Search results for " respiration"
showing 10 items of 208 documents
Improvement of acetaldehyde production in Zymomonas mobilis by engineering of Its aerobic metabolism
2019
Acetaldehyde is a valuable product of microbial biosynthesis, which can be used by the chemical industry as the entry point for production of various commodity chemicals. In ethanologenic microorganisms, like yeast or the bacterium Zymomonas mobilis, this compound is the immediate metabolic precursor of ethanol. In aerobic cultures of Z. mobilis, it accumulates as a volatile, inhibitory byproduct, due to the withdrawal of reducing equivalents from the alcohol dehydrogenase reaction by respiration. The active respiratory chain of Z. mobilis with its low energy-coupling efficiency is well-suited for regeneration of NAD+ under conditions when acetaldehyde, but not ethanol, is the desired catab…
Efavirenz alters mitochondrial respiratory function in cultured neuron and glial cell lines.
2015
Abstract Background The NNRTI efavirenz is among the most widely employed antiretroviral drugs. Although it is considered safe, efavirenz has been linked with several adverse effects including neurological manifestations, which appear in the majority of the patients on efavirenz-containing regimens. The molecular mechanisms responsible for these manifestations are not understood, but mounting evidence points to altered brain bioenergetics. Methods We evaluated the effect of short-term efavirenz treatment on the mitochondrial respiratory function of cultured glioblastoma and differentiated neuroblastoma cell lines using a Seahorse Extracellular Flux Analyzer. Results Incubation with efaviren…
The effectiveness of Opuntia ficus-indica mucilage edible coating on postharvest maintenance of ‘Dottato’ fig (Ficus carica L.) fruit
2017
Abstract Breba figs are highly perishable and their shelf-life is very short. In this study, breba figs (cv. ‘Dottato’) were treated with a mucilage solution of Opuntia ficus-indica cladodes, sealed in plastic bags, and stored at 4 °C for 14 days. The effect of the edible coating on the shelf-life and qualitative attributes of the fruit were evaluated by colors, content of total soluble solids, titratable acidity, total phenol, total carotenoids. Results showed that coating improves the quality of breba fig during storage. The edible coating was effective in maintaining fruit fresh weight, visual score values, fruit firmness and total carotenoid content. Coated fruit showed a significantly …
Yeast Translation Elongation Factor eIF5A Expression Is Regulated by Nutrient Availability through Different Signalling Pathways
2020
Translation elongation factor eIF5A binds to ribosomes to promote peptide bonds between problematic amino acids for the reaction like prolines. eIF5A is highly conserved and essential in eukaryotes, which usually contain two similar but differentially expressed paralogue genes. The human eIF5A-1 isoform is abundant and implicated in some cancer types
Measuring basal soil respiration across Europe: Do incubation temperature and incubation period matter?
2014
The European Commission recognises the essential role of soil biology in soil functioning and delivery of ecosystem services, but information is currently lacking evaluate of how these vary across soil and land-use types at a European scale. This study evaluated the measurement of the initial rate of soil basal respiration (BR) as a potential biological indicator of ecosystem service provision. The purpose of this study was to test ISO 16072:2002 (Soil Quality: Laboratory methods for the determining of microbial soil respiration). In the literature a range of pre-incubation temperatures (pre-inc) and experimental incubation temperatures (exp-inc) have been applied when using the ISO method …
Relationship between rhinitis duration and worsening of nasal function
2008
BackgroundWhile it is well known that asthma is characterized by airway remodeling, few studies instead have investigated this issue in patients with allergic rhinitis (AR).ObjectiveThe aim of the study was to evaluate nasal function, ie, nasal airflow, in a cohort of patients with persistent AR (PER).MethodsOne hundred patients, 50 with short-term and 50 with long-term PER, were prospectively and consecutively evaluated, clinically evaluated by visit, skin prick test, and rhinomanometry.ResultsNasal airflow values were significantly lower (median flow: 348 mL/sec) in patients with long-term rhinitis (median duration nine years) as compared to patients with short-term (median duration one y…
Neuroglobin: A Respiratory Protein of the Nervous System
2004
Nerve tissues exhibit some of the highest oxygen consumption rates found in the body. Neuroglobin, a heme protein distantly related to hemoglobin, is thought to enhance the supply of oxygen to the neurons, the eye, and some endocrine tissues. Neuroglobin may promote neuronal survival under hypoxic conditions as they occur, for example, in stroke.
Bioindicators and nutrient availability through whole soil profile under orange groves after long-term different organic fertilizations
2019
We investigated long-term (18 years) effects of three organic (cow manure (CM), poultry manure (PM), compost from agro-industry orange wastes (OW)) and one inorganic fertilization (IF) on various soil biological indicators (microbial biomass C, soil respiration, total phospholipid fatty acids (PLFAs), total bacteria, Gram-positive and Gram-negative bacteria and fungi) and nutrient contents (total and extractable organic C, total and mineral N, available P and K) along the profile of a Typic Haplustept under orange Mediterranean orchards. All fertilizers were added on the same N content basis (190 kg N ha−1 per year). Variables related to carbon cycling gradually worsened with depth, regardl…
Multirotations-CT während kontinuierlicher Beatmung: Vergleich unterschiedlicher Dichtebereiche bei gesunden Lungen und im Lavage-ARDS Modell
1999
PURPOSE In this animal study, density ranges for CT-based quantification of ventilated lung area were determined. Healthy lungs and ARDS lungs were compared during artificial respiration. MATERIAL AND METHODS CT-scans were performed in 5 anesthetized pigs using a dynamic multiscan CT option on a predefined transverse slice (slice thickness 1 mm; effective temporal resolution, 250 ms). During continuous CT acquisition, airway pressure was increased or decreased in a stepwise manner. In all images, areas of defined HU ranges were determined planimetrically. The lower threshold was set to -910 HE in all images. The upper threshold was varied from -800 HE to -200 HE in steps of 100 HE. RESULTS …
Visualization of alveolar recruitment in a porcine model of unilateral lung lavage using3He-MRI
2009
BACKGROUND: In the acute respiratory distress syndrome potentially recruitable lung volume is currently discussed. (3)He-magnetic resonance imaging ((3)He-MRI) offers the possibility to visualize alveolar recruitment directly. METHODS: With the approval of the state animal care committee, unilateral lung damage was induced in seven anesthetized pigs by saline lavage of the right lungs. The left lung served as an intraindividual control (healthy lung). Unilateral lung damage was confirmed by conventional proton MRI and spiral-CT scanning. The total aerated lung volume was determined both at a positive end-expiratory pressure (PEEP) of 0 and 10 mbar from three-dimensionally reconstructed (3)H…