Search results for " Laboratory"
showing 10 items of 622 documents
Histochemisches Enzymmuster der Mamma unter dem experimentellen Einfluß von Geschlechtshormonen
1968
In histochemischen Untersuchungen an der Mamma von Ratten soll die Beeinflussung der Enzymaktivitat durch Ostrogene und Progesteron demonstriert werden. Die Untersuchungen wurden an 40 weiblichen juvenilen und kastrierten Albinoratten durchgefuhrt, von denen die 1. Gruppe Follikelhormon (Progynon, 5 γ/d), die 2. Gruppe Corpus luteum-Hormon (Proluton, 1 mg/d) erhielten. Neben Kontrolltieren gleichen Alters wurden die Brustdrusen unbehandelter infantiler Tiere untersucht. Am 3., 5., 10. und 20. Tag nach Injektionsbeginn wurden die Tiere getotet und die Brustdrusen histologisch sowie enzymhistochemisch durch den Nachweis von alkalischer Phosphatase (Azofarbstoff und Schwermetallsimultanmethode…
Zur Frage der histochemischen Fettspezifit�t des Sudanschwarz B und zur Methodik der Auftrennung seiner FraktioneN
1964
1. Spektrophotometrische Untersuchungen verschiedener kauflicher deutscher Sudanschwarz B-Sorten zeigen gleiche Absorptionskurven mit zwei charakteristischen Maxima bei 415 und 595 nm. Die einzelnen Sudanschwarz B-Fabrikate stellen mit groser Wahrscheinlichkeit gleiche oder zumindest sehr ahnliche Farbstoffgemische dar und sind somit untereinander vergleichbar. 2. In Anlehnung anBermes undMcDonald wird ein saulenchromatographisches Verfahren angegeben, um Fraktionen des kauflichen Sudanschwarz B fur praparative Zwecke zu gewinnen. Auch konnen verschiedene Komponenten des Sudanschwarz B aufgrund verschiedener Loslichkeit isoliert werden. 3. Das Sudanschwarz B von Merck hat nicht nur ein glei…
Isolierung und Eigenschaften von Lipofuscin aus Herzgewebe des Menschen
1962
Biomarkers for diagnosing sepsis in the emergency department: A consensus document by SIBioC-Medicina di Laboratorio and the Academy of Emergency Med…
2018
This article is drafted as a consensus document involving eight members of the Italian Society of Clinical Biochemistry and Laboratory Medicine (SIBioC) and eight members of the Academy of Emergency Medicine and Care (AcEMC), to whom a questionnaire was submitted for obtaining opinions on some recommendations about the use of biomarkers for diagnosing sepsis and managing antibiotic therapy in the emergency department. These recommendations were drafted following the National Guidelines Program (PNLG). According to the cumulative consent, three "A" recommendations (strongly recommended indication) emerged, which include biomarker availability (always available on prescription), clinical use …
Therapeutic monitoring of autoantibodies Tumor Necrosis Factor α inhibitor drugs: Efficacy and benefit for patients with autoimmune diseases
2018
Tumor necrosis factor alpha (TNFα) is a proinflammatory cytokine involved in the pathogenesis of chronic inflammatory disease, such as rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Chron's disease and ulcerative colitis. TNFα inhibitors (anti-TNFα) are monoclonal antibodies drugs directed against TNFα (i.e. adalimumab, infliximab, etarnecept, golimumab and certolizumab). Their effect consists in reducing the inflammatory response of autoimmune diseases. Several randomized controlled trials and observational studies evaluated the therapeutic efficacy of these drugs and reported a clear benefit for patients affected by chronic inflammatory disease treated with anti-TNFα, …
Esami di laboratorio in Pronto Soccorso: una proposta di consenso SIBioCMedicina di Laboratorio e Academy of Emergency Medicine and Care
2017
Laboratory diagnostics in the emergency setting encompasses the identification of appropriate testing according to specific acute conditions. Since the pathway of ordering tests in the Italian Emergency Departments (EDs) is rather heterogeneous, SIBioC-Medicina di Laboratorio and the Academy of Emergency Medicine and Care designed a survey aimed to generate consensus pertaining to appropriate laboratory tests in most frequent acute conditions. A questionnaire including a panel of laboratory tests was administered to 8 representative members of each of the two societies, who were asked to provide a score between 1 and 3 for the various tests, where a score of 1 entailed "highly recommended",…
Identificazionee caratterizzazione di mutazioni in regioni regolatorie del gene malattia della fibrosi cistica
2013
Mutation epidemiology is crucial for cystic fibrosis (CF) diagnosis and counselling. ~6%-7% of alleles from CF patients do not bear mutations in the coding regions of the Cystic Fibrosis Transmembrane Regulator (CFTR) disease gene. In these patients, mutations may be present in non-coding, regulatory regions of the gene as i) intronic regions (particularly in high conserved sequences), ii) the promoter region or iii) the area at the 3’ of the gene, which is the target of microRNA regulation. We studied these regions by gene sequencing in a group of CF patients with one or both unidentified mutations after the analysis of CFTR coding regions, and in a group of CF patients with a different cl…
Acute troponin i increase in absence of obstructive coronary disease: A case of takotsubo syndrome
2017
A 66-year-old woman was admitted to the Emergency Department of Policlinico P. Giaccone, in Palermo, for non-radiating chest pain that occurred after an emotional stress. Her medical history included a positive family history for cardiovascular disease, arterial hypertension, gastro-esophageal reflux disease, and anxiety-depressive syndrome. Upon admission, the electrocardiogram showed diffuse ST-T abnormalities with an elevation of the ST segment; Troponin I was 3790 ng/L, creatine phosphokinase was 374 U/L, which became normal within 48 hours. No evidence of significant coronary artery stenosis was detected on the angiography. The echocardiogram showed apical akinesia and hyperkinesia of …
Beitrag zur Fettspezifit�t des Sudanschwarz B und Anderer Roter Sudanfarbstoffe bei Reinsubstanzen
1965
Auf Filterpapier aufgetragene Reinsubstanzen (Lipide, Eiweissubstanzen und Polysaccharide) wurden mit Sudan III, Sudan IV, Sudanrot B, Sudanrot VII B, Scharlach R med und Sudanschwarz B (SSB) gefarbt.