Search results for " medium"

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

2017

Growth phenotypes of microorganisms are a strong indicator of their underlying genetic fitness and can be segregated into 3 growth regimes: lag-phase, log-phase, and stationary-phase. Each growth phase can reveal different aspects of fitness that are related to various environmental and genetic conditions. High-resolution and quantitative measurements of all 3 phases of growth are generally difficult to obtain. Here we present a detailed method to characterize all 3 growth phases on solid media using an assay called One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). ODELAY quantifies growth phenotypes of individual cells growing into colonies on solid media using time-lapse mi…

Modeling mass transfer in fracture flows with the time domain-random walk method

2019

The time domain-random walk method was developed further for simulating mass transfer in fracture flows together with matrix diffusion in surrounding porous media. Specifically, a time domain-random walk scheme was developed for numerically approximating solutions of the advection-diffusion equation when the diffusion coefficient exhibits significant spatial variation or even discontinuities. The proposed scheme relies on second-order accurate, central-difference approximations of the advective and diffusive fluxes. The scheme was verified by comparing simulated results against analytical solutions in flow configurations involving a rectangular channel connected on one side with a porous ma…

Partial purification and characterization of an NAD-dependent 3 beta-hydroxysteroid dehydrogenase from Clostridium innocuum

1989

In nine strains of Clostridium innocuum, 3 beta-hydroxysteroid-dehydrogenating activities were detected. 3 beta, 7 alpha, 12 alpha-Trihydroxy- and 3 beta-hydroxy-12-keto-5 beta-cholanoic acids were identified as reduction products of the respective 3-keto bile acids by gas-liquid chromatography and gas-liquid chromatography-mass spectrometry. One strain was shown to contain a NAD-dependent 3 beta-hydroxysteroid dehydrogenase. Enzyme production was constitutive in the absence of added bile acids. The specific enzyme activity was significantly reduced by growth medium supplementation with 3-keto bile acids, with trisubstituted acids being more effective than disubstituted ones. A pH optimum o…

Nanomechanics of individual aerographite tetrapods

2017

Carbon-based three-dimensional aerographite networks, built from interconnected hollow tubular tetrapods of multilayer graphene, are ultra-lightweight materials recently discovered and ideal for advanced multifunctional applications. In order to predict the bulk mechanical behaviour of networks it is very important to understand the mechanics of their individual building blocks. Here we characterize the mechanical response of single aerographite tetrapods via in situ scanning electron and atomic force microscopy measurements. To understand the acquired results, which show that the overall behaviour of the tetrapod is governed by the buckling of the central joint, a mechanical nonlinear mode…

Thermomineral waters of Greece: geochemical characterization

2020

Permeability of three-dimensional random fiber webs

1998

We report the results of essentially ab initio simulations of creeping flow through large threedimensional random fiber webs that closely resemble fibrous sheets such as paper and nonwoven fabrics. The computational scheme used in this Letter is that of the lattice-Boltzmann method and contains no free parameters concerning the properties of the porous medium or the dynamics of the flow. The computed permeability of the web is found to be in good agreement with experimental data, and confirms that permeability depends exponentially on porosity over a large range of porosity. [S0031-9007(97)05087-4]

Giant hepatocellular adenoma as cause of severe abdominal pain: a case report

2007

Abstract The authors describe the case of a large hepatocellular adenoma diagnosed in a 30-year old woman who came to us complaining of acute pain in the upper abdominal quadrants. The patient had been taking an oral contraceptive pill for the last ten years. We present the clinical features, the diagnostic work-up and the treatment prescribed.

An Introduction to Soil and Water Infiltration

2016

This chapter provides a short presentation of general characteristics of the soil. Basic concepts concerning soil water content and potential and flow of water in the porous medium are then reported since these concepts are diffusely used throughout this book. The infiltration process is finally illustrated considering different modeling approaches. In particular, development of analytical infiltration models is described with reference to one-dimensional gravity-free water absorption, one-dimensional gravity driven infiltration, one-dimensional gravity and capillary driven infiltration and, finally, three-dimensional gravity and capillary driven infiltration.

X-ray CT imaging as a scientific tool to study the capillary water absorption in sedimentary rocks used in cultural heritages

2009

This paper proposes the X-Ray CT imaging as appropriate tool for investigating the capillary water absorption in sedimentary rocks. This technique, in fact, provides information useful for deeping the knowledge about of the porosity and the kinetics of the water capillary absorption in porous materials. The possibility to improve in non invasive manner, the understanding of this phenomenon, constitutes a fundamental aspect to take actions in the restoration and conservation of lapideous artifact and monuments from cultural heritages. The investigated sedimentary rocks come from different Sicilian quarries and were used for the building of the Greek temples in the archaeological areas of Agr…

Dioxouranium(VI) hydrolysis at 75 and 100°C in 3.6 molkg-1 LiClO4

2008

This work concerns the acidic properties of uranyl ion UO22+ at 75 and 100 °C in 3.6 molal (molkg-1 solvent) LiClO4 aqueous medium. The investigation was carried out by a coulometric-potentiometric technique. Direct and reverse acid base titrations were carried out in order to check the achievement of actual equilibrium conditions. Moreover, in order to reveal oversaturation conditions, a further check was carried out on clean saturated hydrolyzed solutions.