Search results for " microbiologia"

Investigation and control of a Norovirus outbreak of probable waterborne transmission through a municipal groundwater system

2014

During March 2011 an outbreak of gastroenteritis occurred in Santo Stefano di Quisquina, Agrigento, Sicily, Italy. Within two weeks 156 cases were identified among the 4,965 people living in the municipality. An epidemiological investigation was conducted to characterize the outbreak and target the control measures. A case was defined as a person developing diarrhea or vomiting during February 27–March 13, 2011. Stool specimens were collected from 12 cases. Norovirus (NoV) genotype GII.4 variant New Orleans 2009 was identified in stool samples from 11 of 12 cases tested (91.7%). Epidemiological investigations suggested a possible association with municipal drinking water consumption. Water …

The influence of some dietary components on intestinal microbiota

2016

The gut microbiota is the set of symbiotic microorganisms that resides in our intestine. The interaction between the gut microbiota and the mucosal immune system can be altered as a result of shifts in the composition or metabolic activity of the intestinal microflora. Recent studies establish that diet is one of the main involved factors in determining the microbial composition of the gut suggesting its role as external factor able to promote the onset of specific diseases by disrupting the immune homeostasis. Starting from the evidence that the 57% of the gut microbiota’s entire variation are due to dietary alterations this review aims at providing an overview of the positive impact of so…

Response to salinity stress of Rhizobium leguminosarum bv. viciae strains in the presence of different legume host plants

2011

We investigated the effect of residual salts from the previous summer’s irrigation on two non-irrigated cover crops—broad bean and common vetch—and on their rhizobial symbiontics. Before sowing, seeds were inoculated with a salt-tolerant strain and a salt-sensitive strain of Rhizobium leguminosarum biovar viciae. An increase in the electrical conductivity of the saturated-soil extract from 2.0 dS m−1 to 6.0 dS m−1 caused a severe reduction of broad bean biomass, while growth of common vetch was almost unaffected by the salinity level. Our results clearly indicate that common vetch as a cover crop may increase the availability of nitrogen in soil more than broad bean also in saline environme…

Presence of enterococci in raw cow’s milk and “Puzzone di Moena” cheese

2009

Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

2020

The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five Listeria monocytogenes isolates from a variety of foods and five Escherichia coli isolates from a farm. The E. coli isolates were positive for extended spectrum &beta

Biofilm capability of staphylococcus strains isolated from food and the anti-biofilm activity of a chemically synthesized pyrrolomycin

2018


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Involvement of an Alkane Hydroxylase System of Gordonia sp. Strain SoCg in Degradation of Solid n-Alkanes▿

2010

ABSTRACT Enzymes involved in oxidation of long-chain n -alkanes are still not well known, especially those in Gram-positive bacteria. This work describes the alkane degradation system of the n -alkane degrader actinobacterium Gordonia sp. strain SoCg, which is able to grow on n -alkanes from dodecane (C 12 ) to hexatriacontane (C 36 ) as the sole C source. SoCg harbors in its chromosome a single alk locus carrying six open reading frames (ORFs), which shows 78 to 79% identity with the alkane hydroxylase (AH)-encoding systems of other alkane-degrading actinobacteria. Quantitative reverse transcription-PCR showed that the genes encoding AlkB (alkane 1-monooxygenase), RubA3 (rubredoxin), RubA4…

The influence of Opuntia ficus-indica mucilage edible coating on the quality of ‘Hayward’ kiwifruit slices

2016

Abstract The aim of this work was to study the effect of mucilage edible coating extracted from Opuntia ficus-indica (OFI) on the quality and shelf life maintenance of packaged kiwifruit slices. OFI mucilage alone or added with TWEEN ® 20 were applied on kiwifruit fresh cut surfaces. After treatments, kiwifruit samples were stored under passive atmosphere at 5 ± 1 °C for 3, 5, 7 and 12 days. At each storage period, visual quality and flavor score, pectin content, ascorbic acid and the microbiological characteristics were measured together with CO 2 and O 2 content in the packages. Kiwifruit slices coated only with mucilage or with mucilage plus Tween 20, showed a significant higher firmness…

Isolation of Gram-positive n-alkane degraders from a hydrocarbon-contaminated Mediterranean shoreline.

2007

Aims: To investigate the petroleum hydrocarbon (HC)-degrading potential of indigenous micro-organisms in a sandy Mediterranean coast, accidentally contaminated with petroleum-derived HCs. Methods and Results: Using culturable methods, a population of Gram-positive n-alkane degraders was detected in the contaminated soil. Five isolates, identified as one Nocardia, two Rhodococcus and two Gordonia strains, were able to degrade medium- and long-chain n-alkanes up to C36 as assessed by growth assays and gas chromatography-mass spectrometry analysis. Diverging alkane hydroxylase-encoding genes (alkB) were detected by PCR, using degenerated primers, in all the strains; multiple sequences were obt…

Investigation of the hygienic safety of aromatic plants cultivated in soil contaminated with Listeria monocytogenes

2012

Abstract The present work was undertaken to investigate the survival of Listeria monocytogenes ATCC 19114T in soil during the whole crop cycle of rocket (Eruca sativa Mill.) and basil (Ocimum basilicum L.), to monitor its transfer to the leaves, and to evaluate its viability at harvest. To this purpose, the soil was N–P–K fertilized and four trials, obtained with different combinations of soil treatment, listerial inoculums and seed planting, were followed for each aromatic plant. Soil was weekly investigated for total microbial counts and L. monocytogenes evolution. At the starting time, un-inoculated autoclaved soil showed a limited microbial load (103 CFU g dw−1), while un-inoculated non…