Search results for " method"
showing 10 items of 10455 documents
Soil erosion in sloping vineyards assessed by using botanical indicators and sediment collectors in the Ruwer-Mosel valley
2016
Steep slopes, erodible soils, rill and ephemeral gullies, compaction due to wheel. traffic and human trampling are common features in vineyards around the world and result in high soil erosion rates. However, little is known about seasonal and spatial variations of soil erosion rates due to factors such as the impact of the vine plantation, harvest, and tillage on the soil redistribution over the long-term temporal scale. The goal of this study is to assess long-term soil erosion rates and the impact of management on sediment and runoff yield by means of Gerlach troughs and a topographical approach based on botanic benchmarks in two paired vineyards with different ages (3 and 35 years) loca…
First M87 Event Horizon Telescope Results. III. Data Processing and Calibration
2019
We present the calibration and reduction of Event Horizon Telescope (EHT) 1.3 mm radio wavelength observations of the supermassive black hole candidate at the center of the radio galaxy M87 and the quasar 3C 279, taken during the 2017 April 5–11 observing campaign. These global very long baseline interferometric observations include for the first time the highly sensitive Atacama Large Millimeter/submillimeter Array (ALMA); reaching an angular resolution of 25 μas, with characteristic sensitivity limits of ~1 mJy on baselines to ALMA and ~10 mJy on other baselines. The observations present challenges for existing data processing tools, arising from the rapid atmospheric phase fluctuations, …
First M87 Event Horizon Telescope Results. II. Array and Instrumentation
2019
The Event Horizon Telescope (EHT) is a very long baseline interferometry (VLBI) array that comprises millimeter- and submillimeter-wavelength telescopes separated by distances comparable to the diameter of the Earth. At a nominal operating wavelength of ~1.3 mm, EHT angular resolution (λ/D) is ~25 μas, which is sufficient to resolve nearby supermassive black hole candidates on spatial and temporal scales that correspond to their event horizons. With this capability, the EHT scientific goals are to probe general relativistic effects in the strong-field regime and to study accretion and relativistic jet formation near the black hole boundary. In this Letter we describe the system design of th…
Time domain astronomy with the THESEUS satellite
2021
THESEUS is a medium size space mission of the European Space Agency, currently under evaluation for a possible launch in 2032. Its main objectives are to investigate the early Universe through the observation of gamma-ray bursts and to study the gravitational waves electromagnetic counterparts and neutrino events. On the other hand, its instruments, which include a wide field of view X-ray (0.3-5 keV) telescope based on lobster-eye focussing optics and a gamma-ray spectrometer with imaging capabilities in the 2-150 keV range, are also ideal for carrying out unprecedented studies in time domain astrophysics. In addition, the presence onboard of a 70 cm near infrared telescope will allow simu…
Pleistocene paleoenvironmental reconstructions and mammalian evolution in South-East Asia: focus on fossil faunas from Thailand.
2006
16 pages; International audience; Mammalian faunal studies have provided various clues for a better reconstruction of hominid Quaternary paleoenvironments. Inthis work, two methods were used: (1) the cenogram method, based on a graphical representation of the mammalian communitystructure, and (2) the species richness of murine rodents to estimate climatic parameters. These methods were applied to Middle andLate Pleistocene mammalian faunas of South-East Asia, from South China to Indonesia. Special emphasis was laid on a fauna fromnorth-east Thailand dated back to approximately 170,000 years (i.e. a glacial period). This Thai fauna seems characteristic of aslightly open forested environment …
Patch-based survey methods for studying prehistoric human land-use in agriculturally modified landscapes: A case study from the Canal de Navarrés, ea…
2018
Abstract In landscapes whose surface has been modified by terracing and other agricultural land-use, the spatial and temporal patterning of prehistoric settlement can be difficult to detect using traditional, site-orientated archaeological survey methods, especially for small-scale societies. In these contexts, methods that can reveal occupational patterns at landscape scales, without the need to pinpoint specific sites of human occupation, can be especially useful. We employ a stratified, randomly selected patch-based survey strategy to examine socio-ecological dynamics from the Middle Paleolithic through Bell Beaker (Chalcolithic) periods within the Canal de Navarres, eastern Spain. We di…
Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine
2017
Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expres…
Ocean acidification at a coastal CO2 vent induces expression of stress-related transcripts and transposable elements in the sea anemone Anemonia viri…
2019
Published version, available at: https://doi.org/10.1371/journal.pone.0210358 Ocean acidification threatens to disrupt interactions between organisms throughout marine ecosystems. The diversity of reef-building organisms decreases as seawater CO2 increases along natural gradients, yet soft-bodied animals, such as sea anemones, are often resilient. We sequenced the polyA-enriched transcriptome of adult sea anemone Anemonia viridis and its dinoflagellate symbiont sampled along a natural CO2 gradient in Italy to assess stress levels in these organisms. We found that about 1.4% of the anemone transcripts, but only ~0.5% of the Symbiodinium sp. transcripts were differentially expressed. Processe…
Give me a sample of air and I will tell which species are found from your region : Molecular identification of fungi from airborne spore samples
2017
Fungi are a megadiverse group of organisms, they play major roles in ecosystem functioning and are important for human health, food production and nature conservation. Our knowledge on fungal diversity and fungal ecology is however still very limited, in part because surveying and identifying fungi is time demanding and requires expert knowledge. We present a method that allows anyone to generate a list of fungal species likely to occur in a region of interest, with minimal effort and without requiring taxonomical expertise. The method consists of using a cyclone sampler to acquire fungal spores directly from the air to an Eppendorf tube, and applying DNA barcoding with probabilistic specie…
Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology
2020
In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview r…