Search results for " orange"
showing 10 items of 99 documents
Identification of inorganic pigments from paintings and polychromed sculptures immobilized into polymer film electrodes by stripping differential pul…
2000
Abstract Inorganic pigments in paintings and polychromed sculptures are studied by cyclic voltammetry and differential pulse stripping voltammetry using micro-sample coatings in Paraloid B72-film modified electrodes. Characteristic cathodic and anodic differential pulse profiles were obtained in the +0.4 to −1.0 V vs. SCE potential range for different cadmium, copper, lead, mercury and zinc pigments used in traditional colour palettes. Under optimized conditions, excellent reproducibility was obtained. Microsamples extracted from polychromed sculptures, wall paintings, canvas paintings, panel paintings and altarpieces from Spain, Ethiopia and Italy from the 12th to the 20th centuries have b…
Aspidin PB, a phloroglucinol derivative, induces apoptosis in human hepatocarcinoma HepG2 cells by modulating PI3K/Akt/GSK3β pathway.
2012
Aspidin PB, a phloroglucinol derivative isolated from Dryopteris fragrans (L.) Schott, has been previously reported to exert high biological activities. In the present study, we analyzed the apoptotic mechanisms of aspidin PB on human hepatoma cell line, HepG2. Initially, aspidin PB was shown to inhibit the growth of HepG2 cells in a time and dose-dependent manner. After treatment with aspidin PB for 72 h, 48 h and 24 h using MTT assay, the IC(50) values were 10.59 μM, 20.86 μM and 46.59 μM, respectively. Aspidin PB was capable to induce apoptosis, as measured by mitochondrial membrane potential (ΔΨm), acridine orange (AO) staining and propidium iodide (PI)/annexin V-FITC double staining. T…
Anti-inflammatory effects of chemically modified tetracyclines by the inhibition of nitric oxide and interleukin-12 synthesis in J774 cell line
2001
We investigated the effects of chemically modified tetracyclines (CMTs) on the production of nitric oxide (NO) and on the synthesis of some cytokines: tumour necrosis factor alpha (TNF-alpha), interleukin(IL)-10 and IL-12 in lipopolysaccharide (LPS)-treated J774 cell line. Furthermore, we studied the ability of these drugs to modify the viability in LPS-stimulated J774 macrophages. CMTs decreased, in a dose-dependent manner, inducible NO synthase (iNOS) activity and, consequently, nitrite formation in J774 cultures. The CMT-induced decrease in NO production is due to the inhibition of enzyme activity rather than to a direct effect on enzyme expression. The absence of the inhibition in mRNA …
The Orange Peel: An Outstanding Source of Chemical Resources
2021
Citrus sinensis (L.) Osbeck is a very common cultivar belonging to the Rutaceae family. It is largely diffused in several areas of the world characterized by mild to warm climate conditions. Its abundant worldwide production (up to 107 Tons. per year) and consumption both as the edible part of the fruit and as several types of derivative products imply the production of a huge amount of waste, such as the fruit pomace. Several ways of recycling this material have been developed in recent years: employment as fertilizer, fodder ingredient, and even cloth material. However, the chemical added value of Citrus sinensis peel has been underestimated despite the diversified and significant content…
Abatement of Acid Orange 7 in macro and micro reactors. Effect of the electrocatalytic route
2014
Abstract The electrochemical treatment of aqueous solutions contaminated by Acid Orange 7 (AO7) was widely studied with the main objective to evaluate as the electrocatalytic route affects the performances of the degradation process in macro and microfluidic cells. Direct anodic oxidation (EO), electro-Fenton (EF), electro-generation of active chlorine (IOAC) and coupled processes were investigated in macro and microfluidic reactors in order to select more effective conditions for the treatment of such compound. The effect of numerous operative parameters (such as nature of the electrode materials, coupling of processes, flow rate, current density and inter-electrode distance) on the perfor…
Flow-cytometric enumeration of reticulocytes with the new fluorochrome 1′,3′-diethyl-4,2′-quinolylthiacyanine
1992
Several flow-cytometric methods for reticulocyte enumeration in whole blood have been developed, with different degrees of practical use. Recently, a new fluorochrome, 1′,3′-diethyl-4,2′-quinolylthyacianine (DEQTC) was proposed in a brief report, as an alternative to thiazole orange for reticulocyte counting. We have evaluated the usefulness of this fluorescent stain by assessing the optimal conditions for the flow-cytometric analysis, and by comparing in double-blind assays the quantitative results of this technique with those obtained by manual counting with brilliant cresyl blue. Our results show that flow cytometry with DEQTC is highly correlated to the manual method (r=0.95–0.99), supp…
Recurrent deficit irrigation and fruit harvest affect tree water relations and fruitlet growth in ‘valencia’ orange
2019
Background – Partial rootzone drying is an irrigation strategy known for increasing water use efficiency without significantly affecting tree water status. ‘Valencia’ oranges have a very long development period and nearly mature fruit and new fruitlets may be present at the same time on the tree, competing for water and assimilates. Objectives – The present study investigates the effect of recurrent deficit irrigation and fruit harvest on tree water status and fruitlet growth of ‘Valencia’ orange. Methods – Forty-eight adult trees were exposed to three irrigation treatments for seven years (2007–2013): irrigation with 100% of ETc (CI), continuous deficit irrigation (DI, 50% of CI) and parti…
Aldicarb residues in citrus soil, leaves and fruits
1990
Aldicarb was applied to the soil of Late Valencia orange field plots. A comparative study between four analytical methods is reported, the NPD-gas chromatography method being the best choice. The work also reports the study of accumulation and persistence of aldicarb and its biologically active metabolites in the soil, leaves and fruits (rind and pulp, separately). Residue content, at the ppb level, was found in the order leaves much greater than rind greater than pulp. Carbamate was found to accumulate mainly in its oxidized forms. Residues had reached a maximum level in about 90 days after application.
Ascorbic acid content and transcriptional profiling of genes involved in its metabolism during development of petals, leaves, and fruits of orange (C…
2021
Citrus fruit is one of the most important contributors to the ascorbic acid (AsA) intake in humans. Here, we report a comparative analysis of AsA content and transcriptional changes of genes related to its metabolism during development of petals, leaves and fruits of Valencia Late oranges (Citrus sinensis). Petals of close flowers and at anthesis contained the highest concentration of AsA. In fruits, AsA content in the flavedo reached a maximum at color break, whereas the pulp accumulated lower levels and experienced minor fluctuations during development. AsA levels in leaves were similar to those in the flavedo at breaker stage. The transcriptional profiling of AsA biosynthetic, degradatio…
A method for measuring mitochondrial mass and activity
2007
Mitochondria, responsible for the energy-generating process essential for the cell metabolism, differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs. Using fluorescent probes specific for mitochondria, Mitotracker Green (MTG) and Orange (MTO), and Confocal Laser-Scanning Microscope (CLSM), we elaborated a method to measure in vivo the mitochondrial mass and activity, in sea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variation of mitochondrial distribution and an increase of activity after fertilization.