Search results for " Variation"

The origin of the serpentine endemic Minuartia laricifolia subsp. ophiolitica by vicariance and competitive exclusion.

2013

Serpentine soils harbour a unique flora that is rich in endemics. We examined the evolution of serpentine endemism in Minuartia laricifolia, which has two ecologically distinct subspecies with disjunct distributions: subsp. laricifolia on siliceous rocks in the western Alps and eastern Pyrenees and subsp. ophiolitica on serpentine in the northern Apennines. We analysed AFLPs and chloroplast sequences from 30 populations to examine their relationships and how their current distributions and ecologies were influenced by Quaternary climatic changes. Minuartia laricifolia was divided into four groups with a BAPS cluster analysis of the AFLP data, one group consisted only of subsp. ophiolitica, …

Low diversity but high differentiation: the population genetics of Aglaope infausta (Zygaenidae: Lepidoptera)

2003

Aim Aglaope infausta is a thermophilous Zygaenid of Atlanto-Mediterranean origin, distributed in Portugal, Spain, France and north-western Italy reaching its north-eastern distribution limit in western Germany. The local, regional and inter-regional genetic structure of this species is studied in this analysis. Location and methods  The allozymes of 456 individuals from 12 populations (11 from western Germany and one from southern Portugal) were studied by electrophoresis. Results  Six of the 19 loci analysed were monomorphic. Genetic differentiation between populations was high (FST: 0.404), while the mean genetic diversity was low (He: 3.4%). Most (96.5%) of the genetic variance between p…

Genetic Diversity, Population Size, and Fitness in Central and Peripheral Populations of a Rare Plant Lychnis viscaria

1999

: Genetic diversity is expected to decrease in small and isolated populations as a consequence of bottlenecks, founder effects, inbreeding, and genetic drift. The genetics and ecology of the rare perennial plant Lychnis viscaria (Caryophyllaceae) were studied in both peripheral and central populations within its distribution area. We aimed to investigate the overall level of genetic diversity, its spatial distribution, and possible differences between peripheral and central populations by examining several populations with electrophoresis. Our results showed that the level of genetic diversity varied substantially among populations (  Hexp = 0.000–0.116) and that the total level of genetic …

2005

Background Despite the two-fold cost of sex, most of the higher animals reproduce sexually. The advantage of sex has been suggested to be its ability, through recombination, to generate greater genetic diversity than asexuality, thus enhancing adaptation in a changing environment. We studied the genetic diversity and the population structure of three closely related species of bag worm moths: two strictly sexual (Dahlica charlottae and Siederia rupicolella) and one strictly asexual (D. fennicella). These species compete for the same resources and share the same parasitoids.

Genetic diversity ofNarcissus tortifolius, an endangered endemic species from Southeastern Spain

2015

AbstractIn this work, we analyzed inter-simple sequence repeat markers from 10 populations (298 individuals) spanning the whole distribution range of the endemic Narcissus tortifolius. We assessed genetic variation levels and distribution by estimates of genetic diversity, analysis of molecular variance (AMOVA), principal coordinates and Bayesian methods. N. tortifolius showed moderate genetic diversity at intrapopulation level and low genetic differentiation of populations. In general, Almerian populations showed slightly higher levels of genetic diversity than Murcian populations. Our results indicate that habitat fragmentation has not caused genetic depauperation in N. tortifolius but di…

Genetic diversity and local population structure in Ambrosina bassii (Araceae, Ambrosineae), a Mediterranean relict species

2009

The effects of habitat fragmentation on the genetic structure of Ambrosina bassii are analyzed. The species, whose reproductive biology is mostly unknown, is the only representative of its genus and tribe and it is endemic to the central Mediterranean area. The selected study area was the island of Sicily, in which wild populations show a wide morphological variability and ecological amplitude. Patterns of within- and among-population genetic diversity in eleven Sicilian populations, occurring in six disjunct areas, were examined by means of allozyme electrophoresis. High levels of genetic diversity were found as shown by the mean expected heterozygosity (He ¼ 0.263), the percentage of poly…

Evaluation of intra-cultivar variability in Olea europaea L. cv. Leccino using morphological, biochemical and molecular markers

2020

The phenotypic and genotypic variability of olive tree cv. Leccino (Olea europaea L.), an ancient cultivar from North-Central Italy, was investigated using 44 quantitative and qualitative agro-morphological traits, SDS-PAGE of seed proteins (storage proteins and tegument proteins) and molecular markers such as RAPDs (30 primers) and SSRs (8 primers). Fifteen accessions of Olea europaea catalogued as "Leccino" and one certified tree of "Leccino" (LESt) were examined. The plants were clonally propagated and the measuring of all morphological characteristics was conducted over a period of seven years. The ANOVA analysis showed that all accessions were homogenous for many traits while very few …

Genetic diversity of two perch Perca fluviatilis populations of the Latgale region

2015

Molecular markers based on retrotransposons possibility of integration into genomes of many organisms are commonly used for genetic analysis of different species. The aim of this study was to test possibility of use those markers in perch and detect genetic diversity of populations of two lakes of the Latgale region of Latvia: Cirišs and Sventes. The distance between the lakes is nearly 60 km, they belong to the same Daugava river basin but are different from ecological point of view. Forty two blood samples of <em>Perca fluviatilis </em>were collected altogether. Extracted DNA was analyzed using inter-PBS amplification technique with specifically selected retrotransposon-based …

Isozyme uniformity in a wild extinct insular plant,Lysimachia minoricensisJ.J. Rodr. (Primulaceae)

1999

Isozyme analyses were conducted to evaluate the genetic diversity of seed accessions of Lysimachia minoricensis (a Balearic endemic plant that became extinct in the wild) provided by 10 European botanical gardens. No isozyme variation was detected after examination of the electrophoretic patterns of 22 putative loci in more than 150 plants. The lack of genetic variation in L. minoricensis is probably due to the shortage of sample propagules originally recovered in the field before its extinction. Extant plants of L. minoricensis are believed to have originated from a single source, therefore limiting their use as seed stocks for restoration projects.

Environmental effects on molecular and phenotypic variation in populations of Eruca sativa across a steep climatic gradient

2013

Abstract In Israel Eruca sativa has a geographically narrow distribution across a steep climatic gradient that ranges from mesic Mediterranean to hot desert environments. These conditions offer an opportunity to study the influence of the environment on intraspecific genetic variation. For this, we combined an analysis of neutral genetic markers with a phenotypic evaluation in common-garden experiments, and environmental characterization of populations that included climatic and edaphic parameters, as well as geographic distribution. A Bayesian clustering of individuals from nine representative populations based on amplified fragment length polymorphism (AFLP) divided the populations into a…