Search results for " assembly."

Self-assembly process of diimidazolium salts: the effect of the alkyl chain length and isomeric substitution

Palermo si cura. Pratiche culturali di mutuo soccorso come risposta alle fragilità della città (post)pandemica

2023

In un quadro di riferimento in cui la crisi è globalizzata ma diseguale e in mancanza di un sistema di welfare capace di rispondere alle urgenze dei contesti e dei gruppi sociali più marginalizzati, una risposta ai nuovi bisogni sociali ed individuali è stata offerta da reti solidali e da pratiche di mobilitazione dal basso che svolgono un ruolo di agency territoriale, spesso indipendentemente o in assenza di Istituzioni. Nel contesto palermitano un impegno agito da una pluralità di soggetti culturali già attivi sul territorio, riuniti in un’assemblea pubblica (Palermo si cura), durante il periodo del primo lockdown hanno dato vita ad azioni di mutuo soccorso per rispondere all’emergenza ec…

Vittorio Emanuele Orlando. Il giurista, l’avvocato, lo statista

2018

Vittorio Emanuele Orlando is one of the defining figures of the history of Italy. Lawyer, jurist and statesman, he founded the Italian School of Public Law and worked out an original version of the “Rechtsstaat”. He led Italy to victory during the First World War, took then an official stance against the Fascism and played a significant role in the republican constituent process. The article suggests a coherent interpretation of the several aspects of his personality and provides an extensive bibliography

Efficient DNA Packaging of Bacteriophage PRD1 Requires the Unique Vertex Protein P6

2007

ABSTRACT The assembly of bacteriophage PRD1 proceeds via formation of empty procapsids containing an internal lipid membrane, into which the linear double-stranded DNA genome is subsequently packaged. The packaging ATPase P9 and other putative packaging proteins have been shown to be located at a unique vertex of the PRD1 capsid. Here, we describe the isolation and characterization of a suppressor-sensitive PRD1 mutant deficient in the unique vertex protein P6. Protein P6 was found to be an essential part of the PRD1 packaging machinery; its absence leads to greatly reduced packaging efficiency. Lack of P6 was not found to affect particle assembly, because in the P6-deficient mutant infecti…

Self-assembly and intra-cluster reactions of erbium and ytterbium bis(2-ethylhexyl)sulfosuccinates in the gas phase.

2014

RATIONALE: The study of surfactant organization in vacuum allows surfactant–surfactant interaction to be unveiled in the absence of surrounding solvent molecules. Knowledge on their chemical-physical properties may also lead to the definition of more efficient gas-phase carriers, air-cleaning agents and nanoreactors. In addition, the presence of lanthanide-group ions adds unique photochemical properties to surfactants. METHODS: The structural features, stability and fragmentation patterns of charged aggregates formed by lanthanide- functionalized surfactants, ytterbium and erbium bis(2-ethylhexyl)sulfosuccinate ((AOT) 3 Yb and (AOT) 3 Er), have been investigated by electrospray ionization m…

Musket: a multistage k-mer spectrum-based error corrector for Illumina sequence data

2012

Abstract Motivation: The imperfect sequence data produced by next-generation sequencing technologies have motivated the development of a number of short-read error correctors in recent years. The majority of methods focus on the correction of substitution errors, which are the dominant error source in data produced by Illumina sequencing technology. Existing tools either score high in terms of recall or precision but not consistently high in terms of both measures. Results: In this article, we present Musket, an efficient multistage k-mer-based corrector for Illumina short-read data. We use the k-mer spectrum approach and introduce three correction techniques in a multistage workflow: two-s…

CARE: context-aware sequencing read error correction.

2020

Abstract Motivation Error correction is a fundamental pre-processing step in many Next-Generation Sequencing (NGS) pipelines, in particular for de novo genome assembly. However, existing error correction methods either suffer from high false-positive rates since they break reads into independent k-mers or do not scale efficiently to large amounts of sequencing reads and complex genomes. Results We present CARE—an alignment-based scalable error correction algorithm for Illumina data using the concept of minhashing. Minhashing allows for efficient similarity search within large sequencing read collections which enables fast computation of high-quality multiple alignments. Sequencing errors ar…

Analysis of the thermomechanical behavior of the IFMIF bayonet target assembly under design loading scenarios

2015

In the framework of the IFMIF Engineering Validation and Engineering Design Activities (IFMIF/EVEDA) phase, ENEA is responsible for the design of the European concept of the IFMIF lithium target system which foresees the possibility to periodically replace only the most irradiated and thus critical component (i.e., the backplate) while continuing to operate the rest of the target for a longer period (the so-called bayonet backplate concept). In this work, the results of the steady state thermomechanical analysis of the IFMIF bayonet target assembly under two different design loading scenarios (a "hot" scenario and a "cold" scenario) are briefly reported highlighting the relevant indications…

On the thermomechanical behavior of the European target assembly design of IFMIF-EVEDA lithium test loop under start-up transient scenarios

2013

Uncoupled thermomechanical transient analyses have been carried out to investigate the behavior of IFMIF-EVEDA lithium test loop bayonet backplate target assembly under two selected start-up transient operational scenarios. The first transient scenario considered foresees that the target assembly, starting from the initial uniform temperature of 50 C, is heated up uniquely by convective heat transfer with lithium, flowing from inlet to outlet nozzle at its reference nominal temperature and pressure, until its nominal steady state thermal field distribution is reached. The second transient scenario foresees, more realistically, that the target assembly, starting from the uniform temperature …

Study of the thermo-mechanical performances of the IFMIF-EVEDA Lithium Test Loop target assembly

2012

Abstract Within the framework of the IFMIF R&D program and in close cooperation with ENEA-Brasimone, at the Department of Energy of the University of Palermo a research campaign has been launched to investigate the thermo-mechanical behavior of the target assembly under both steady state and start-up transient conditions. A theoretical approach based on the finite element method (FEM) has been followed and a well-known commercial code has been adopted. A realistic 3D FEM model of the target assembly has been set-up and optimized by running a mesh independency analysis. A proper set of loads and boundary conditions, mainly concerned with radiation heat transfer between the target assembly ex…