Search results for " MORPHOLOGY"
showing 10 items of 543 documents
Ras, Rap, and Rac Small GTP-binding Proteins Are Targets for Clostridium sordellii Lethal Toxin Glucosylation
1996
Lethal toxin (LT) from Clostridium sordellii is one of the high molecular mass clostridial cytotoxins. On cultured cells, it causes a rounding of cell bodies and a disruption of actin stress fibers. We demonstrate that LT is a glucosyltransferase that uses UDP-Glc as a cofactor to covalently modify 21-kDa proteins both in vitro and in vivo. LT glucosylates Ras, Rap, and Rac. In Ras, threonine at position 35 was identified as the target amino acid glucosylated by LT. Other related members of the Ras GTPase superfamily, including RhoA, Cdc42, and Rab6, were not modified by LT. Incubation of serum-starved Swiss 3T3 cells with LT prevents the epidermal growth factor-induced phosphorylation of m…
Fluctuation Methods To Study Protein Aggregation in Live Cells: Concanavalin A Oligomers Formation
2011
Prefibrillar oligomers of proteins are suspected to be the primary pathogenic agents in several neurodegenerative diseases. A key approach for elucidating the pathogenic mechanisms is to probe the existence of oligomers directly in living cells. In this work, we were able to monitor the process of aggregation of Concanavalin A in live cells. We used number and brightness analysis, two-color cross number and brightness analysis, and Raster image correlation spectroscopy to obtain the number of molecules, aggregation state, and diffusion coefficient as a function of time and cell location. We observed that binding of Concanavalin A to the membrane and the formation of small aggregates paralle…
Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth.
2017
Aims To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint-Maur-des-Fosses, France) MTA (Angelus, Londrina, PR, Brazil), Theracal LC (Bisco Inc., Schamburg, IL, USA) and IRM (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from human exfoliated primary teeth (SHEDs). Methodology SHEDs were cultured in the presence of the eluates of various pulpotomy materials for 24, 48 and 72 h. Cell viability was determined by mitochondrial dehydrogenase enzymatic (MTT) assay. Apoptosis and changes in cell phenotype were evaluated by flow cytometry. Also, an in vitro scratch wound-healing assay was used to determine th…
Adhesion and adaptive response of osteoblastlike cells (SAOS-2) grown on dfferent ti-6al-4v surfaces.
2004
Osteoblastic-like cells (SAOS-2) were seeded on three differently treated surfaces of Ti- 6Al-4V (T1: not treated; T2: chemically treated; T3: electrochemically treated). After 6h, the cells grown on T1 and T2 surfaces showed a typical poligonal morphology, they were strongly adherent to the surface and after 24h-48h they spread and formed a confluent monolayer. On the contrary, cells seeded on T3 showed heterogeneous morphology, decreased adhesion, loss of thin cellular expansions, and showed cellular shrinkage and apoptotic blebbing; only after 48h they were able to attach and form a monolayer after 4 days of seeding. Cell proliferation was significantly lower on T3 than T1 and T2 surface…
Adaptive response of osteoblasts grown on a titanium surface: morphology, cell proliferation and stress protein synthesis.
2005
Titanium is one of the most widely used biomaterials in orthopaedic, dental and trauma surgery. Superficial adhesion and cell proliferation represent the first steps of cell-biomaterial interactions. The efficacy of this early phase influences the subsequent ability to differentiate, and hence the knowledge of these cell activities is important for studying and improving the biocompatibility of biomaterials. The aim of this study was to test the adaptive ability of osteoblastic cells grown on titanium surfaces, including morphologic, proliferative and adaptative aspects.
Detect VS Reveal. The survey as an investigative tool in the didactic experience of the architectural project
2020
The aim of the contribution is to reflect retrospective on the experience developed within the Laboratory of Architectural Design I of the Bachelor of Architecture Design course at the School (AUIC) of the Politecnico di Milano (Campus Piacenza). The teaching structure includes, in addition to the module of Architectural and Urban Composition (8CFU) and Architectural Design (4 CFU) a further module of integrative teaching of Representation Techniques (4 CFU) of ICAR/06. The aim of the latter is to provide students with the theoretical and operational information necessary for the survey and graphic representation of the architectural work and its reference context. The Representation module…
Tomographic image processing for the morphological and metrological study of Valsalva sinuses
2012
This Phd thesis deals with the design and the use of image processing tools in order to allow a reliable and objective study of the sinuses of Valsalva which are important cavities of the aortic root. The proposed methods can be applied on cine-MR sequences and CT examinations without any change in the settings between two examinations.Firstly, we studied the morphology of this anatomical area and its constant properties in all images of the dataset. Sinuses are one of the main bright organs with limited movements. Hence a new algorithm has been designed. It detects and characterizes each bright organ by a single trajectory. Various tools of mathematical morphology are used for this step, a…
Efectos de una crecida en un cauce antropizado. La riada del Palancia de octubre de 2000
2011
[EN] In October of 2000, the Palància River underwent a major flood, which substantially modified the morphology of the river channel. These changes took place on a river bed previously modified by public works aimed at cleaning and simplifying the river section. The flood partially restored the braided morphology, reconstructing bars and channels. Moreover, river incision processes increased, particularly downstream the Palància bridges. This process reveals a sedimentary deficit caused by reservoirs; land use changes and gravel mining. The aim of this work is to describe these processes of change and to analyze the morphosedimentary consequences, through the interpretation of aerial photo…
Umwelt
2020
The term UMWELT spreads in the German language from the beginning of the nineteenth century, to indicate—both in a general relational and sociological sense, and in more specific reference to the peculiarities of PERCEPTION considered as an essential biological characteristic—the external world that surrounds and accompanies living organisms.
Muratorian urban morphology: the walled city of Ahmedabad
2015
This paper investigates the complexity of the urban fabric, from the urban scale to the building and neighbourhood scale, by using the concepts and tools of urban morphology according to the Muratorian School. Specifically, basic concepts such as ‘urban polarities’, ‘routes hierarchical system’ and ‘building neighbourhood’ are used. They are deployed in the walled city of Ahmedabad, India, which has a rich architectural heritage clearly recognizable in Indo-Islamic monuments and particularly in aggregative structures called pols.