Search results for " Sensitivity"

Characterization of Shiga toxin-producing Escherichia coli O157:H7 isolated in Italy and in France

2002

ABSTRACT Twenty-one Escherichia coli O157:H7 strains isolated in northern Italy from sporadic cases of hemolytic-uremic syndrome and from cattle and food were characterized by virulence gene analysis, pulsed-field gel electrophoresis (PFGE) of Xba I-digested DNA, enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR (ERIC-PCR), and antibiotic resistance patterns and compared to 18 strains isolated in France from human cases of diarrhea, cattle, and the environment. Strains isolated in Sicily (southern Italy) from a local farm (one strain) and from calves just imported from France (11 strains) and Spain (six strains) were also typed. Whereas the eae and hlyA genes were al…

Comparison between Disk Diffusion and Microdilution Methods for Determining Susceptibility of Clinical Fungal Isolates to Caspofungin

2007

ABSTRACT We compared the caspofungin (CAS) susceptibility testing results generated by the disk diffusion (DD) assay with the results of the Clinical and Laboratory Standards Institute (CLSI) broth microdilution (BD) reference method for 106 yeast isolates. The isolates represented 11 different fungal species, including Candida albicans ( n = 50), C. parapsilosis ( n = 10), C. glabrata ( n = 10), C. tropicalis ( n = 10), C. guillermondii ( n = 6), C. rugosa ( n = 5), C. krusei ( n = 5), C. kefyr ( n = 2), C. pelliculosa ( n = 2), Saccharomyces cerevisiae ( n = 3), and Geotrichum candidum ( n = 3). The DD assay was performed in supplemented Mueller-Hinton agar with CAS, which was tested at c…

Evaluation of the Disk Diffusion Method Compared to the Microdilution Method in Susceptibility Testing of Anidulafungin against Filamentous Fungi

2008

ABSTRACT Susceptibility testing of anidulafungin (AFG) against 32 mold isolates showed an excellent correlation between disk diffusion (DD) and broth microdilution methods. Based on our data, a 2-μg disk of AFG and a 24-h reading time might represent the best parameters for AFG DD testing against filamentous fungi.

Class 1 integrons in environmental and clinical isolates of Pseudomonas aeruginosa.

2011

The aims of this study were to ascertain the presence and spread of class 1 integrons amongst environmental and clinical isolates of Pseudomonas aeruginosa and to characterise their variable regions. A total of 76 isolates (56 clinical and 20 environmental) were studied. The presence of plasmids was explored, and polymerase chain reaction (PCR) was used for integron detection. All amplicons were sequenced. PCR detected class 1 integrons in 26 of the 56 clinical isolates; environmental isolates were integron-free. No plasmids were found, thus all the integrons found are possibly on the chromosome. Most isolates presented one amplicon, except PA110514 and PA116136, which showed two PCR produc…

Is there a widespread clone of Serratia marcescens producing outbreaks worldwide?

2021

[Background]: Serratia marcescens frequently causes outbreaks in healthcare settings. There are few studies using high-throughput sequencing (HTS) that analyse S. marcescens outbreaks. We present the analysis of two outbreaks in neonatal intensive care units (NICUs) in hospitals from the Comunitat Valenciana (CV, Spain) and the impact of using different reference genomes.

In vitro activity of LY333328, a new glycopeptide, against extracellular and intracellular vancomycin-resistant enterococci.

2000

The objectives of the study were to observe the activity of LY333328, a new semisynthetic glycopeptide, compared to that of vancomycin against six strains of Enterococcus faecium and Enterococcus faecalis, including four vancomycin-resistant strains. Bacteria ingested by polymorphonuclear leukocytes (PMN) as well as extracellular bacteria were studied using a colony count method. The activity against intracellular bacteria was tested with the drugs present in the extracellular medium, as well as after preincubating the PMN and removal of the drugs. LY333328 is active against the tested enterococci, regardless of their susceptibility to vancomycin, with MICs of 1-2 mg/l. It is bacteriostatic…

Evaluation of microbiological screening in a neonatal intensive care unit to optimize empiric antibiotic use.

2019

Activity against Microorganisms Affecting Cellulosic Objects of the Volatile Constituents of Leonotis nepetaefolia from Nicaragua

2014

The chemical composition of the essential oil from aerial parts of L. nepetaefolia (L.) R. Br. collected in Nicaragua was evaluated by GC and GC-MS. The main components were ( Z)-phytol (22.8%), caryophyllene oxide (18.9%) and hexahydrofarnesylacetone (9.0%). The antibacterial activity against several Gram + and Gram - bacteria, including Bacillus cereus and B. subtilis, both infesting historical cellulosic material, was also determined. B. cereus, B. subtilis and Staphylococcus epidermis were the most affected by the action of the oil.

Accommodation-related changes in monochromatic aberrations of the human eye as a function of age.

2008

PURPOSE. To investigate the relationship between accommodation and the optical aberrations of the whole human eye, as a function of age. METHODS. Sixty healthy subjects with spherical ametropia in the range 3 D, astigmatism less than 1 D, corrected visual acuity of 20/18 or better, and normal findings in an ophthalmic examination were enrolled. Subjects were divided into four groups, with age ranges of 19 to 29, 30 to 39, 40 to 49, and 50 to 60 years. Monochromatic optical aberrations and pupil size were measured with a Hartmann-Shack wavefront sensor under monocular viewing conditions, without pharmacological dilation or cycloplegia. Stimulus vergences were in the range of 0 to 5 D, with a…

Retinal straylight in patients with monofocal and multifocal intraocular lenses

2007

Purpose To determine the differences in retinal straylight perceived by patients with monofocal intraocular lenses (IOLs) and patients with multifocal IOLs. Setting Vissum-Instituto de Oftalmologico de Alicante, Alicante, Spain. Methods In this prospective study, contrast sensitivity and retinal straylight were measured prospectively 6 months postoperatively in 67 eyes of 40 patients with a monofocal or multifocal IOL. In the monofocal group, a ThinOptX IOL (ThinOptX, Inc.) was implanted in 12 eyes and an Acri.Smart 48 S IOL (Acri.Tec) in 20 eyes. In the multifocal IOL group, a ReZoom IOL (Advanced Medical Optics) was implanted in 13 eyes and a ReSTOR IOL (Alcon Laboratories) in 22 eyes. Re…