Search results for " SIL."
showing 10 items of 1630 documents
The Ectocarpus genome and the independent evolution of multicellularity in brown algae
2010
Brown algae (Phaeophyceae) are complex photosynthetic organisms with a very different evolutionary history to green plants, to which they are only distantly related. These seaweeds are the dominant species in rocky coastal ecosystems and they exhibit many interesting adaptations to these, often harsh, environments. Brown algae are also one of only a small number of eukaryotic lineages that have evolved complex multicellularity (Fig. 1). We report the 214 million base pair (Mbp) genome sequence of the filamentous seaweed Ectocarpus siliculosus (Dillwyn) Lyngbye, a model organism for brown algae, closely related to the kelps (Fig. 1). Genome features such as the presence of an extended set of…
Habitat fragmentation and anthropogenic factors affect wildcat Felis silvestris silvestris occupancy and detectability on Mt Etna
2019
International audience; Knowledge of patterns of occupancy is crucial for planning sound biological management and for identifying areas which require paramount conservation attention. The European wildcat Felis silvestris is an elusive carnivore and is classified as ‘least concern' on the IUCN red list, but with a decreasing population trend in some areas. Sicily hosts a peculiar wildcat population, which deserves conservation and management actions, due to its isolation from the mainland. Patterns of occupancy for wildcats are unknown in Italy, and especially in Sicily. We aimed to identify which ecological drivers determined wildcat occurrence on Mt Etna and to provide conservation actio…
RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design.
2011
International audience; Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involv…
Viral fitness determines the magnitude of transcriptomic and epigenomic reprograming of defense responses in plants
2020
Although epigenetic factors may influence the expression of defense genes in plants, their role in antiviral responses and the impact of viral adaptation and evolution in shaping these interactions are still poorly explored. We used two isolates of turnip mosaic potyvirus with varying degrees of adaptation to Arabidopsis thaliana to address these issues. One of the isolates was experimentally evolved in the plant and presented increased load and virulence relative to the ancestral isolate. The magnitude of the transcriptomic responses was larger for the evolved isolate and indicated a role of innate immunity systems triggered by molecular patterns and effectors in the infection process. Sev…
Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance.
2000
AbstractPosttranscriptional gene silencing (PTGS) in plants results from the degradation of mRNAs and shows phenomenological similarities with quelling in fungi and RNAi in animals. Here, we report the isolation of sgs2 and sgs3 Arabidopsis mutants impaired in PTGS. We establish a mechanistic link between PTGS, quelling, and RNAi since the Arabidopsis SGS2 protein is similar to an RNA-dependent RNA polymerase like N. crassa QDE-1, controlling quelling, and C. elegans EGO-1, controlling RNAi. In contrast, SGS3 shows no significant similarity with any known or putative protein, thus defining a specific step of PTGS in plants. Both sgs2 and sgs3 mutants show enhanced susceptibility to virus, d…
Effects of traditional coppice practices and microsite conditions on tree health in a European beech forest at its southernmost range
2016
Abstract: European beech (Fagus sylvatica) grows at the southern limit of its range in the mountain-Mediterranean vegetation belt up to the timberline. The southernmost beech forests of Sicily (southern Italy) show peculiar ecological, structural and silvicultural characteristics, growing in fragmented and isolated stands near the timberline and in topographically marginal unfavorable habitats. Past silvicultural practices increased the heterogeneity of stand structure at these sites. We compared stand structural characteristics and tree health in coppice-cut and control beech stands with respect to the local topographic gradient (bottom, slope and ridge) and canopy cover (clearing/border v…
Reconstruction and analysis of genome-scale metabolic model of a photosynthetic bacterium
2010
Abstract Background Synechocystis sp. PCC6803 is a cyanobacterium considered as a candidate photo-biological production platform - an attractive cell factory capable of using CO2 and light as carbon and energy source, respectively. In order to enable efficient use of metabolic potential of Synechocystis sp. PCC6803, it is of importance to develop tools for uncovering stoichiometric and regulatory principles in the Synechocystis metabolic network. Results We report the most comprehensive metabolic model of Synechocystis sp. PCC6803 available, iSyn669, which includes 882 reactions, associated with 669 genes, and 790 metabolites. The model includes a detailed biomass equation which encompasses…
Record of a 10-year old European Wildcat Felis silvestris silvestris Schreber, 1777 (Mammalia: Carnivora: Felidae) from Mt. Etna, Sicily, Italy
2020
Longevity data for wild felids are lacking in the literature. Here we report a camera trap recapture of a European Wildcat Felis silvestris at Mt. Etna in Sicily, Italy after nine years. This individual was clearly identifiable as its tail ended with a white ring rather than the typical black ring and had a unique shape of the dorsal stripe. At first capture on 26 May 2009, this cat was assessed as an adult, so that the likely minimum age of this individual at the time of recapture on 10 June 2018 must have been be at least 10 years. This finding represents the oldest known European Wildcat in the wild and provides insight into age structure in wildcat populations.
Loading and release of the complex [Pt(DTBTA)(DMSO)Cl]Cl·CHCl3 with the 2,2′-dithiobis(benzothiazole) ligand into mesoporous silica and studies of an…
2018
Abstract Synthetic delivery systems have great potential for overcoming problems associated with systemic toxicity that accompanies chemotherapy with the use of cisplatin and family of platinum anticancer drugs. Mesoporous silicates have been studied in context of drug delivery and drug targeting. In this paper we report the studies of loading and release of a platinum complex, [Pt(DTBTA)(DMSO)Cl]Cl∙CHCl3 (1) where DTBTA = 2,2′-dithiobis(benzothiazole), that was recently synthesized and structurally characterized. Evaluation in vitro of antitumor activity against a human breast cancer cell line (MCF-7) showed a very potent activity of complex(1). Therefore, we thought to incorporate this co…
Design of parallel compensator and stabilizing controller to mitigate non-minimum phase behaviour of the Czochralski Process
2020
Abstract This paper addresses the design of a parallel compensator and a stabilizing controller for the simplified crystal growth dynamics of the Czochralski (CZ) process, i.e., the process for the production of monocrystalline silicon ingots of uniform diameter. The diameter control of the produced ingots is achieved by a CCD camera measurement used to sense the radius of the boundary between the base of the growing crystal and the surrounding glowing meniscus — a raised melt surface connecting the crystal ingot with the flatter melt surface. Due to the intrinsic nature of the process, the bright ring radius measurement signal exhibits a non-minimum phase behaviour. A combination of the pa…