographyRheumatology International
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