Search results for " tolerance"
showing 10 items of 760 documents
Cytotoxicity of tumor antigen specific human T cells is unimpaired by arginine depletion.
2013
Tumor-growth is often associated with the expansion of myeloid derived suppressor cells that lead to local or systemic arginine depletion via the enzyme arginase. It is generally assumed that this arginine deficiency induces a global shut-down of T cell activation with ensuing tumor immune escape. While the impact of arginine depletion on polyclonal T cell proliferation and cytokine secretion is well documented, its influence on chemotaxis, cytotoxicity and antigen specific activation of human T cells has not been demonstrated so far. We show here that chemotaxis and early calcium signaling of human T cells are unimpaired in the absence of arginine. We then analyzed CD8(+) T cell activation…
EGFP Reporters for Direct and Sensitive Detection of Mutagenic Bypass of DNA Lesions
2020
The sustainment of replication and transcription of damaged DNA is essential for cell survival under genotoxic stress
Cell proliferation and DNA breaks are involved in ultraviolet light-induced apoptosis in nucleotide excision repair-deficient Chinese hamster cells.
2002
UV light targets both membrane receptors and nuclear DNA, thus evoking signals triggering apoptosis. Although receptor-mediated apoptosis has been extensively investigated, the role of DNA damage in apoptosis is less clear. To analyze the importance of DNA damage induced by UV-C light in apoptosis, we compared nucleotide excision repair (NER)-deficient Chinese hamster ovary cells (lines 27-1 and 43-3B mutated for the repair genes ERCC3 and ERCC1, respectively) with the corresponding DNA repair-proficient fibroblasts (CHO-9 and ERCC1 complemented 43-3B cells). NER-deficient cells were hypersensitive as to the induction of apoptosis, indicating that apoptosis induced by UV-C light is due to u…
Putrescine as a signal to modulate the indispensable ABA increase under cold stress.
2009
2 páginas -- PAGS nros. 219-220
Use of nodulation pattern, stress tolerance, nodC gene amplification, RAPD-PCR and RFLP-16S rDNA analysis to discriminate genotypes of Rhizobium legu…
2005
Twenty-seven new Rhizobium isolates were obtained from root nodules of wild and crop legumes belonging to the genera Vicia, Lathyrus and Pisum from different agroecological areas in central and southern Italy. A polyphasic approach including phenotypic and genotypic techniques was used to study their diversity and their relationships with other biovars and species of rhizobia. Analysis of symbiotic properties and stress tolerance tests revealed that wild isolates, showed a wide spectrum of nodulation and a marked variation in stress tolerance compared with reference strains tested in this study. All rhizobial isolates (except for the isolate CG4 from Galega officinalis) were presumptively i…
A new fast and fault-tolerant identification algorithm for spectral databases
1995
A new method for an automatic, computer and database driven identification of UV/VIS spectra is described. It is shown that an identification algorithm must consider the spectral differences as well as their common features. The described identification method allows identifications, even if the spectra are distorted or shifted.
Suppression of humoral immune response against herpes simplex virus induced by defective strains, ts- and TK- mutants.
1988
Suppression of humoral antibody formation against HSV is not only induced by replicating Herpes simplex virus type 2 (HSV-2) but also by the defective strain ANG and the deletion mutant 1301 of Herpes simplex virus type 1 (HSV-1). Moreover, ts-mutants A, H, K, S, 1201 and 1208 of HSV-1 as well as some ts-mutants of HSV-2 and “defective-interfering” particles of HSV-1 after high multiplicity of infection-passages induced suppression. Treatment of infected mice with ACG reduced antibody-formation but did not result in suppression. UV-irradiation of the antibody producing strain Len of HSV-1 strongly reduces antibody formation and induces suppression. Experiments using a series of intertypic r…
Is pain sensitivity altered in people with Alzheimer's disease? A systematic review and meta-analysis of experimental pain research
2016
Background Clinical studies suggest people with Alzheimer's disease (AD) have altered pain sensitivity. Experimental pain research is equivocal. Objective Conduct a meta-analysis to investigate if people with AD have altered pain sensitivity compared to healthy controls (HCs). Methods Three authors searched electronic databases from inception till November 2015 for experimental pain studies in AD vs. HCs. Outcome measures were pain threshold, tolerance, pain ratings, heart rate response to noxious stimuli and the Facial Action Coding System (FACS). Random effect meta-analysis calculating Hedges' g ± 95% confidence intervals (CI) was conducted. Results Thirteen studies were identified, inclu…
Measuring Public Speaking Anxiety: Self-report, behavioral, and physiological
2021
Self-reports are typically used to assess public speaking anxiety. In this study, we examined whether self-report, observer report, and behavioral and physiological reactivity were associated with each other during a speech challenge task. A total of 95 university students completed a self-report measure of public speaking anxiety before and after the speech challenge. Speech duration (i.e., behavioral measure), physiological reactivity, as well as speech performance evaluated by the participants and observers were also recorded. The results suggest that self-reported public speaking anxiety predicts speech duration, as well as speech quality, as rated by the participants themselves and ob…
HybridS: A Scheme for Secure Distributed Data Storage in WSNs
2008
In unattended wireless sensor networks (WSNs), data is stored locally or at designated nodes upon sensing, and users can access it on demand. This paradigm can improve energy efficiency by making use of the upcoming cheap and large flash memory, as well as system robustness. Nevertheless, the security and dependability of distributed storage are critical for the applicability of such WSNs. In this paper, we propose a secure and dependable data storage scheme by taking advantages of secret sharing and Reed-Solomon code, which has computational security and yet maintains optimal data size. The extensive analysis verifies our scheme can provide secure and dependable data storage in WSNs in the…