Search results for " LAB"

LC of high to moderately polar basic drugs in urine with water and detergent, and direct injection

2016

Background: Micellar LC was first proposed as a ‘green’ mode using mobile phases of water and surfactant. However, in most procedures a small amount of organic solvent is required to decrease the retention to convenient values. Results & methodology: Mixed micellar mobile phases prepared with both cationic (sodium dodecyl sulphate) and nonionic surfactant (Brij-35) modulate the retention of high to moderately polar basic drugs to practical times, eliminating the need of organic solvent. While the mobile phase is continuously recycled through the system, the stationary phase performance is maintained after repetitive injection of the samples. Discussion & conclusion: Through an exte…

Design Procedures for Soil-Lime Stabilization for Road and Railway Embankments. Part 2-Experimental Validation

2012

AbstractAs detailed in Part 1, the most common Italian Technical Specifications refer to different design methodologies for design of lime-soil mixtures, some being quite different from those internationally adopted. Therefore, it seemed appropriate to the Authors to compare these methodologies, via a wide experimental program.It is shown that the Italian methodology of the National Road Agency is different not only from those used in Italy, but also from the main international standards. The experimental results highlight that a revision of that methodology is needed in order to bring it into line with what is prescribed by the European standard.

Beeswax cleaning by solvent extraction of pesticides

2018

We set out to test if the methodology used to clean sheep wool wax (Lanolin) from pesticides could be used to clean beeswax as well. We first made an aggregate sample of brood comb wax from three different US beekeepers. Sub-samples of these aggregate wax samples were analyzed for pesticide contamination. The remaining wax, was then dissolved into hexane solution and run through four N, N-Dimethylformamide (DMF) washes. During these extractions, the pesticides partitioned into the DMF, and so were removed from the beeswax. Following the solvent extractions, the beeswax was tested again for pesticides. An average of 95% of the pesticide contamination was removed by the chemical wash procedur…

A phase IB, multicenter, open-label study to assess the safety, tolerability, and efficacy of the pleiotropic pathway modifier CC122 administered ora…

2017

379 Background: CC122 is a novel cereblon-modulating agent with multiple biologic activities including potent immunomodulatory and antiangiogenic effects. CC-122 binding to cereblon promotes ubiquitination and subsequent degradation of lymphoid transcription factors Ikaros and Aiolos resulting in activation of T cells. Methods: Following establishment of oral CC122 3 mg daily (QD) as the MTD in phase 1a (Blood 122:2905 2013), an expansion cohort of advanced Hepatocellular Carcinoma (HCC) subjects was enrolled. All subjects had progressed on or were intolerant to sorafenib. Efficacy was assessed per RECIST 1.1 criteria. Results: As of Jan. 13, 2016, 25 advanced HCC subjects were enrolled. T…

FINDUS: An Open-Source 3D Printable Liquid-Handling Workstation for Laboratory Automation in Life Sciences

2020

3D-printed laboratory devices can enable ambitious research purposes even at a low-budget level. To follow this trend, here we describe the construction, calibration, and usage of the FINDUS (Fully Integrable Noncommercial Dispensing Utility System). We report the successful 3D printing and assembly of a liquid-handling workstation for less than $400. Using this setup, we achieve reliable and flexible liquid-dispensing automation with relative pipetting errors of less than 0.3%. We show our system is well suited for several showcase applications from both the biology and chemistry fields. In support of the open-source spirit, we make all 3D models, assembly instructions, and source code ava…

Innovazioni didattiche e ricadute sugli apprendimenti

2016

L’obiettivo della presente ricerca era quello di valutare la ricaduta che l’innovazione in aula, derivante dalla sperimentazione dei modelli didattici innovativi (Spaced Learning, Contenuti Didattici Digitali, Coding, Aule Laboratori disciplinari), ha avuto sui processi di apprendimento declinati in conoscenza, comprensione, contestualizzazione e valutazione. Gli strumenti di raccolta dati sono stati prove di verifica dell’apprendimento costruite dai docenti, facendo riferimento a una Griglia di indicatori di apprendimento e un questionario di valutazione della consapevolezza metacognitiva degli studenti – Awareness Learning Metacognitive ALM2014 (La Marca, 2014).

Towards a Deep Integration of Socio-Economic Action and Spatial Planning

2012

It is now time to recognize that self-organizing socio-economic actors can and must act directly on the pursuit of territorial development and cohesion or, in other words, that the broadest possible number of socio-economic actors must be involved, to this end, in co-creating products, services and innovative content. ICTs can greatly help to go forward in this direction, allowing the merging of regulation, implementation of policies and spontaneous transformation, thanks to their interactivity and their potential of diffusion of “usable knowledge” (Lindblom and Cohen, 1979). The proposed approach relies on the notion of Living Labs (LLs), or better on that of “Territorial Living Labs” (TLL…

Sexually transmitted COVID‐19

2020

Infrared Difference Spectroscopy of Proteins: From Bands to Bonds

2020

Infrared difference spectroscopy probes vibrational changes of proteins upon their perturbation. Compared with other spectroscopic methods, it stands out by its sensitivity to the protonation state, H-bonding, and the conformation of different groups in proteins, including the peptide backbone, amino acid side chains, internal water molecules, or cofactors. In particular, the detection of protonation and H-bonding changes in a time-resolved manner, not easily obtained by other techniques, is one of the most successful applications of IR difference spectroscopy. The present review deals with the use of perturbations designed to specifically change the protein between two (or more) functional…

Axial growth of hexactinellid spicules: Formation of cone-like structural units in the giant basal spicules of the hexactinellid Monorhaphis

2008

The glass sponge Monorhaphis chuni (Porifera: Hexactinellida) forms the largest bio-silica structures on Earth; their giant basal spicules reach sizes of up to 3 m and diameters of 8.5 mm. Previously, it had been shown that the thickness growth proceeds by appositional layering of individual lamellae; however, the mechanism for the longitudinal growth remained unstudied. Now we show, that the surface of the spicules have towards the tip serrated relief structures that are consistent in size and form with the protrusions on the surface of the spicules. These protrusions fit into the collagen net that surrounds the spicules. The widths of the individual lamellae do not show a pronounced size …