Search results for " Models"

Re-education policy in the Comunitat Valenciana region: an analysis of educational practices in juvenile justice centers

2013

En el presente artículo se analizan aspectos que definen la política de reeducación de la Comunitat Valenciana. Se examina el papel de los Centros de Reeducación y sus prácticas educativas, para ello se toma como indicadores: los mecanismos de coordinación con el medio abierto, las relaciones con la comunidad, la proporción del gasto e inversión que la administración pública realiza sobre el sistema de justicia juvenil, el compromiso de las políticas públicas con la integración de los Adolescentes en Conflicto con la Ley, etc. La investigación es fundamentalmente cualitativa, basada en el análisis de entrevistas en profundidad de profesionales y de relatos de vida de adolescentes complement…

Urban Traffic Analysis through an UAV

2014

Abstract In order to realize a precise and accurate traffic study, a method to evaluate the real traffic flow conditions in urban areas based on videos acquired by an UAV, Unmanned Aerial Vehicle, is proposed. In this way it does not require additional equipment to earth and, not being invasive, does not influence the driver behavior. This paper presents the results obtained in terms of vehicular traffic flow along a way in the city of Palermo. The results have been compared to experiments using macroscopic simulation models and the eventually differences have been discussed.

Traffic fundamentals for A22 Brenner freeway by microsimulation models.

La tesi di dottorato ha avuto come tema lo studio e l’applicazione di un modello di micro-simulazione del traffico in ambito autostradale. Essa si compone di quattro capitoli, con ognuno dei quali si è voluto sintetizzare e descrivere il lavoro di studio e ricerca svolto durante il suddetto corso di Dottorato di Ricerca. L’obiettivo principale del presente lavoro di tesi è stato quello di mettere a punto una metodologia finalizzata all’ottenimento delle relazioni fondamentali di deflusso in ambito autostradale attraverso il software di microsimulazione del traffico Aimsun. Come risulta infatti noto dalla letteratura scientifica, le relazioni fondamentali del deflusso sono utilizzate nel cam…

The stable repression of mesenchymal program is required for hepatocyte identity: A novel role for hepatocyte nuclear factor 4α

2011

The concept that cellular terminal differentiation is stably maintained once development is complete has been questioned by numerous observations showing that differentiated epithelium may undergo an epithelial-to-mesenchymal transition (EMT) program. EMT and the reverse process, mesenchymal-to-epithelial transition (MET), are typical events of development, tissue repair, and tumor progression. In this study, we aimed to clarify the molecular mechanisms underlying these phenotypic conversions in hepatocytes. Hepatocyte nuclear factor 4α (HNF4α) was overexpressed in different hepatocyte cell lines and the resulting gene expression profile was determined by real-time quantitative polymerase…

A complete set of nascent transcription rates for yeast genes

2010

The amount of mRNA in a cell is the result of two opposite reactions: transcription and mRNA degradation. These reactions are governed by kinetics laws, and the most regulated step for many genes is the transcription rate. The transcription rate, which is assumed to be exercised mainly at the RNA polymerase recruitment level, can be calculated using the RNA polymerase densities determined either by run-on or immunoprecipitation using specific antibodies. The yeast Saccharomyces cerevisiae is the ideal model organism to generate a complete set of nascent transcription rates that will prove useful for many gene regulation studies. By combining genomic data from both the GRO (Genomic Run-on) a…

Transcriptional Activity and Nuclear Localization of Cabut, the Drosophila Ortholog of Vertebrate TGF-β-Inducible Early-Response Gene (TIEG) Proteins

2011

Background Cabut (Cbt) is a C2H2-class zinc finger transcription factor involved in embryonic dorsal closure, epithelial regeneration and other developmental processes in Drosophila melanogaster. Cbt orthologs have been identified in other Drosophila species and insects as well as in vertebrates. Indeed, Cbt is the Drosophila ortholog of the group of vertebrate proteins encoded by the TGF-s-inducible early-response genes (TIEGs), which belong to Sp1-like/Kruppel-like family of transcription factors. Several functional domains involved in transcriptional control and subcellular localization have been identified in the vertebrate TIEGs. However, little is known of whether these domains and fu…

Rapid nucleus-scale reorganization of chromatin in neurons enables transcriptional adaptation for memory consolidation

2020

AbstractThe interphase nucleus is functionally organized in active and repressed territories defining the transcriptional status of the cell. However, it remains poorly understood how the nuclear architecture of neurons adapts in response to behaviorally relevant stimuli that trigger fast alterations in gene expression patterns. Imaging of fluorescently tagged nucleosomes revealed that pharmacological manipulation of neuronal activity in vitro and auditory cued fear conditioning in vivo induce nucleus-scale restructuring of chromatin within minutes. Furthermore, the acquisition of auditory fear memory is impaired after infusion of a drug into auditory cortex which blocks chromatin reorganiz…

Expression levels of a filament-specific transcriptional regulator are sufficient to determine Candida albicans morphology and virulence

2009

Candida albicans , the major human fungal pathogen, undergoes a reversible morphological transition from single yeast cells to pseudohyphal and hyphal filaments (elongated cells attached end-to-end). Because typical C. albicans infections contain a mixture of these morphologies it has, for many years, been difficult to assess the relative contribution of each form to virulence. In addition, the regulatory mechanisms that determine growth in pseudohyphal and hyphal morphologies are largely unknown. To address these questions we have generated a C. albicans strain that can be genetically manipulated to grow completely in the hyphal form under non-filament-inducing conditions in vitro. This w…

Regulatory T Cells More Effectively Suppress Th1-Induced Airway Inflammation Compared with Th2

2011

Abstract Asthma is a syndrome with different inflammatory phenotypes. Animal models have shown that, after sensitization and allergen challenge, Th2 and Th1 cells contribute to the development of allergic airway disease. We have previously demonstrated that naturally occurring regulatory T cells (nTregs) can only marginally suppress Th2-induced airway inflammation and airway hyperresponsiveness. In this study, we investigated nTreg-mediated suppression of Th2-induced and Th1-induced acute allergic airway disease. We demonstrate in vivo that nTregs exert their suppressive potency via cAMP transfer on Th2- and Th1-induced airway disease. A comparison of both phenotypes revealed that, despite …

Overexpression of Human and Fly Frataxins in Drosophila Provokes Deleterious Effects at Biochemical, Physiological and Developmental Levels

2011

10 pages, 5 figures. 21779322[PubMed] PMCID: PMC3136927