Search results for " substrate"

showing 10 items of 129 documents

All-Atom simulations disclose how cytochrome reductase reshapes the substrate access/egress routes of its partner cyp450s

2020

Cytochromes P450 enzymes (CYP450s) promote the oxidative metabolism of a variety of substrates via the electrons supplied by the cytochrome P450 reductase (CPR) and upon formation of a CPR/CYP450 adduct. In spite of the pivotal regulatory importance of this process, the impact of CPR binding on the functional properties of its partner CYP450 remains elusive. By performing multiple microsecond-long all-Atom molecular dynamics simulations of a 520â »000-Atom model of a CPR/CYP450 adduct embedded in a membrane mimic, we disclose the molecular terms for their interactions, considering the aromatase (HA) enzyme as a proxy of the CYP450 family. Our study strikingly unveils that CPR binding alters…

CytochromeStereochemistryeducationPlasma protein binding-ReductaseMolecular Dynamics Simulation010402 general chemistry01 natural sciencesSubstrate SpecificityElectron Transport03 medical and health sciencesAromataseCytochrome P-450 Enzyme Systemhealth services administrationHumansddc:530General Materials Sciencecardiovascular diseasesP450 EnzymesPhysical and Theoretical Chemistryhealth care economics and organizations030304 developmental biologyNADPH-Ferrihemoprotein Reductase0303 health sciencesOxidative metabolismbiologyChemistrySubstrate (chemistry)Cytochrome P450 reductaseElectron transport chain0104 chemical sciencesAromatase; Cytochrome P-450 Enzyme System; Electron Transport; Humans; Molecular Dynamics Simulation; NADPH-Ferrihemoprotein Reductase; Protein Binding; Substrate SpecificitySettore CHIM/03 - Chimica Generale E Inorganicabiology.proteintherapeuticsProtein Binding
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Dip Pen Lithography of oligonucleotides on flexible substrates for point-of-care malaria disease testing

2015

The first step for prevention and treatment of diseases is the accurate diagnosis. However, proper diagnostic technologies are not available in developing countries due to the lack of reliable electrical power, refrigeration and trained personnel. For this reason, there is an urgent need of low cost, rapid assays not requiring any external support. By coupling such technologies to communication infrastructures, healthcare in areas without access to medical personnel would be possible. “Paper” like substrates are ideal for fabricating such devices since they are cheap, easy to degradate after use and compatible with most of existing printing technologies [1]. We had previously shown the poss…

Dip Pen Lithography oligonucleotides flexible substrates malaria
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A FAST PROCEDURE TO ESTIMATE HYDRAULIC CHARACTERISTICS OF GREENHOUSE SUBSTRATES FROM ONE-STEP OUTFLOW DATA

2011

EngineeringMeteorologyEstimation theorybusiness.industryhydraulic properties of substrates parameter estimation one-step experimentGreenhouseOutflowHorticulturebusinessActa Horticulturae
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Embedding “substrate” in environmental assessment of green roofs life cycle: evidences from an application to the whole chain in a Mediterranean site

2012

Abstract A classical LCA methodology is here applied to green roofs including in this analysis also the whole life cycle of the substrate, that represented a lack of previous LCA studies on green roofs. This inclusion will allow a complete and proper application of the LCA methodology to achieve an environmental accounting of the “green roof” performance. We have here chosen to approach the problem by analysing a specific substrate, that is used within an actual extensive green roof. Afterwards, we have applied the LCA methodology to the whole actual extensive green roof whose substrate under analysis is part of. In this manner, we achieved an environmental accounting of the “green roof” pe…

EngineeringSettore ING-IND/11 - Fisica Tecnica AmbientaleRenewable Energy Sustainability and the Environmentbusiness.industryStrategy and ManagementGreen roofEnvironmental engineeringCivil engineeringIndustrial and Manufacturing EngineeringEnvironmental accountingSubstrate (building)Work (electrical)Substrate specificityEnvironmental impact assessmentgreen roofs substrate extensive green roof life cycle assessment (LCA) life cycle approach end of life phase fertilizers MediterraneanbusinessGeneral Environmental ScienceJournal of Cleaner Production
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A low-cost compact uniplanar Quasi–Yagi printed antenna

2008

A low cost directive uniplanar broadband printed Quasi–Yagi antenna design is presented. As a particular realization, some prototypes have been designed to operate in the 2.45 GHz band. They have been then modeled, fabricated onto standard printed circuit dielectric substrate and tested successfully. For the design and the modeling processes, we have make use of FDTD based in-house developed algorithms. The obtained bandwidth is, for all the considered cases, better than 15%. The main radiation characteristics are 2–5.5 dBi gain, depending on the number of director elements, and better than 25 dB front-to-back ratio. Overall antenna size was, in any case lesser than 1 λ × 0.5 λ. © 2008 Wile…

Engineeringbusiness.industryAntenna designBandwidth (signal processing)Electrical engineeringFinite-difference time-domain methodDielectric substrateCondensed Matter PhysicsDirectivityAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsPrinted circuit boardBroadbandElectrical and Electronic EngineeringbusinessMicrowaveMicrowave and Optical Technology Letters
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Design of Yagi-like printed antennas for WLAN applications

2007

A directive broadband printed Yagi-like antenna design is presented. We have focused on the 2.45 GHz WLAN band, at which some designs have been carried out. For the design and the modelling processes, we made use of FDTD based in-house developed algorithms. Some selected prototypes have then been fabricated onto a low-cost printed circuit board dielectric substrate and tested successfully. Bandwidths higher than 10% and moderate to high (>7 dBi) gains can be obtained this way. © 2007 Wiley Periodicals, Inc. Microwave Opt Technol Lett 49: 2174–2178, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.22655

Engineeringbusiness.industryAntenna designFinite-difference time-domain methodElectrical engineeringDielectric substrateCondensed Matter PhysicsDirectivityAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsPrinted circuit boardBroadbandElectronic engineeringElectrical and Electronic EngineeringbusinessMicrowaveMicrowave and Optical Technology Letters
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Adsorption of a dye on clay and sand. Use of cyclodextrins as solubility-enhancement agents.

2007

Abstract Laboratory-scale studies were aimed at elucidating the physico-chemical aspects on the removal process of crystal violet (CV) from waters and solid substrates. The laponite clay (RD) and sand were chosen for the double aim at investigating them as CV adsorbents for water treatment and as substrates which mime the soil components. Sand is very effective in removing CV from waters. The cyclodextrins (CDs) were exploited as solubility-enhancement agents to remove CV from the solid substrates. They are powerful solvent media because they extract the CV from sand forming water-soluble CV/CD inclusion complexes and do not show affinity for sand. Optimum performance was shown by the modif…

Environmental EngineeringDyeHealth Toxicology and MutagenesisSolid substrateInclusion compoundWater Purificationchemistry.chemical_compoundAdsorptionCyclodextrinEnvironmental ChemistryCrystal violetSolubilityEquilibrium constantSettore CHIM/02 - Chimica Fisicachemistry.chemical_classificationCyclodextrinsInclusion complexChromatographyCyclodextrinChemistryPublic Health Environmental and Occupational HealthGeneral MedicineGeneral ChemistrySilicon DioxidePollutionSolventModels ChemicalSolubilityClayThermodynamicsWater treatmentAluminum SilicatesGentian VioletAdsorptionFlushing agentWater Pollutants ChemicalNuclear chemistryChemosphere
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Retarded Elimination of a High-Molecular Enzyme-Substrate-Complex after Hydroxyethyl-Starch-Infusion

1978

During a pharmacokinetic study with hydroxyethyl starch we found, that this plasma substitute induces a regular increase of serum amylase. In 54 patients after infusion of 500 ml 6% hydroxyethyl starch (HES) an increase of serum amylase was observed, which in 51 cases exceeded the upper limit of normal (190 U/l). In most cases serum amylase reached values twice as high as the basal value. Renal function influenced duration of increased serum amylase values, but did not influence maximum increases (201 ±15 U/l; mean ± SEM). In patients with advanced renal failure (GFR = 2–10 ml/min) serum amylase was still markedly elevated after 72 hours (298 ± U/l; mean ± SEM). In patients with normal rena…

Enzyme substrate complexmedicine.medical_specialtybiologyChemistryRenal functionHydroxyethyl starchExcretionBasal (phylogenetics)EndocrinologyPharmacokineticsInternal medicinemedicinebiology.proteinAmylaseLipasemedicine.drug
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Splitting of surface-related phonons in Raman spectra of self-assembled GaN nanowires

2012

cited By 2; International audience; Micro Raman spectroscopy studies have been performed on GaN nanowires grown by Plasma-Assisted Molecular Beam Epitaxy on Silicon (111) substrate. From the analysis of experimental data, the emergence of a two peaks band located near 700 cm-1 has been attributed to the Raman scattering by surface-related phonons. We have analyzed the surface character of these two modes by changing the dielectric constant of the exterior medium and some experimental parameters. Furthermore, a theoretical model describing the nanowires ensemble by means of an effective dielectric function has been used to interpret the Raman scattering results. Those numerical simulations a…

Experimental parametersRaman scatteringMaterials sciencePhononNanowireGallium nitride02 engineering and technologyDielectricDielectric functions01 natural sciencessymbols.namesakechemistry.chemical_compoundCondensed Matter::Materials ScienceExperimental observation0103 physical sciencesTheoretical models010302 applied physicsSilicon (111) substrates[PHYS]Physics [physics]Condensed matter physicsNanowiresSurface phononGallium nitride021001 nanoscience & nanotechnologyCondensed Matter PhysicschemistryDielectric propertiesRaman spectroscopysymbolsPhononsPlasma-assisted molecular beam epitaxyMicro Raman Spectroscopy0210 nano-technologyRaman spectroscopyMolecular beam epitaxyRaman scatteringSurface phononMolecular beam epitaxy
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Flamed pyrolysis titanium oxide as functional coating for Al substrate

2009

Flamed pyrolysis titanium oxide functional coating Al substrate
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