Search results for " array"
showing 10 items of 895 documents
Luminometric Sub-nanoliter Droplet-to-droplet Arrays (LUMDA-chip) for Drug Screening on Phase I Metabolism Enzymes
2013
Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for amultiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. themost relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.
Micro and Nano patterns for Biosensing: from enzymatic assays to single cells interaction arrays
2012
In this thesis work, solution dispensing techniques have been employed for the realization of complex biological arrays. Inkjet printing techniques were employed for the generation of drug screening platforms. This approach was initially proved with a model enzyme system like Glucose Oxidase substrate covalently linked to a functionalized silicon oxide support. On this support an enzymatic substrate (D-glucose)/inhibitor (D-glucal) couple was accurately dispensed. A simple optical detection method was used to prove the screening capability of the microarray with the possibility to assay with high reproducibility at the single spot level. Afterwards, this methodology has been extended to CYP…
Genome interdependence in insect-bacterium symbioses
2001
Symbioses between unicellular and multicellular organisms have contributed significantly to the evolution of life on Earth. As exemplified by several studies of bacterium-insect symbioses, modern genomic techniques are providing exciting new information about the molecular basis and the biological roles of these complex relationships, revealing for instance that symbionts have lost many genes for functions that are provided by the host, but that they can provide amino acids that the host cannot synthesize.
Architectural improvements and FPGA implementation of a multimodel neuroprocessor
2003
Since neural networks (NNs) require an enormous amount of learning time, various kinds of dedicated parallel computers have been developed. In the paper a 2-D systolic array (SA) of dedicated processing elements (PEs) also called systolic cells (SCs) is presented as the heart of a multimodel neural-network accelerator. The instruction set of the SA allows the implementation of several neural algorithms, including error back propagation and a self organizing feature map algorithm. Several special architectural facilities are presented in the paper in order to improve the 2-D SA performance. A swapping mechanism of the weight matrix allows the implementation of NNs larger than 2-D SA. A systo…
Integral Imaging Monitors with an Enlarged Viewing Angle
2015
Enlarging the horizontal viewing angle is an important feature of integral imaging monitors. Thus far, the horizontal viewing angle has been enlarged in different ways, such as by changing the size of the elemental images or by tilting the lens array in the capture and reconstruction stages. However, these methods are limited by the microlenses used in the capture stage and by the fact that the images obtained cannot be easily projected into different displays. In this study, we upgrade our previously reported method, called SPOC 2.0. In particular, our new approach, which can be called SPOC 2.1, enlarges the viewing angle by increasing the density of the elemental images in the horizontal …
Microscopic and macroscopic 3D imaging and display by integral imaging
2011
Integral imaging is a rising 3D imaging technique that can be considered the incoherent version of holography. In integral imaging the multiperspective information of 3D scenes is stored in a 2D picture. Such picture, composed by a set of elemental images, is obtained through a 2D array of microlenses. The elemental-images set can be used for many purposes. One is the display of 3D color scenes to audiences or much more than one person. Other is the 3D display, with full parallax, in personal monitors, like the screen of a smartphone, a tablet, or the monitor used by a surgeon in an endoscopic operation. Other important types of applications are connected with the topographic reconstruction…
Reconfigurable electrical interconnection strategies for photovoltaic arrays: A review
2014
Non-uniform irradiance significantly decreases the power delivered by solar photovoltaic arrays. A promising technique for compensating these power losses relies on dynamically reconfiguring the electrical connections between photovoltaic modules. This paper presents the current state-of-the-art strategies for photovoltaic array reconfiguration in order to increase the power output under partial shading and mismatch conditions. The different approaches have been compared in terms of effectiveness of the control algorithms, monitored electrical and environmental variables, overall hardware complexity and specific features of each solution. Finally, the most challenging aspects of the reconfi…
The THESEUS space mission concept: science case, design and expected performances
2018
THESEUS is a space mission concept aimed at exploiting Gamma-Ray Bursts for investigating the early Universe and at providing a substantial advancement of multi-messenger and time-domain astrophysics. These goals will be achieved through a unique combination of instruments allowing GRB and X-ray transient detection over a broad field of view (more than 1sr) with 0.5¿1 arcmin localization, an energy band extending from several MeV down to 0.3¿keV and high sensitivity to transient sources in the soft X-ray domain, as well as on-board prompt (few minutes) follow-up with a 0.7¿m class IR telescope with both imaging and spectroscopic capabilities. THESEUS will be perfectly suited for addressing …
Artemisinin derivatives induce iron-dependent cell death (ferroptosis) in tumor cells
2015
Abstract Background Apoptosis and other forms of cell death have been intensively investigated in the past years to explain the mode of action of synthetic anticancer drugs and natural products. Recently, a new form of cell death emerged, which was termed ferroptosis, because it depends on intracellular iron. Here, the role of genes involved in iron metabolism and homeostasis for the cytotoxicity of ten artemisinin derivatives have been systematically investigated. Material and methods Log10IC50 values of 10 artemisinin derivatives (artesunate, artemether, arteether, artenimol, artemisitene, arteanuin B, another monomeric artemisinin derivative and three artemisinin dimer molecules) were co…
Changes in the protein profile of Quercus ilex leaves in response to drought stress and recovery
2009
To characterize the molecular response of holm oak to drought stress and its capacity to recover 9-month-old Quercus ilex seedlings were subjected to three treatments for a 14-d period: (i) continuous watering to field capacity (control plants, W), (ii) no irrigation (drought treatment, D), and (iii) no irrigation for 7d followed by a watering period of 7d (recovery treatment, R). In drought plants, leaf water potential decreased from -0.72 (day 0) to -0.99MPa (day 7), and -1.50MPa (day 14). Shoot relative water content decreased from 49.3% (day 0) to 47.7% (day 7) and 40.8% (day 14). Photosystem II quantum yield decreased from 0.80 (day 0) to 0.72 (day 7) and 0.73 (day 14). Plants subjecte…