Search results for " frames"
showing 10 items of 175 documents
Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracel…
2010
Abstract Background A bacterial artificial chromosomal library of Planobispora rosea, a genetically intractable actinomycete strain, was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of genes known to be involved in the biosynthesis of antibiotics. Results One clone with a 40 kb insert showed antimicrobial activity against Gram positive bacteria. Insert sequence analysis and subcloning experiments revealed that the bioactivity was due to a 3.5 kb DNA fragment containing two open reading frames. These orfs encode two proteins with high similarity to a putative membrane protein of Streptomyces coelicolor and to the nogalamycin resis…
GenClust: A genetic algorithm for clustering gene expression data
2005
Abstract Background Clustering is a key step in the analysis of gene expression data, and in fact, many classical clustering algorithms are used, or more innovative ones have been designed and validated for the task. Despite the widespread use of artificial intelligence techniques in bioinformatics and, more generally, data analysis, there are very few clustering algorithms based on the genetic paradigm, yet that paradigm has great potential in finding good heuristic solutions to a difficult optimization problem such as clustering. Results GenClust is a new genetic algorithm for clustering gene expression data. It has two key features: (a) a novel coding of the search space that is simple, …
A longitudinal examination of the transition to symbolic communication in the second year of life
2003
Between 10 and 24 months of age, children progress from communicating through conventional signals to communicating through symbols in a variety of situations. The present study investigates this transition analysing mother–child communication frames and the child’s communicative acts, and tracing the developmental changes in both frames and communicative acts. Four children (2 girls, 2 boys) and their mothers were observed longitudinally and extensively, from 10 to 24 months of age, using a multiple case-study method. Through the detailed investigation of these single cases, clear developmental trajectories were found, showing that conventional frames and representational gestures ‘bridge’…
Seismic Performance of Earthquake-Resilient RC Frames Made with HSTC Beams and Friction Damper Devices
2021
Seismic behavior of RC frames with hybrid steel-trussed concrete beams is affected by panel zone damage due to a large amount of longitudinal reinforcement. Here the seismic efficiency of innovative frames characterized by friction damper devices (FDDs) at beam-to-column connections is compared against traditional frame. Three configurations are investigated: FDDs alone; FDDs with column-to-foundation connections having preloaded threaded bars and disk springs; FDDs with self-centering friction devices. Non-linear analyses show that FDDs alone prevent plastic hinge formation at beam ends and beam–column joint damage. FDDs with self-centering friction devices effectively limit both peak and …
Orbits of bounded bijective operators and Gabor frames
2020
This paper is a contribution to frame theory. Frames in a Hilbert space are generalizations of orthonormal bases. In particular, Gabor frames of $L^2(\mathbb{R})$, which are made of translations and modulations of one or more windows, are often used in applications. More precisely, the paper deals with a question posed in the last years by Christensen and Hasannasab about the existence of overcomplete Gabor frames, with some ordering over $\mathbb{Z}$, which are orbits of bounded operators on $L^2(\mathbb{R})$. Two classes of overcomplete Gabor frames which cannot be ordered over $\mathbb{Z}$ and represented by orbits of operators in $GL(L^2(\mathbb{R}))$ are given. Some results about opera…
Do metric fluctuations affect the Higgs dynamics during inflation?
2017
We show that the dynamics of the Higgs field during inflation is not affected by metric fluctuations if the Higgs is an energetically subdominant light spectator. For Standard Model parameters we find that couplings between Higgs and metric fluctuations are suppressed by $\mathcal{O}(10^{-7})$. They are negligible compared to both pure Higgs terms in the effective potential and the unavoidable non-minimal Higgs coupling to background scalar curvature. The question of the electroweak vacuum instability during high energy scale inflation can therefore be studied consistently using the Jordan frame action in a Friedmann--Lema\^itre--Robertson--Walker metric, where the Higgs-curvature coupling …
Prediction of the out-of-plane response of infilled frames under seismic loads by a new fiber-section macro-model
2016
This chapter suggests that an extension of the capability of the traditional inplane macro-models to capture the simultaneous in-plane and Out-Of-Plane (OOP) response of infills. A new simplified macro-model for the assessment of both in plane and out-of-plane responses of infilled frames was developed and validated. The possibility to simulate the arching action is achieved by using distributed plasticity fiber-section elements, able to directly account for the coupling between axial load and bending moment. The arching action is explicitly introduced by the use of fiber-section beam-column elements, which can model the coupling between axial-load and bending moment. The model presented is…
The frontier between cell and organelle: genome analysis of Candidatus Carsonella ruddii
2007
Background Bacterial symbioses are widespread among insects. The early establishment of such symbiotic associations has probably been one of the key factors for the evolutionary success of insects, since it may have allowed access to novel ecological niches and to new imbalanced food resources, such as plant sap or blood. Several genomes of bacterial endosymbionts of different insect species have been recently sequenced, and their biology has been extensively studied. Recently, the complete genome sequence of Candidatus Carsonella ruddii, considered the primary endosymbiont of the psyllid Pachpsylla venusta, has been published. This genome consists of a circular chromosome of 159,662 bp and…
Cloning, deletion, and characterization of PadR, the transcriptional repressor of the phenolic acid decarboxylase-encoding padA gene of Lactobacillus…
2004
ABSTRACTLactobacillus plantarumdisplays a substrate-induciblepadAgene encoding a phenolic acid decarboxylase enzyme (PadA) that is considered a specific chemical stress response to the inducing substrate. The putative regulator ofpadAwas located in thepadAlocus based on its 52% identity with PadR, thepadAgene transcriptional regulator ofPediococcus pentosaceus(L. Barthelmebs, B. Lecomte, C. Diviès, and J.-F. Cavin, J. Bacteriol.182:6724-6731, 2000). Deletion of theL. plantarum padRgene clearly demonstrates that the protein it encodes is the transcriptional repressor of divergently orientedpadA. ThepadRgene is cotranscribed with a downstream open reading frame (ORF1), the product of which m…
Cloning and characterization of the genes encoding the malolactic enzyme and the malate permease of Leuconostoc oenos
1996
Using degenerated primers from conserved regions of the protein sequences of malic enzymes, we amplified a 324-bp DNA fragment by PCR from Leuconostoc oenos and used this fragment as a probe for screening a Leuconostoc oenos genomic bank. Of the 2,990 clones in the genomic bank examined, 7 with overlapping fragments were isolated by performing colony hybridization experiments. Sequencing 3,453 bp from overlapping fragments revealed two open reading frames that were 1,623 and 942 nucleotides long and were followed by a putative terminator structure. The first deduced protein (molecular weight, 59,118) is very similar (level of similarity, 66%) to the malolactic enzyme of Lactococcus lactis; …