Search results for " LAB"
showing 10 items of 2393 documents
Imaging of VSOP labeled stem cells in agarose phantoms with susceptibility weighted and T2* weighted MR Imaging at 3T: determination of the detection…
2013
Objectives This study aimed to evaluate the detectability of stem cells labeled with very small iron oxide particles (VSOP) at 3T with susceptibility weighted (SWI) and T2* weighted imaging as a methodological basis for subsequent examinations in a large animal stroke model (sheep). Materials and Methods We examined ovine mesenchymal stem cells labeled with VSOP in agarose layer phantoms. The experiments were performed in 2 different groups, with quantities of 0–100,000 labeled cells per layer. 15 different SWI- and T2*-weighted sequences and 3 RF coils were used. All measurements were carried out on a clinical 3T MRI. Images of Group A were analyzed by four radiologists blinded for the num…
Metalloprotease meprin beta generates nontoxic N-terminal amyloid precursor protein fragments in vivo.
2011
Identification of physiologically relevant substrates is still the most challenging part in protease research for understanding the biological activity of these enzymes. The zinc-dependent metalloprotease meprin β is known to be expressed in many tissues with functions in health and disease. Here, we demonstrate unique interactions between meprin β and the amyloid precursor protein (APP). Although APP is intensively studied as a ubiquitously expressed cell surface protein, which is involved in Alzheimer disease, its precise physiological role and relevance remain elusive. Based on a novel proteomics technique termed terminal amine isotopic labeling of substrates (TAILS), APP was identified …
Synthesis of biotin-labelled dexamethasone derivatives. Novel hormone-affinity probes.
1983
A new, general methodology for 'sandwich' affinity chromatography of steroid hormone receptors is proposed, the part purification of the human spleen tumor glucocorticoid receptor is quoted as an illustration. 9-Fluoro-16 alpha-methyl-11 beta, 17-dihydroxy-1,4-androstadiene-3-one-17 beta-carboxylic acid was coupled to biotin using pentamethylenediamine (BioDex 1) as a spacer. The bifunctional derivative binds to glucocorticoid receptors and avidin-Sepharose and efficiently protects the glucocorticoid receptor against inactivation when previously added during homogenisation. We have standardized the capacity and optimum conditions for elution of receptor-BioDex-1 complexes which are bound to…
Novel Combination of Sorafenib and Celecoxib Provides Synergistic Anti-Proliferative and Pro-Apoptotic Effects in Human Liver Cancer Cells
2013
Molecular targeted therapy has shown promise as a treatment for advanced hepatocellular carcinoma (HCC). Sorafenib, a multikinase inhibitor, recently received FDA approval for the treatment of advanced HCC. However, although sorafenib is well tolerated, concern for its safety has been expressed. Celecoxib (Celebrex®) is a selective cyclooxygenase-2 (COX-2) inhibitor which exhibits antitumor effects in human HCC cells. The present study examined the interaction between celecoxib and sorafenib in two human liver tumor cell lines HepG2 and Huh7. Our data showed that each inhibitor alone reduced cell growth and the combination of celecoxib with sorafenib synergistically inhibited cell growth an…
Immune Modulating Effects of NKT Cells in a Physiologically Low Dose Leishmania major Infection Model after αGalCer Analog PBS57 Stimulation
2014
Leishmaniasis is a parasitic infection affecting ∼12 million people worldwide, mostly in developing countries. Treatment options are limited and no effective vaccines exist to date. Natural Killer T (NKT) cells are a conserved innate-like lymphocyte population with immunomodulating effects in various settings. A number of reports state a role of NKT cells in different models of Leishmania infection. Here, we investigated the effect of NKT cells in a physiologically relevant, intradermal low dose infection model. After inoculation of 103 infectious-stage L. major, comparable numbers of skin-immigrating NKT cells in both susceptible BALB/c mice and resistant C57BL/6 mice were noted. Compared …
Spontaneous labour at term is associated with fetal monocyte activation.
1999
SUMMARYThe aetiology of both term and preterm labour remains incompletely understood. Maternal infectious diseases as well as intra-uterine infections were shown to be a well established cause of uncontrollable preterm delivery, indicating that inflammatory reactions, regulated by maternal immunecompetent cells, are implicated in labour-promoting mechanisms. To investigate the possibility that the activation of the fetal immune system may be involved in labour induction, we examined cytokine production patterns of different cord blood cell populations obtained from neonates after spontaneous onset of normal term labour and vaginal delivery (n = 25), vaginal delivery but induced term labour …
Medicīnas studentu empātijas, uztvertā stresa un subjektīvās labklājības saistības
2020
Medicīniskā izglītība ir plaši zināma kā lielus resursus pieprasošs process, kas var izraisīt dažādas psiholoģiskās problēmas, kas, savukārt, var samazināt studentu empātijas un apmierinātības ar dzīvi līmeni. Bakalaura darba mērķis bija apkopot šobrīd zināmo informāciju par empātiju, uztverto stresu, subjektīvo labklājību un šo konstruktu saistībām; noteikt, vai starp medicīnas studentu dzimumu un studiju kursu pastāv atšķirības šo trīs mainīgo mērījumu rezultātos; noskaidrot, vai starp tiem pastāv saistības; balstoties uz saistību ciešumu, atlasīt tos mainīgos lielumus, kas vislabāk izskaidro apmierinātību ar dzīvi šajā izlasē un šajā pētījumā. Pētījuma izlasi veido 216 studenti no medicī…
Les Agències de Col·locació dins del model vigent d'intermediació Laboral. Una aproximació al paper que desenvolupen a les zones rurals: el cas de la…
2014
Des de principis dels anys setanta, i de forma cíclica i fluctuant, l'atur s'ha erigit com el principal problema socioeconòmic a l'Estat espanyol. La virulència de la recessió i crisi econòmica iniciada a finals del 2007 ha obert un periode de forta destrucció d'ocupació i de nombrosos tancaments d'empreses, generant grans impactes en la vida de les persones i en el funcionament de l'economia i la societat. La magnitud d'aquesta problemàtica ha sobrepassat amb escreix la capacitat de resposta dels governs i dels serveis públics i privats d'ocupació. A més, el mercat de treball, per ell mateix, ha demostrat no ser suficientment eficaç a l'hora d'aconseguir uns majors nivells d'ocupació, i co…
Gas-phase ion chemistry of protonated melatonin
2005
The gas-phase ion chemistry of protonated melatonin has been investigated by mass spectrometric (MS) techniques involving chemical ionisation, fast atom bombardment and electrospray ionisation. Either naturally- occurring or collision-induced decomposition e-D derivatives obtained by exchange with D(2)O. The analysis of experimental results allows definite pathways for the formation of the ion at m/z 174 to be assigned and sheds some more light on the overall fragmentation pathways. Experiments on labelled derivatives evidenced H-D scrambling processes during fragmentation
Fiziskā un emocionālā labsajūta sievietēm menopauzes periodā
2022
Bakalaura darba tēma ir “Fiziskā un emocionālā labsajūta sievietēm menopauzes periodā”. Pētījuma mērķis ir izpētīt, kā fiziskās aktivitātes ietekmē emocionālo labsajūtu sievietēm menopauzes periodā. Apkopojot pētījuma rezultātus, var redzēt, ka fiziskās aktivitātes, pētījumā iesaistītām dalībniecēm, labvēlīgi ietekmē pašsajūtu, uzlabo fizisko un emocionālo stāvokli menopauzes laikā. Darbā tika izmantota kvantitatīvā pētniecības metode – anketēšana. Aptaujas anketa X veselības iestādē